JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB51064

重组人 Thioredoxin / TRX 蛋白 (Active) (Tag Free)

Recombinant human Thioredoxin / TRX protein (Active) (Tag Free)

Be the first to review this product! Submit a review

|

(6 Publications)

Recombinant human Thioredoxin / TRX protein (Active) (Tag Free) is a Human Full Length protein, in the 1 to 105 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS, WB.

查看别名

TRDX, TRX, TRX1, TXN, Thioredoxin, Trx, ATL-derived factor, Surface-associated sulphydryl protein, ADF, SASP

2 Images
Western blot - Recombinant human Thioredoxin / TRX protein (Active) (Tag Free) (AB51064)
  • WB

Unknown

Western blot - Recombinant human Thioredoxin / TRX protein (Active) (Tag Free) (AB51064)

All lanes:

Western blot - Anti-Thioredoxin / TRX antibody (<a href='/products/primary-antibodies/thioredoxin-trx-antibody-ab26320'>ab26320</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant human Thioredoxin / TRX protein (Active) (Tag Free) (ab51064) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 12 kDa

true

Exposure time: 30s

SDS-PAGE - Recombinant human Thioredoxin / TRX protein (Active) (Tag Free) (AB51064)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Thioredoxin / TRX protein (Active) (Tag Free) (AB51064)

3 ug of reduced ab51064, on 15% SDS-PAGE, stained with Coomassie Blue.

关键信息

纯度

>95% SDS-PAGE

Recombinant human Thioredoxin / TRX was overexpressed in E. coli and purified by using conventional chromatography techniques.

表达系统

Escherichia coli

标签

Tag free

应用

WB, SDS-PAGE, FuncS

applications

生物活性

Yes

生物学活性

Specific activity is >150 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

访问

P10599

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.4 Constituents: PBS

storage-buffer

style
left-column

搭配使用产品

Reagents, Controls & Accessories

AB234617

Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB234617)

  • 0
  • 0
Primary Antibodies

AB308567

Anti-Thioredoxin / TRX antibody [EPR22596-79] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Thioredoxin / TRX antibody [EPR22596-79] - BSA and Azide free (Detector) (AB308567)

  • 0
  • 0
Primary Antibodies

AB124954

Anti-TXNRD1 antibody [EPNCIR129]

RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-TXNRD1 antibody [EPNCIR129] (AB124954)

  • 0
  • 0
Proteins & Peptides

AB93947

Recombinant human Peroxiredoxin 4 protein (His tag N-Terminus)

SDS-PAGE - Recombinant human Peroxiredoxin 4 protein (His tag N-Terminus) (AB93947)

  • 0
  • 0
Secondary Antibodies

AB207995

Goat Anti-Rabbit IgG H&L (Biotin)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Biotin) (AB207995)

  • 0
  • 0
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab51064 can be used as a WB positive control in conjunction with <a href='/products/primary-antibodies/thioredoxin-trx-antibody-ab26320'>ab26320</a>.</p>" } } }
style
left-column

产品详情

MW confirmed by MALDI-TOF.

Concentration determined by BCA assay.

style
left-column

序列信息

[{"linker":null,"sequence":"MVKQIESKTAFQEALDAAGDKLVVVDFSATWCGPCKMIKPFFHSLSEKYSNVIFLEVDVDDCQDVASECEVKCMPTFQFFKKGQKVGEFSGANKEKLEATINELV","proteinLength":"Full Length","predictedMolecularWeight":"11.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":105,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P10599","tags":[]}]
style
left-column

性能和储存信息

形式
Liquid
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Thioredoxin (TRX) also known as TXN or Trx is a small redox protein with a molecular mass of approximately 12 kDa. It acts as an oxidoreductase enzyme facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxin is ubiquitously expressed across various tissues indicating its function in a broad range of cellular activities. The protein comprises an active site with a conserved Cys-Gly-Pro-Cys sequence critical for its reducing activity.
Biological function summary

Thioredoxin influences cellular redox homeostasis and plays direct roles in regulating cell growth and apoptosis. It participates as a major reductant in cells influencing transcription factors and enzymatic activities that depend on thiol-disulfide exchanges. Thioredoxin can interact with other components like thioredoxin reductase and NADPH to form the thioredoxin system a powerful antioxidant defense mechanism. Diseases typically arise from abnormal regulation of this system highlighting its influence on cellular survival and proliferation.

Pathways

Thioredoxin integrates into significant signaling and metabolic processes. It is an important player in the cellular response to oxidative stress and participates in signaling pathways such as the MAPK and apoptosis pathways. In these pathways thioredoxin reduces the oxidative stress transcription factor AP-1 influencing cell fate decisions. Its interaction with thioredoxin-interacting protein (TXNIP) further modulates cellular responses to oxidative environments.

Altered thioredoxin activity associates with cancer and neurodegenerative diseases. Its overexpression links to tumor progression where it promotes survival and resistance to apoptosis. Additionally in the context of neurodegenerative disorders thioredoxin’s interaction with amyloid-beta peptides implicates it in Alzheimer’s disease by mitigating oxidative stress damage. Through these conditions its role as a protector against oxidative stress remains pivotal.
style
left-column

常规信息

功能

Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions (PubMed : 17182577, PubMed : 19032234, PubMed : 2176490). Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity (PubMed : 16408020, PubMed : 17606900). Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity (PubMed : 11118054, PubMed : 9108029).. ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).

序列相似性

Belongs to the thioredoxin family.

翻译后修饰

In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.. In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.

亚细胞定位

Nucleus

style
left-column

产品实验方案

style
left-column

靶点信息

Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions (PubMed : 17182577, PubMed : 19032234, PubMed : 2176490). Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity (PubMed : 16408020, PubMed : 17606900). Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity (PubMed : 11118054, PubMed : 9108029).. ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
See full target information TXN
style
left-column

文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Clinical cancer research : an official journal of 25:7162-7174 PubMed31527169

2019

Bioactivation of Napabucasin Triggers Reactive Oxygen Species-Mediated Cancer Cell Death.

Applications

Unspecified application

Species

Unspecified reactive species

Fieke E M Froeling,Manojit Mosur Swamynathan,Astrid Deschênes,Iok In Christine Chio,Erin Brosnan,Melissa A Yao,Priya Alagesan,Matthew Lucito,Juying Li,An-Yun Chang,Lloyd C Trotman,Pascal Belleau,Youngkyu Park,Harry A Rogoff,James D Watson,David A Tuveson

Nature communications 10:4073 PubMed31501427

2019

Spatial oxidation of L-plastin downmodulates actin-based functions of tumor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Emre Balta,Robert Hardt,Jie Liang,Henning Kirchgessner,Christian Orlik,Beate Jahraus,Stefan Hillmer,Stefan Meuer,Katrin Hübner,Guido H Wabnitz,Yvonne Samstag

Journal of applied physiology (Bethesda, Md. : 198 127:858-866 PubMed31246554

2019

Characterization of extracellular redox enzyme concentrations in response to exercise in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Alex J Wadley,Gary Keane,Tom Cullen,Lynsey James,Jordan Vautrinot,Matthew Davies,Bethan Hussey,David J Hunter,Sarabjit Mastana,Adrian Holliday,Steen V Petersen,Nicolette C Bishop,Martin R Lindley,Steven J Coles

Cell chemical biology 26:449-461.e8 PubMed30713096

2019

Selective Disruption of Mitochondrial Thiol Redox State in Cells and In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Lee M Booty,Justyna M Gawel,Filip Cvetko,Stuart T Caldwell,Andrew R Hall,John F Mulvey,Andrew M James,Elizabeth C Hinchy,Tracy A Prime,Sabine Arndt,Cristiane Beninca,Thomas P Bright,Menna R Clatworthy,John R Ferdinand,Hiran A Prag,Angela Logan,Julien Prudent,Thomas Krieg,Richard C Hartley,Michael P Murphy

Chemistry Central journal 7:150 PubMed24007191

2013

Healthy ageing and depletion of intracellular glutathione influences T cell membrane thioredoxin-1 levels and cytokine secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Rita Barreto Duarte Carilho Torrao,Irundika Hk Dias,Stuart J Bennett,Christopher R Dunston,Helen R Griffiths

PloS one 6:e21926 PubMed21779355

2011

Orally active multi-functional antioxidants are neuroprotective in a rat model of light-induced retinal damage.

Applications

WB

Species

Unspecified reactive species

James Randazzo,Zifeng Zhang,Michael Hoff,Hiroyoshi Kawada,Andrew Sachs,Yang Yuan,Neena Haider,Peter Kador
View all publications
style
left-column
style
left-column
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com