重组人TGF beta 1 (mutated C33S)蛋白(Tagged) (ab271759)
Key features and details
- Expression system: Freestyle 293-F cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人TGF beta 1 (mutated C33S)蛋白(Tagged)
参阅全部 TGF beta 1 蛋白酶 -
生物活性
The ED50 measured by TGFβ1’s ability to inhibit mIL-4-dependent proliferation of HT-2 mouse helper T cells is <0.5ng/ml.
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纯度
> 90 % SDS-PAGE. -
内毒素水平
< 1.000 Eu/µg -
表达系统
Freestyle 293-F cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
LSTCKTIDME LVKRKRIEAI RGQILSKLRL ASPPSQGEVP PGPLPEAVLA LYNSTRDRVA GESAEPEPEP EADYYAKEVT RVLMVETHNE IYDKFKQSTH SIYMFFNTSE LREAVPEPVL LSRAELRLLR LKLKVEQHVE LYQKYSNNSW RYLSNRLLAP SDSPEWLSFD VTGVVRQWLS RGGEIEGFRL SAHCSCDSRD NTLQVDINGF TTGRRGDLAT IHGMNRPFLL LMATPLERAQ HLQSSRHRRA LDTNYCFSST EKNCCVRQLY IDFRKDLGWK WIHEPKGYHA NFCLGPCPYI WSLDTQYSKV LALYNQHNPG ASAAPCCVPQ ALEPLPIVYY VGRKPKVEQL SNMIVRSCKC -
预测分子量
44 kDa -
氨基酸
30 to 390 -
标签
His tag N-Terminus
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技术指标
Our Abpromise guarantee covers the use of ab271759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Lyophilized from a 0.2 µM filtered, aqueous solution.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute in sterile 0.2 M acetic acid with 0.1% BSA. Further dilutions may be made in PBS with 0.1% BSA. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
常规信息
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别名
- Cartilage-inducing factor
- CED
- Differentiation inhibiting factor
see all -
功能
Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. -
组织特异性
Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. -
疾病相关
Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision. -
序列相似性
Belongs to the TGF-beta family. -
翻译后修饰
Glycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human TGF beta 1 protein (Tagged) (ab271759)SDS-PAGE analysis of ab271759.
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Functional Studies - (ab271759)Specific activity of ab271759. The ED50 measured by TGFβ1’s ability to inhibit mIL-4-dependent proliferation of HT-2 mouse helper T cells is <0.5ng/ml.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab271759 尚未被引用在任何文献中。
