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AB271754

重组人 Tet2 蛋白 (Active) (6x His tag C-Terminus + DDDDK N-Terminus)

Recombinant human Tet2 protein (Active) (6x His tag C-Terminus + DDDDK N-Terminus)

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(1 Publication)

Recombinant human Tet2 protein (Active) (6x His tag C-Terminus + DDDDK N-Terminus) is a Human Fragment protein, in the 1129 to 2002 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

查看别名

KIAA1546, Nbla00191, TET2, Methylcytosine dioxygenase TET2

2 Images
Functional Studies - Recombinant human Tet2 protein (Active) (6x His tag C-Terminus + DDDDK N-Terminus) (AB271754)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Tet2 protein (Active) (6x His tag C-Terminus + DDDDK N-Terminus) (AB271754)

Specific activity of ab271754.

SDS-PAGE - Recombinant human Tet2 protein (Active) (6x His tag C-Terminus + DDDDK N-Terminus) (AB271754)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Tet2 protein (Active) (6x His tag C-Terminus + DDDDK N-Terminus) (AB271754)

SDS-PAGE analysis of ab271754.

关键信息

纯度

>90% SDS-PAGE

Affinity purified.

表达系统

Baculovirus infected Sf9 cells

标签

6x His tag C-Terminus DDDDK tag N-Terminus

应用

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学活性

ab271754 was tested from 0-40 ng/μl. Kit conditions were 10 ng/μl.

访问

Q6N021

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Preservative: 2.72% Imidazole Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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搭配使用产品

Reagents, Controls & Accessories

AB325994

Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color) (AB325994)

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Proteins & Peptides

AB271753

Recombinant human TET1 protein (Active) (DDDDK tag N-Terminus)

Functional Studies - Recombinant human TET1 protein (Active) (DDDDK tag N-Terminus) (AB271753)

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Proteins & Peptides

AB42619

Recombinant human JAK2 protein (His tag N-Terminus)

Functional Studies - Recombinant human JAK2 protein (His tag N-Terminus) (AB42619)

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Proteins & Peptides

AB170408

Recombinant human Dnmt3a protein (GST tag N-Terminus)

Functional Studies - Recombinant human Dnmt3a protein (AB170408)

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Proteins & Peptides

AB271756

Recombinant Human TET3 protein (DDDDK N-Terminus + His tag C-Terminus)

SDS-PAGE - Recombinant Human TET3 protein (DDDDK N-Terminus + His tag C-Terminus) (AB271756)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"100 kDa","actualMolecularWeight":null,"aminoAcidEnd":2002,"aminoAcidStart":1129,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q6N021","tags":[{"tag":"6x His","terminus":"C-Terminus"},{"tag":"DDDDK","terminus":"N-Terminus"}]}]
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Tet2 also known as ten-eleven translocation methylcytosine dioxygenase 2 is an enzyme involved in DNA demethylation. It has a molecular weight of approximately 224 kDa. This enzyme catalyzes the conversion of 5-methylcytosine to 5-hydroxymethylcytosine an important step in DNA demethylation processes. Tet2 expression occurs in various tissues with higher levels in hematopoietic cells. Its role in modification of DNA has made it a significant subject of study in epigenetic regulation.
Biological function summary

Tet2 participates in the regulation of gene expression through its impact on DNA methylation status. By promoting DNA demethylation Tet2 influences transcriptional networks that are vital for cell differentiation and development. The enzyme functions as part of a larger complex with cofactors including ascorbic acid which it requires for full enzymatic activity. Its actions affect the regulation of important processes such as self-renewal and differentiation in hematopoietic stem cells.

Pathways

The enzymatic activity of Tet2 plays a role in epigenetic pathways involved in hematopoiesis and immune function. In particular Tet2 interacts with pathways regulating chromatin structure and transcriptional control. It associates with proteins like TET1 and TET3 as they share roles in the regulation of 5-hydroxymethylcytosine levels. Through these mechanisms Tet2 contributes significantly to the maintenance of normal hematopoietic and immune cell function.

Alterations in Tet2 activity have been linked to hematological malignancies such as myelodysplastic syndromes and acute myeloid leukemia. Loss of function mutations in Tet2 lead to disruptions in hematopoietic differentiation contributing to disease pathology. Tet2 mutations can affect interactions with proteins such as DNMT3A another promoter of DNA methylation which further influence the disease course. Understanding Tet2's role in these disorders may offer potential therapeutic insights.
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常规信息

功能

Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Has a preference for 5-hydroxymethylcytosine in CpG motifs. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT.

序列相似性

Belongs to the TET family.

翻译后修饰

May be glycosylated. It is unclear whether interaction with OGT leads to GlcNAcylation. According to a report, it is not GlcNAcylated by OGT (PubMed:23353889). In contrast, another group reports GlcNAcylation by OGT in mouse ortholog.. Monoubiquitinated at Lys-1299 by the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex called CRL4(VprBP) or CUL4A-RBX1-DDB1-DCAF1/VPRBP complex; this modification promotes binding to DNA.. Acetylated (PubMed:39567688). Deacetylase HDAC6 acts as a valine sensor by binding to valine through its primate-specific SE14 repeat region and deacetylates TET2 following valine deprivation which promotes TET2-dependent DNA demethylation (PubMed:39567688).

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产品实验方案

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靶点信息

Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Has a preference for 5-hydroxymethylcytosine in CpG motifs. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT.
See full target information TET2
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

EBioMedicine 86:104333 PubMed36335665

2022

Epigenetic drug screen identified IOX1 as an inhibitor of Th17-mediated inflammation through targeting TET2.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Hu,Yanli Zou,David A Copland,Lauren P Schewitz-Bowers,Yan Li,Philippa J P Lait,Madeleine Stimpson,Zhihui Zhang,Shixin Guo,Juanran Liang,Tingting Chen,Jing Jing Li,Sujing Yuan,Shuo Li,Penghui Zhou,Yizhi Liu,Andrew D Dick,Xiaofeng Wen,Richard W J Lee,Lai Wei
View all publications
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