重组人Superoxide Dismutase 1蛋白(Active) (ab112193)

Regarding the ab112193 Recombinant Superoxide Dismutase 1 protein standard for use with ab65354 Superoxide Dismutase Activity Assay Kit:  The concentration of Cu and Zn have not been measured as they are bound to the protein and come from fermentation media while growing the cells. No exogenous metal ions have been added to the protein.

Thank you for your enquiry.

I appreciate your patience. It has taken some time to confirm details with the laboratories.

This kit is not originally designed to be run with a standard curve each time. However, if you wish to run a standard curve to calculate absolute activity (the assay is designed to calculate percent inhibition with respect to control sample), you can try using the human recombinant active SOD enzyme

ab112193
https://www.abcam.com/Recombinant-human-Superoxide-Dismutase-1-protein-Active-ab112193.html (or use the following: https://www.abcam.com/Recombinant-human-Superoxide-Dismutase-1-protein-Active-ab112193.html).

I hope this will be helpful. If you have any further questions, please do not hesitate to contact me.

The ab65354 Superoxide Dismutase Activity Colorimetric Assay Kit can be used without a standard since it is reporting as % inhibition in your sample. Blank 1 does not contain any inhibition, and your sample will have some percentage of inhibition. Blank 2 and Blank 3 are background values so remember to minus these values when doing the calculation. Thus, the more SOD in a sample the less WST-1 formazan produced (which was dependent on Xanthine Oxidase (XO) activity).


If you want to measure the amount of SOD in your sample, you can use a purified protein as a standard (ab112193 SOD full length protein) and then treat the standard just as you would treat a sample and calculate the amount using a standard curve. Here are the details of the standard.


For your information, a 96-well plate is also not included in the kit but you can use a standard flat bottom plate such as the one sold by Greiner Bio one (#655-101) or any other standard polystyrene plate.

We recommend use of ab112193 SOD protein as standard for this kit.

Thank you for your enquiry.

I have investigated this and can confirm that we would recommend to use ab112193 SOD protein if you need to use a standard with the ab65354 kit. I can recommend to review the online datasheet for further information:

https://www.abcam.com/Recombinant-human-Superoxide-Dismutase-1-protein-Active-ab112193.html (or use the following: https://www.abcam.com/Recombinant-human-Superoxide-Dismutase-1-protein-Active-ab112193.html).

I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

Thank you for contacting Abcam.

Recombinant Human Cu/Zn Superoxide Dismutase produced in E.Coli is a homodimer, non-glycosylated polypeptide chain containing 2 x 154 amino acids and having a total molecular mass of 31,608 Dalton.

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Lys-Ala-Val.

I hope this information has been helpful for you.

Please let me know if you have any other questions.