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AB140417

Recombinant Human Sumo 1 protein

Recombinant Human Sumo 1 protein

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Recombinant Human Sumo 1 protein is a Human Full Length protein, in the 2 to 97 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, WB.

查看别名

SMT3C, SMT3H3, UBL1, OK/SW-cl.43, SUMO1, Small ubiquitin-related modifier 1, SUMO-1, GAP-modifying protein 1, SMT3 homolog 3, Sentrin, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein SMT3C, Ubiquitin-like protein UBL1, GMP1, Smt3C

5 Images
Western blot - Recombinant Human Sumo 1 protein (AB140417)
  • WB

Lab

Western blot - Recombinant Human Sumo 1 protein (AB140417)

**Blocking buffer : ** 5% NFDM/TBST This data was developed using ab109005, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (<a href='/products/primary-antibodies/sumo-2-sumo-3-antibody-epr4602-ab109005'>ab109005</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human Sumo 1 protein (ab140417) at 0.01 µg

Lane 2:

Western blot - Recombinant Human Sumo 2 protein (<a href='/products/proteins-peptides/recombinant-human-sumo-2-protein-ab140420'>ab140420</a>) at 0.01 µg

Lane 3:

Recombinant Human Sumo 3 protein (ab140414) at 0.01 µg

Lane 4:

Western blot - Recombinant Human Sumo 4 protein (<a href='/products/proteins-peptides/recombinant-human-sumo-4-protein-ab157025'>ab157025</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 12 kDa

Observed band size: 16 kDa

true

Exposure time: 20s

Western blot - Recombinant Human Sumo 1 protein (AB140417)
  • WB

Lab

Western blot - Recombinant Human Sumo 1 protein (AB140417)

**Blocking buffer : ** 5% NFDM/TBST This data was developed using ab109196, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (<a href='/products/primary-antibodies/sumo-2-sumo-3-sumo-4-antibody-epr3002-ab109196'>ab109196</a>) at 1/200 dilution

Lane 1:

Western blot - Recombinant Human Sumo 1 protein (ab140417) at 0.01 µg

Lane 2:

Western blot - Recombinant Human Sumo 2 protein (<a href='/products/proteins-peptides/recombinant-human-sumo-2-protein-ab140420'>ab140420</a>) at 0.01 µg

Lane 3:

Recombinant Human Sumo 3 protein (ab140414) at 0.01 µg

Lane 4:

Western blot - Recombinant Human Sumo 4 protein (<a href='/products/proteins-peptides/recombinant-human-sumo-4-protein-ab157025'>ab157025</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 12 kDa

Observed band size: 16 kDa

true

Exposure time: 20s

Western blot - Recombinant Human Sumo 1 protein (AB140417)
  • WB

Lab

Western blot - Recombinant Human Sumo 1 protein (AB140417)

**Blocking buffer : ** 5% NFDM/TBST This data was developed using ab133352, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Sumo 1 antibody [EP298] (<a href='/products/primary-antibodies/sumo-1-antibody-ep298-ab133352'>ab133352</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human Sumo 1 protein (ab140417) at 0.01 µg

Lane 2:

Western blot - Recombinant Human Sumo 2 protein (<a href='/products/proteins-peptides/recombinant-human-sumo-2-protein-ab140420'>ab140420</a>) at 0.01 µg

Lane 3:

Recombinant Human Sumo 3 protein (ab140414) at 0.01 µg

Lane 4:

Western blot - Recombinant Human Sumo 4 protein (<a href='/products/proteins-peptides/recombinant-human-sumo-4-protein-ab157025'>ab157025</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 11 kDa

Observed band size: 16 kDa

true

Exposure time: 10s

Western blot - Recombinant Human Sumo 1 protein (AB140417)
  • WB

Lab

Western blot - Recombinant Human Sumo 1 protein (AB140417)

**Blocking buffer : ** 5% NFDM/TBST This data was developed using ab126606, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (<a href='/products/primary-antibodies/sumo-2-sumo-3-sumo-4-antibody-epr7163-ab126606'>ab126606</a>) at 1/5000 dilution

Lane 1:

Western blot - Recombinant Human Sumo 1 protein (ab140417) at 0.01 µg

Lane 2:

Western blot - Recombinant Human Sumo 2 protein (<a href='/products/proteins-peptides/recombinant-human-sumo-2-protein-ab140420'>ab140420</a>) at 0.01 µg

Lane 3:

Recombinant Human Sumo 3 protein (ab140414) at 0.01 µg

Lane 4:

Western blot - Recombinant Human Sumo 4 protein (<a href='/products/proteins-peptides/recombinant-human-sumo-4-protein-ab157025'>ab157025</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 11 kDa

Observed band size: 16 kDa

true

Exposure time: 3s

SDS-PAGE - Recombinant Human Sumo 1 protein (AB140417)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Sumo 1 protein (AB140417)

SDS-PAGE analysis of ab140417.

关键信息

纯度

>90% Densitometry

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

SDS-PAGE, WB

applications

生物活性

No

访问

P63165

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"SDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGG","proteinLength":"Full Length","predictedMolecularWeight":"19 kDa","actualMolecularWeight":null,"aminoAcidEnd":97,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P63165","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SUMO 1 also known as small ubiquitin-like modifier 1 is part of the protein family involved in post-translational modification. The protein mechanically adds a SUMO group to target proteins through sumoylation a process similar to ubiquitination. SUMO 1 typically has a molecular mass of around 11 kDa. It expresses in various tissues and cells including the nucleus and cytoplasm of eukaryotic cells. In addition it is important in processes like nuclear transport transcriptional regulation and protein stabilization.
Biological function summary

SUMO 1 contributes significantly to maintaining cellular homeostasis. It is an integral part of a SUMOylation complex that modifies other proteins to alter their function localization or interactions. Through its modification actions SUMO 1 affects processes such as DNA repair and the cell cycle. By interacting with components of the nuclear pore complex and transcription factors SUMO 1 modulates essential biological activities at multiple levels within the cell.

Pathways

This protein plays a significant role in the PI3K/Akt pathway and Wnt signaling. SUMO 1 associates with proteins like RanGAP1 and various transcription factors highlighting the complexity of its regulatory actions. These associations are important for the regulation of cell survival proliferation and differentiation. SUMOylation by SUMO 1 has been linked to an interference with phosphorylation showing its potential influence on cell signaling pathways.

The dysregulation of SUMO 1 is seen in cancer and neurodegenerative diseases. For instance altered SUMOylation patterns contribute to the progression of certain cancers. Furthermore SUMO 1 interacts with proteins like p53 and these interactions can affect tumor suppression and cell cycle regulation. In neurodegenerative disorders disturbed SUMOylation is associated with protein aggregation and neuronal damage implicating SUMO 1 in diseases like Alzheimer's.
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特殊说明

形式

Liquid

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常规信息

功能

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalently attached to the voltage-gated potassium channel KCNB1; this modulates the gating characteristics of KCNB1 (PubMed : 19223394). Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. Covalently attached to ZFHX3 (PubMed : 24651376).

序列相似性

Belongs to the ubiquitin family. SUMO subfamily.

翻译后修饰

Cleavage of precursor form by SENP1 or SENP2 is necessary for function.. Polymeric SUMO1 chains undergo polyubiquitination by RNF4.

亚细胞定位

Nucleus membrane

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产品实验方案

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靶点信息

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalently attached to the voltage-gated potassium channel KCNB1; this modulates the gating characteristics of KCNB1 (PubMed : 19223394). Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. Covalently attached to ZFHX3 (PubMed : 24651376).
See full target information SUMO1
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