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AB78681

重组人 Stromal interaction molecule 1 蛋白 (Calmodulin tag N-Terminus)

Recombinant Human Stromal interaction molecule 1 protein (Calmodulin tag N-Terminus)

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Recombinant Human Stromal interaction molecule 1 protein (Calmodulin tag N-Terminus) is a Human Fragment protein, in the 23 to 213 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

查看别名

GOK, STIM1, Stromal interaction molecule 1

1 Images
SDS-PAGE - Recombinant Human Stromal interaction molecule 1 protein (Calmodulin tag N-Terminus) (AB78681)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Stromal interaction molecule 1 protein (Calmodulin tag N-Terminus) (AB78681)

15% SDS-PAGE showing ab68681 at approximately 38kDa (3μg).

关键信息

纯度

>90% SDS-PAGE

表达系统

Escherichia coli

标签

Calmodulin tag N-Terminus

应用

SDS-PAGE

applications

生物活性

No

访问

Q13586

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.5 Constituents: 0.242% Tris

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAKGSMLSHSHSEKATGTSSGANSEESTAAEFCRIDKPLCHSEDEKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVVQWLITYVELPQYEETFRKLQLSGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKD","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":213,"aminoAcidStart":23,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q13586","tags":[{"tag":"Calmodulin","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Stromal interaction molecule 1 also known as STIM1 is a single-pass transmembrane protein primarily involved in the regulation of calcium ion entry into cells. It acts as a sensor of calcium levels inside the endoplasmic reticulum. STIM1 is about 90 kDa in mass and expresses in various tissues including the immune system skeletal muscle and vascular smooth muscle. The protein anchors in the membrane of the endoplasmic reticulum where it responds to the depletion of calcium by communicating with plasma membrane channels to activate calcium influx.
Biological function summary

STIM1 plays a critical role in the store-operated calcium entry (SOCE) mechanism a significant process in cellular calcium signaling. STIM1 senses calcium concentration within the endoplasmic reticulum and upon depletion aggregates and migrates to the plasma membrane to interact with Orai1 part of the calcium release-activated calcium (CRAC) channel complex. This interaction opens the channel allowing calcium ions to flow into the cytosol which is necessary for various cellular functions such as gene expression and cell proliferation.

Pathways

STIM1 functions integrally in the calcium signaling pathway a system central to numerous physiological processes. Here STIM1 ensures appropriate calcium ion concentrations facilitate activation of various transcription factors and enzymes. It closely associates with Orai1 triggering the CRAC channel activation in response to the endoplasmic reticulum calcium levels. Additionally STIM1 interacts with other signaling molecules like protein kinase C coordinating between different pathways to maintain cellular homeostasis.

STIM1 dysfunction links to conditions such as immunodeficiency and muscle disorders. Mutations in STIM1 can lead to combined immunodeficiency characterized by impaired immune responses due to faulty calcium influx in lymphocytes. Additionally aberrations in STIM1 activity associate with tubular aggregate myopathy a muscle disorder where abnormal calcium handling in muscle cells leads to muscle weakness and pain. In these diseases the interaction between STIM1 and Orai1 is often disrupted highlighting their roles in maintaining physiological processes.
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特殊说明

形式

Liquid

附加说明

ab78681 is purified using conventional chromatography techniques.

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常规信息

功能

Acts as a Ca(2+) sensor that gates two major inward rectifying Ca(2+) channels at the plasma membrane : Ca(2+) release-activated Ca(2+) (CRAC) channels and arachidonate-regulated Ca(2+)-selective (ARC) channels (PubMed : 15866891, PubMed : 16005298, PubMed : 16208375, PubMed : 16537481, PubMed : 16733527, PubMed : 16766533, PubMed : 16807233, PubMed : 18854159, PubMed : 19182790, PubMed : 19249086, PubMed : 19622606, PubMed : 19706554, PubMed : 22464749, PubMed : 24069340, PubMed : 24351972, PubMed : 24591628, PubMed : 25326555, PubMed : 26322679, PubMed : 28219928, PubMed : 32415068). Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates CRAC channel pore-forming subunits ORA1, ORA2 and ORAI3 to generate sustained and oscillatory Ca(2+) entry (PubMed : 16208375, PubMed : 16537481, PubMed : 32415068). Involved in enamel formation (PubMed : 24621671).

翻译后修饰

Glycosylation is required for cell surface expression.. Phosphorylated predominantly on Ser residues.

亚细胞定位

Cytoskeleton

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产品实验方案

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靶点信息

Acts as a Ca(2+) sensor that gates two major inward rectifying Ca(2+) channels at the plasma membrane : Ca(2+) release-activated Ca(2+) (CRAC) channels and arachidonate-regulated Ca(2+)-selective (ARC) channels (PubMed : 15866891, PubMed : 16005298, PubMed : 16208375, PubMed : 16537481, PubMed : 16733527, PubMed : 16766533, PubMed : 16807233, PubMed : 18854159, PubMed : 19182790, PubMed : 19249086, PubMed : 19622606, PubMed : 19706554, PubMed : 22464749, PubMed : 24069340, PubMed : 24351972, PubMed : 24591628, PubMed : 25326555, PubMed : 26322679, PubMed : 28219928, PubMed : 32415068). Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates CRAC channel pore-forming subunits ORA1, ORA2 and ORAI3 to generate sustained and oscillatory Ca(2+) entry (PubMed : 16208375, PubMed : 16537481, PubMed : 32415068). Involved in enamel formation (PubMed : 24621671).
See full target information STIM1
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搭配使用产品

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