JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB84627

重组 Human STAT5a protein (His tag N-Terminus)

Recombinant Human STAT5a protein (His tag N-Terminus)

Be the first to review this product! Submit a review

|

(1 Publication)

Recombinant Human STAT5a protein (His tag N-Terminus) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB.

查看别名

STAT5, STAT5A, Signal transducer and activator of transcription 5A

2 Images
Western blot - Recombinant Human STAT5a protein (His tag N-Terminus) (AB84627)
  • WB

Unknown

Western blot - Recombinant Human STAT5a protein (His tag N-Terminus) (AB84627)

All lanes:

Western blot - Anti-STAT5a antibody [E289] (<a href='/products/primary-antibodies/stat5a-antibody-e289-ab32043'>ab32043</a>) at 1/1000 dilution

All lanes:

Western blot - Recombinant Human STAT5a protein (His tag N-Terminus) (ab84627) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 90 kDa

true

Exposure time: 20s

SDS-PAGE - Recombinant Human STAT5a protein (His tag N-Terminus) (AB84627)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human STAT5a protein (His tag N-Terminus) (AB84627)

SDS Page analysis of ab84627

关键信息

纯度

>90% SDS-PAGE

Purity >90% as determined by densitometry. Affinity purified.

表达系统

Baculovirus infected Sf9 cells

标签

His tag N-Terminus

应用

SDS-PAGE, WB

applications

生物活性

No

访问

P42229

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.00308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF

storage-buffer

style
left-column

搭配使用产品

Reagents, Controls & Accessories

AB234617

Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB234617)

  • 0
  • 0
Proteins & Peptides

AB182829

Recombinant Human STAT5b protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human STAT5b protein (GST tag N-Terminus) (AB182829)

  • 0
  • 0
Proteins & Peptides

AB43616

Recombinant Human STAT1 protein (GST tag N-Terminus)

  • 0
  • 0
Proteins & Peptides

AB43617

Recombinant Human STAT2 protein (GST tag N-Terminus)

Western blot - Recombinant Human STAT2 protein (GST tag N-Terminus) (AB43617)

  • 0
  • 0
Proteins & Peptides

AB84634

Recombinant Human STAT4 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human STAT4 protein (His tag N-Terminus) (AB84634)

  • 0
  • 0
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p><a href='/products/primary-antibodies/stat5a-antibody-e289-ab32043'>ab32043</a> can be used as a WB positive control in conjunction with ab84627.</p>" } } }
style
left-column
style
left-column

序列信息

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P42229","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

STAT5a also known as Signal Transducer and Activator of Transcription 5A acts as a transcription factor in the cell. STAT5a weighs approximately 90 kDa and expresses in various tissues including the liver muscle and hematopoietic cells. It plays a central role in mediating cellular responses to cytokines and growth factors particularly those in the interleukin-2 (IL-2) family and growth hormone (GH).
Biological function summary

Many systems use STAT5a to regulate cell proliferation differentiation and survival. It often forms a complex with other proteins like STAT5b which is a closely related counterpart and both contribute to similar cellular processes. STAT5a can dimerize upon activation allowing it to move to the cell nucleus to influence gene expression directly.

Pathways

Researchers place STAT5a prominently in the JAK-STAT signaling pathway. This pathway is essential for transmitting information received from extracellular chemical signals to the cell nucleus resulting in the activation of genes controlling cellular functions. STAT5a works together with Janus kinases (JAKs) to mediate various biological responses including hormone signaling and immune function. It also interacts with proteins like IL-2 receptor subunits which are involved in many cytokine-driven processes.

Disturbances in STAT5a activity link specifically with conditions like leukemia and breast cancer. Overactivation or mutation can lead to uncontrolled cell growth and survival contributing to these diseases. In leukemia STAT5a frequently partners with other proteins like BCR-ABL which is known for its role in chronic myeloid leukemia influencing oncogenic pathways. In breast cancer alterations in STAT5a signaling may interact with estrogen and progesterone receptors affecting tumor progression and response to therapies.
style
left-column

常规信息

功能

Carries out a dual function : signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.

序列相似性

Belongs to the transcription factor STAT family.

翻译后修饰

Tyrosine phosphorylated in response to KITLG/SCF, IL2, IL3, IL7, IL15, CSF2/GMCSF, GH1, PRL, EPO and THPO (By similarity). Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus (PubMed:21135090). Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity (By similarity). Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3 (PubMed:14504097). Alternatively, can be phosphorylated by JAK2 at Tyr-694 (PubMed:12529425).. ISGylated.

style
left-column

产品实验方案

style
left-column

靶点信息

Carries out a dual function : signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
See full target information STAT5A
style
left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Carcinogenesis 35:2244-53 PubMed24925029

2014

Inhibition of STAT5a by Naa10p contributes to decreased breast cancer metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zeng,Li Min,Yong Han,Lin Meng,Caiyun Liu,Yuntao Xie,Bin Dong,Lixin Wang,Beihai Jiang,Huiyu Xu,Qing Zhuang,Chuanke Zhao,Like Qu,Chengchao Shou
View all publications
style
left-column
style
left-column
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com