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AB108129

重组人sRANKL蛋白

Recombinant Human sRANKL protein

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Recombinant Human sRANKL protein is a Human Full Length protein, in the 140 to 317 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, Mass Spec.

查看别名

CD254, OPGL, RANKL, TRANCE, TNFSF11, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, ODF

1 Images
SDS-PAGE - Recombinant Human sRANKL protein (AB108129)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human sRANKL protein (AB108129)

15% SDS-PAGE analysis of 3µg ab108129.

关键信息

纯度

>80% SDS-PAGE

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

SDS-PAGE, Mass Spec

applications

生物活性

No

访问

O14788

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMIRAEKAMVDGSWLDLAKRSKLEAQPFAHLTINATDIPSGSHKVSLSSWYHDRGWAKISNMTFSNGKLIVNQDGFYYLYANICFRHHETSGDLATEYLQLMVYVTKTSIKIPSSHTLMKGGSTKYWSGNSEFHFYSINVGGFFKLRSGEEISIEVSNPSLLDPDQDATYFGAFKVRDID","proteinLength":"Full Length","predictedMolecularWeight":"22.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":317,"aminoAcidStart":140,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O14788","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SRANKL also known as soluble receptor activator of nuclear factor kappa-B ligand is an essential protein involved in bone metabolism. It acts as a cytokine and is a member of the tumor necrosis factor (TNF) superfamily. sRANKL is derived from the membrane-bound form of RANKL by proteolytic cleavage and has a molecular weight of approximately 18 kDa. This protein is predominantly found in osteoblasts osteocytes and T-cells where it plays a critical role in physiological processes.
Biological function summary

SRANKL facilitates the differentiation and activation of osteoclasts which are cells responsible for bone resorption. It binds to its receptor RANK on osteoclast precursors promoting their maturation into fully functional osteoclasts. This binding process involves complex formation and interaction with another molecule called osteoprotegerin (OPG) which acts as a decoy receptor regulating sRANKL activity and therefore controlling bone resorption.

Pathways

SRANKL plays a fundamental role in the RANK/RANKL/OPG signaling pathway which is important for maintaining bone homeostasis. This pathway regulates the balance between bone formation and resorption. It also interacts with NF-kappaB signaling a pathway that influences inflammation and immune response. sRANKL through these pathways closely connects with proteins such as TRAF6 which mediates downstream signaling leading to osteoclastogenesis.

Alterations in the sRANKL pathway impact bone-related diseases such as osteoporosis and rheumatoid arthritis. In osteoporosis increased levels of sRANKL enhance osteoclast activity leading to excessive bone resorption and thereby weakening bones. In rheumatoid arthritis the interaction between sRANKL and RANK promotes inflammation and joint destruction. This protein is linked to OPG as variations in their ratio influence disease development and progression.
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特殊说明

形式

Liquid

附加说明

ab108129 was purified using conventional chromatography.

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常规信息

功能

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (PubMed : 22664871). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (By similarity).

序列相似性

Belongs to the tumor necrosis factor family.

翻译后修饰

The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17.

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产品实验方案

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靶点信息

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (PubMed : 22664871). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (By similarity).
See full target information TNFSF11
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搭配使用产品

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Primary Antibodies

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Secondary Antibodies

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Assay Kits

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