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AB152700

重组人 Spastin 蛋白 (GST tag N-Terminus)

Recombinant Human Spastin protein (GST tag N-Terminus)

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(1 Publication)

Recombinant Human Spastin protein (GST tag N-Terminus) is a Human Fragment protein, in the 200 to 304 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

查看别名

ADPSP, FSP2, KIAA1083, SPG4, SPAST, Spastin, Spastic paraplegia 4 protein

1 Images
SDS-PAGE - Recombinant Human Spastin protein (GST tag N-Terminus) (AB152700)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Spastin protein (GST tag N-Terminus) (AB152700)

12.5% SDS-PAGE analysis of ab152700 stained with Coomassie Blue.

关键信息

表达系统

Wheat germ

标签

GST tag N-Terminus

应用

SDS-PAGE, WB, ELISA

applications

生物活性

No

访问

Q9UBP0

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"PVLPFSKSQTDVYNDSTNLACRNGHLQSESGAVPKRKDPLTHTSNSLPRSKTVMKTGSAGLSGHHRAPSYSGLSMVSGVKQGSGPAPTTHKGTPKTNRTNKPSTP","proteinLength":"Fragment","predictedMolecularWeight":"37.29 kDa","actualMolecularWeight":null,"aminoAcidEnd":304,"aminoAcidStart":200,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q9UBP0","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Spastin also known as SPG4 is a microtubule-severing ATPase that plays an important role in cellular mechanics by cutting microtubules in cells. It weighs approximately 68 kDa and is present mainly in neural tissue but can also be found in other tissues. The protein's localization includes the cytoplasm and is associated with centrosomes and subcellular regions with dense microtubule networks.
Biological function summary

Spastin participates in regulating microtubule dynamics. It plays a critical role in axonal growth and maintenance by modulating microtubule lengths and branching. Spastin does not function alone; it forms part of the AAA protein family functioning in significant processes like neurogenesis and neural maintenance. Its activity affects neural cell structure and transport by influencing cytoskeletal rearrangement.

Pathways

Any disturbances in Spastin function can impact the microtubule dynamics pathway and the axonal transport pathway. Spastin interacts closely with proteins such as Tubulin and Katanin which are also involved in microtubule severing and organization. Proper Spastin function is necessary to maintain intracellular transport and signal transduction critical for neural cell health and function.

Alterations in Spastin activity lead to neurodegenerative conditions like Hereditary Spastic Paraplegia (HSP). This disorder often relates to mutations in the SPG4 gene which encodes Spastin affecting motor neuron functions. In HSP disrupted Spastin function correlates with deficiencies in proteins such as Tubulin exacerbating neuroaxonal deficits and influencing disease progression.
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特殊说明

形式

Liquid

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常规信息

功能

ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated (PubMed : 11809724, PubMed : 15716377, PubMed : 16219033, PubMed : 17389232, PubMed : 20530212, PubMed : 22637577, PubMed : 26875866). Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails : severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed : 26875866). Severing activity is not dependent on tubulin acetylation or detyrosination (PubMed : 26875866). Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. It is critical for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia. SPAST is involved in abscission step of cytokinesis and nuclear envelope reassembly during anaphase in cooperation with the ESCRT-III complex (PubMed : 19000169, PubMed : 21310966, PubMed : 26040712). Recruited at the midbody, probably by IST1, and participates in membrane fission during abscission together with the ESCRT-III complex (PubMed : 21310966). Recruited to the nuclear membrane by IST1 and mediates microtubule severing, promoting nuclear envelope sealing and mitotic spindle disassembly during late anaphase (PubMed : 26040712). Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and endosome recycling (PubMed : 23897888). Recruited by IST1 to endosomes and regulates early endosomal tubulation and recycling by mediating microtubule severing (PubMed : 23897888). Probably plays a role in axon growth and the formation of axonal branches (PubMed : 15716377).. Isoform 1. Involved in lipid metabolism by regulating the size and distribution of lipid droplets.

序列相似性

Belongs to the AAA ATPase family. Spastin subfamily.

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产品实验方案

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靶点信息

ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated (PubMed : 11809724, PubMed : 15716377, PubMed : 16219033, PubMed : 17389232, PubMed : 20530212, PubMed : 22637577, PubMed : 26875866). Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails : severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed : 26875866). Severing activity is not dependent on tubulin acetylation or detyrosination (PubMed : 26875866). Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. It is critical for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia. SPAST is involved in abscission step of cytokinesis and nuclear envelope reassembly during anaphase in cooperation with the ESCRT-III complex (PubMed : 19000169, PubMed : 21310966, PubMed : 26040712). Recruited at the midbody, probably by IST1, and participates in membrane fission during abscission together with the ESCRT-III complex (PubMed : 21310966). Recruited to the nuclear membrane by IST1 and mediates microtubule severing, promoting nuclear envelope sealing and mitotic spindle disassembly during late anaphase (PubMed : 26040712). Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and endosome recycling (PubMed : 23897888). Recruited by IST1 to endosomes and regulates early endosomal tubulation and recycling by mediating microtubule severing (PubMed : 23897888). Probably plays a role in axon growth and the formation of axonal branches (PubMed : 15716377).. Isoform 1. Involved in lipid metabolism by regulating the size and distribution of lipid droplets.
See full target information SPAST
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Neural regeneration research 14:1271-1279 PubMed30804259

2019

Effect of exogenous spastin combined with polyethylene glycol on sciatic nerve injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yao-Fa Lin,Zheng Xie,Jun Zhou,Hui-Hao Chen,Wan-Wan Shao,Hao-Dong Lin
View all publications
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搭配使用产品

Primary Antibodies

AB77144

Anti-Spastin antibody [Sp 6C6]

KO Validated

Immunoprecipitation - Anti-Spastin antibody [Sp 6C6] (AB77144)

  • 4
  • 1
Cell Lines & Lysates

AB267238

Human SPAST (Spastin) knockout HEK-293T cell line

Advanced Validation

Western blot - Human SPAST (Spastin) knockout HEK-293T cell line (AB267238)

  • 0
  • 0
Primary Antibodies

AB228006

Anti-XAB2 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XAB2 antibody - N-terminal (AB228006)

  • 0
  • 0
Primary Antibodies

AB229338

Anti-Lyl1 antibody - C-terminal

Western blot - Anti-Lyl1 antibody - C-terminal (AB229338)

  • 0
  • 0

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