重组人SPARC蛋白(ab107952)
Key features and details
- Expression system: CHO cells
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人SPARC蛋白
参阅全部 SPARC 蛋白酶 -
生物活性
Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3 cells, with an IC50 of 0.5-0.7 μg/mL.
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纯度
> 98 % SDS-PAGE.
Purity is greater than 98% by SDS-PAGE gel and HPLC analyses. -
内毒素水平
< 1.000 Eu/µg -
表达系统
CHO cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
APQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFDDGAEETEEEVV AENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNK TFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIPPCLDSELTEFPLRM RDWLKNVLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLA RDFEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTT RFFETCDLDNDKYIALDEWAGCFGIKQKDIDKDLVI -
预测分子量
44 kDa -
氨基酸
18 to 303
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab107952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituent: 0.164% Sodium phosphate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute in water to a concentration of 0.1-1.0 mg/ml
常规信息
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别名
- AA517111
- Basement membrane protein 40
- Basement-membrane protein 40
see all -
功能
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. -
序列相似性
Belongs to the SPARC family.
Contains 1 EF-hand domain.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain. -
发展阶段
Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair. -
细胞定位
Secreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab107952 尚未被引用在任何文献中。
