重组人SPAM1蛋白(ab174000)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 92% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组人SPAM1蛋白
参阅全部 SPAM1 蛋白酶 -
生物活性
The activity of ab174000 is measured by its ability to hydrolyze HA in turbidimetric assay (45 minute assay). The specific activity is >40,000 U/mg. Unit Definition: One unit of Hyaluronidase activity will cause a change in A600 of 0.330 per minute at pH 5.35 at 37 °C in a 2.0 ml reaction mixture.
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纯度
> 92 % SDS-PAGE. -
内毒素水平
< 1.000 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
LNFRAPPVIPNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATG QGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYM PVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEAT EKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYN GSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREA IRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASG IVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQE QGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKF YCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQIFY -
预测分子量
52 kDa including tags -
氨基酸
36 to 482 -
标签
His tag C-Terminus -
额外的序列信息
GenBank: AAH26163.1
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab174000 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Lyophilized -
补充说明
This product was previously labelled as Hyaluronidase PH20. The protein migrates as 64-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C. Reconstitute for long term storage.
pH: 7.4
Constituents: 5% Trehalose, 0.61% Tris, 0.58% Sodium chloride
pH 7.0 - 7.4This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute with 20% glycerol to a concentration of 100 µg/ml.
常规信息
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别名
- epididymis secretory sperm binding protein Li 96n
- HEL-S-96n
- HYA1
see all -
功能
Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. -
组织特异性
Testis. -
序列相似性
Belongs to the glycosyl hydrolase 56 family. -
翻译后修饰
N-glycosylated. -
细胞定位
Cell membrane. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human SPAM1 protein (ab174000)
SDS-PAGE analysis of reduced ab174000 stained overnight with Coomassie Blue.
DTT-reduced Protein migrates as 64-66 kDa due to glycosylation.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab174000 被引用在 1 文献中.
- Sullivan WJ et al. Extracellular Matrix Remodeling Regulates Glucose Metabolism through TXNIP Destabilization. Cell 175:117-132.e21 (2018). PubMed: 30197082
