JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB79950

重组人SOX2蛋白

Recombinant Human SOX2 protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human SOX2 protein is a Human Full Length protein, in the 1 to 317 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, HPLC, sELISA, WB.

查看别名

Transcription factor SOX-2, SOX2

2 Images
Sandwich ELISA - Recombinant Human SOX2 protein (AB79950)
  • sELISA

Unknown

Sandwich ELISA - Recombinant Human SOX2 protein (AB79950)

Standard Curve for SOX2 (Analyte : SOX2 protein (Human) (ab79950)); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [57CT23.3.4] to SOX2 (ab75485) at 0.2μg/ml and Detector Antibody Rabbit monoclonal [EPR3131] to SOX2 (ab92494) at 0.5μg/ml.

Western blot - Recombinant Human SOX2 protein (AB79950)
  • WB

Unknown

Western blot - Recombinant Human SOX2 protein (AB79950)

All lanes:

Western blot - Anti-SOX2 antibody (<a href='/products/primary-antibodies/sox2-antibody-ab97959'>ab97959</a>) at 1/1000 dilution

All lanes:

Western blot - Recombinant Human SOX2 protein (ab79950) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 34 kDa

true

Exposure time: 30s

关键信息

纯度

>95% SDS-PAGE

表达系统

Escherichia coli

标签

Tag free

应用

SDS-PAGE, sELISA, WB, HPLC

applications

生物活性

No

访问

P48431

不含动物源

No

不含载体蛋白

No

种属

Human

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab79950 can be used as a WB positive control in conjunction with <a href='/products/primary-antibodies/sox2-antibody-ab97959'>ab97959</a>.</p>" } } }
style
left-column
style
left-column

序列信息

[{"sequence":"MYNMMETELKPPGPQQTSGGGGGNSTAAAAGGNQKNSPDRVKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHMKEHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDSYAHMNGWSNGSYSMMQDQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYMNGSPTYSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMISMYLPGAEVPEPAAPSRLHMSQHYQSGPVPGTAINGTLPLSHM","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":317,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P48431","tags":[]}]
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的长期储存条件
-20°C
储存信息
Stable for 12 months at -20°C
False
style
left-column
style
left-column

特殊说明

形式

Lyophilized

附加说明

ab79950 is greater than 95% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per µg (1EU/µg).

style
left-column

常规信息

功能

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Binds to the proximal enhancer region of NANOG (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed : 18035408). Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). May function as a switch in neuronal development (By similarity).

翻译后修饰

Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.. Methylation at Lys-42 and Lys-117 is necessary for the regulation of SOX2 proteasomal degradation.. Ubiquitinated by WWP2, leading to proteasomal degradation.

亚细胞定位

Nucleus

style
left-column

产品实验方案

style
left-column

靶点信息

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Binds to the proximal enhancer region of NANOG (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed : 18035408). Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). May function as a switch in neuronal development (By similarity).
See full target information SOX2
style
left-column
style
left-column
style
right-column

搭配使用产品

Proteins & Peptides

AB316492

Recombinant Human/Mouse Oct4 Protein Standard (His tag)

Sandwich ELISA - Recombinant Human/Mouse Oct4 Protein Standard (His tag) (AB316492)

  • 0
  • 0
Proteins & Peptides

AB316571

Recombinant Human KLF4 Protein Standard (His tag)

Sandwich ELISA - Recombinant Human KLF4 Protein Standard (His tag) (AB316571)

  • 0
  • 0
Primary Antibodies

AB97959

Anti-SOX2 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (AB97959)

  • 4
  • 75
Proteins & Peptides

AB134886

Recombinant human Nanog protein

  • 0
  • 0

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com