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生物活性等级

重组人Soluble TNF Receptor II蛋白(ab640)

Submit a review Q&A (1)References (1)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 98% SDS-PAGE
  • Endotoxin level: < 0.100 Eu/µg
  • Active: Yes
  • Suitable for: SDS-PAGE, Functional Studies

描述

  • 产品名称

    重组人Soluble TNF Receptor II蛋白
    参阅全部 Soluble TNF Receptor II 蛋白酶

  • 生物活性

    Determined by its inhibitory effect of the TNF-α mediated cytotoxicity in murine L-929 cells. The ED50 for this effect in the presence of 0.25 ng/ml of Recombinant Human TNF-α, is 0.125 μg/mL.

  • 纯度

    > 98 % SDS-PAGE.
    Sterile filtered. Greater than 98% pure by SDS-PAGE and HPLC analyses.

  • 内毒素水平

    < 0.100 Eu/µg

  • 表达系统

    Escherichia coli

  • Accession

    P20333

  • 蛋白长度

    Protein fragment

  • 无动物成分

    No

  • 性质

    Recombinant

    • 种属

      Human

    • 序列

      MAPEPGSTCR LREYYDQTAQ MCCSKCSPGQ HAKVFCTKTS DTVCDSCEDS TYTQLWNWVP ECLSCGSRCS SDQVETQACT REQNRICTCR PGWYCALSKQ EGCRLCAPLR KCRPGFGVAR PGTETSDVVC KPCAPGTFSN TTSSTDICRP HQICNVVAIP GNASMDAVCT STSP

    • 预测分子量

      19 kDa

技术指标

Our Abpromise guarantee covers the use of ab640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Functional Studies

  • 形式

    Lyophilized
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶
    Reconstitute by adding 200ul dH2O

常规信息

  • 别名

    • CD120b
    • p75
    • p75 TNF receptor
    • p75TNFR
    • p80 TNF alpha receptor
    • p80 TNF-alpha receptor
    • Soluble TNFR1B variant 1
    • TBP-2
    • TBPII
    • TNF R II
    • TNF R2
    • TNF R75
    • TNF-R2
    • TNF-RII
    • TNFBR
    • TNFR-II
    • TNFR1B
    • TNFR2
    • TNFR80
    • Tnfrsf1b
    • TNR1B_HUMAN
    • Tumor necrosis factor beta receptor
    • Tumor necrosis factor binding protein 2
    • Tumor necrosis factor receptor 2
    • Tumor necrosis factor receptor superfamily, member 1B
    • Tumor necrosis factor receptor type II
    • Tumor necrosis factor-binding protein 2
    see all

  • 功能

    Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.

  • 序列相似性

    Contains 4 TNFR-Cys repeats.

  • 翻译后修饰

    Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
    A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.

  • 细胞定位

    Secreted and Cell membrane.
  • Information by UniProt

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (1)

发表研究结果有使用 ab640？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab640 被引用在 1 文献中.

  • Dong Y  et al. Essential protective role of tumor necrosis factor receptor 2 in neurodegeneration. Proc Natl Acad Sci U S A 113:12304-12309 (2016). PubMed: 27791020

客户评价及客户问答

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Question

Kindly clarify why is there difference in molecular weight (80 kDa and 19 kDa)
for human TNF receptor II full length proteins with catalogue numbers ab132049
and ab640, respectively.

Read More
Answer


Ab640 is a fragment of the TNF Receptor II protein. It is comprised of amino acids 33-205 but is an active protein. The activity was determined by its inhibitory effect of the TNF-α mediated cytotoxicity in murine L-929 cells. The ED50 for this effect in the presence of 0.25 ng/ml of Recombinant Human TNF-α, is 0.125 μg/mL. This 172 amino acid protein runs around 19 kDa in a SDS-PAGE gel.


Ab132049, on the other hand, is a recombinant protein comprised of amino acids 23-461 plus a GST tag (25 kDa). The 80 kDa band size is the result of the 438 amino acid protein plus the 25 kDa tag. This protein has not been shown to have activity. It is only validated for ELISA, WB, and SDS-PAGE.


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