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AB93946

重组人 SOD2/MnSOD 蛋白 (His tag N-Terminus)

Recombinant human SOD2/MnSOD protein (His tag N-Terminus)

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(2 Publications)

Recombinant human SOD2/MnSOD protein (His tag N-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec, FuncS.

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SOD2

1 Images
SDS-PAGE - Recombinant human SOD2/MnSOD protein (His tag N-Terminus) (AB93946)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human SOD2/MnSOD protein (His tag N-Terminus) (AB93946)

15% SDS-PAGE analysis of ab93946 (3μg)

关键信息

纯度

>95% SDS-PAGE

ab93946 was purified using conventional chromatography techniques.

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

Mass Spec, FuncS, SDS-PAGE

applications

生物活性

Yes

生物学活性

Specific activity is > 1,200 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.8 at 25°C in a 1.5 ml reaction volume.

Activity Assay

  1. Prepare a 1.5 ml reaction mix into a suitable container and pre-chill on ice before use: The final concentrations are 50mM potassium phosphate, 0.1mM ethylendiaminetetraacetic acid, 0.01mM cytochrome C, 0.05mM xanthine, 0.005 units xanthine oxidase.
  2. Equilibrate to 25°C and monitor at A550nm until the value is constant using a spectrophotometer.
  3. Add 50 ul of recombinant SOD protein in various concentrations (0.5 ug, 1 ug) in assay buffer.
  4. Mix by inversion and record the increase at A550nm for 5 minutes.
访问

P04179

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

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搭配使用产品

Reagents, Controls & Accessories

AB234592

Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa) (AB234592)

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Proteins & Peptides

AB152558

Recombinant Human NDUFB8 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human NDUFB8 protein (GST tag N-Terminus) (AB152558)

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  • 0
Proteins & Peptides

AB224782

Recombinant human Triosephosphate isomerase protein (Active) (His tag N-Terminus)

SDS-PAGE - Recombinant human Triosephosphate isomerase protein (Active) (His tag N-Terminus) (AB224782)

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Primary Antibodies

AB68155

Anti-SOD2/MnSOD antibody [EPR2560Y]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (AB68155)

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  • 2
Proteins & Peptides

AB89248

Recombinant human ENO1 protein (Active) (Tag Free)

Bioactive

Western blot - Recombinant human ENO1 protein (Active) (Tag Free) (AB89248)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMKHSLPDLPYDYGALEPHINAQIMQLHHSKHHAAYVNNLNVTEEKYQEALAKGDVTAQIALQPALKFNGGGHINHSIFWTNLSPNGGGEPKGELLEAIKRDFGSFDKFKEKLTAASVGVQGSGWGWLGFNKERGHLQIAACPNQDPLQGTTGLIPLLGIDVWEHAYYLQYKNVRPDYLKAIWNVINWENVTERYMACKK","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P04179","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

形式
Liquid
运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
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常规信息

功能

Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.

序列相似性

Belongs to the iron/manganese superoxide dismutase family.

翻译后修饰

Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.. Acetylation at Lys-122 decreases enzymatic activity. Deacetylated by SIRT3 upon exposure to ionizing radiations or after long fasting (By similarity).. Polyubiquitinated; leading to proteasomal degradation. Deubiquitinated by USP36 which increases protein stability.

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产品实验方案

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靶点信息

Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
See full target information Superoxide dismutase [Mn], mitochondrial
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation 29:1769-1789 PubMed35301432

2022

A novel HIF-2α targeted inhibitor suppresses hypoxia-induced breast cancer stemness via SOD2-mtROS-PDI/GPR78-UPR axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Yan,Miao He,Lin Zhao,Huizhe Wu,Yanyun Zhao,Li Han,Binbin Wei,Dongman Ye,Xuemei Lv,Yan Wang,Weifan Yao,Haishan Zhao,Bo Chen,Zining Jin,Jian Wen,Yan Zhu,Tao Yu,Feng Jin,Minjie Wei

Free radical biology & medicine 108:595-609 PubMed28433661

2017

Differential protein acetylation assists import of excess SOD2 into mitochondria and mediates SOD2 aggregation associated with cardiac hypertrophy in the murine SOD2-tg heart.

Applications

Unspecified application

Species

Unspecified reactive species

Liwen Zhang,Chwen-Lih Chen,Patrick T Kang,Zhicheng Jin,Yeong-Renn Chen
View all publications
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