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AB81764

重组人 Smad4 蛋白 (His tag N-Terminus)

Recombinant Human Smad4 protein (His tag N-Terminus)

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(3 Publications)

Recombinant Human Smad4 protein (His tag N-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, WB, EMSA.

查看别名

DPC4, MADH4, SMAD4, SMAD family member 4, SMAD 4, hSMAD4, Deletion target in pancreatic carcinoma 4, Mothers against decapentaplegic homolog 4, MAD homolog 4, Mothers against DPP homolog 4

2 Images
Western blot - Recombinant Human Smad4 protein (His tag N-Terminus) (AB81764)
  • WB

Unknown

Western blot - Recombinant Human Smad4 protein (His tag N-Terminus) (AB81764)

All lanes:

Anti-Smad4 antibody (<a href='/products/unavailable/smad4-antibody-ab65217'>ab65217</a>) at 1/500 dilution

All lanes:

Western blot - Recombinant Human Smad4 protein (His tag N-Terminus) (ab81764) at 0.1 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution

true

Exposure time: 8min

SDS-PAGE - Recombinant Human Smad4 protein (His tag N-Terminus) (AB81764)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Smad4 protein (His tag N-Terminus) (AB81764)

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

关键信息

纯度

>90% SDS-PAGE

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

SDS-PAGE, EMSA, WB

applications

生物活性

No

访问

Q13485

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

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搭配使用产品

Reagents, Controls & Accessories

AB133411

Low Fluorescence Western Membrane (PVDF)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB40759

Anti-Smad4 antibody [EP618Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [EP618Y] (AB40759)

  • 4
  • 9
Proteins & Peptides

AB237007

Recombinant Human p53 protein

Mass Spectrometry - Recombinant Human p53 protein (AB237007)

  • 0
  • 0
Proteins & Peptides

AB95309

Recombinant Human MAX protein (His tag C-Terminus)

SDS-PAGE - Recombinant Human MAX protein (His tag C-Terminus) (AB95309)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "EMSA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMDNMSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPSSMMVKDEYVHDFEGQPSLSTEGHSIQTIQHPPSNRASTETYSTPALLAPSESNATSTANFPNIPVASTSQPASILGGSHSEGLLQIASGPQPGQQQNGFTGQPATYHHNSTTTWTGSRTAPYTPNLPHHQNGHLQHHPPMPPHPGHYWPVHNELAFQPPISNHPAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q13485","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

形式
Liquid
运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Smad4 also known as DPC4 or MADH4 is a central protein in the TGF-beta signaling pathway with a molecular mass of approximately 60 kDa. It acts as a signal transducer that facilitates communication from the cell surface to the nucleus. Smad4 is broadly expressed in various tissues playing an important role in the regulation of cellular processes. It forms a complex with receptor-regulated Smads (R-Smads) to translocate to the nucleus where it influences gene transcription.
Biological function summary

Smad4 influences cell proliferation differentiation and apoptosis by mediating signals from TGF-beta cytokines. It is part of the Smad protein family acting as a transcriptional controller. Upon TGF-beta receptor activation Smad4 forms complexes with Smad2 and Smad3 translocating to the nucleus to regulate genes imperative for cellular homeostasis. Its role in cell cycle regulation underlines its contribution to normal cellular functions and its potential involvement in disorders.

Pathways

Smad4 operates within the TGF-beta pathway linking extracellular signals to nuclear transcription alterations. It participates in the regulation of epithelial-mesenchymal transition (EMT) a process important for development and tumor progression. In these pathways Smad4 interacts closely with Smad2 and Smad3 orchestrating various cellular responses to external stimuli through transcriptional management.

Smad4 is highly related to cancer and juvenile polyposis syndrome. Mutations or deletions in Smad4 disrupt its function contributing to the progression of pancreatic cancer and colorectal cancer among others. Within these contexts Smad4 connects strongly to other proteins like p21 and cyclin-dependent kinase inhibitors which are important in cell cycle arrest and impede tumor growth.
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常规信息

功能

In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling (PubMed : 25514493). Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

序列相似性

Belongs to the dwarfin/SMAD family.

翻译后修饰

Phosphorylated by PDPK1.. Monoubiquitinated on Lys-519 by E3 ubiquitin-protein ligase TRIM33. Monoubiquitination hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade. Deubiquitination by USP9X restores its competence to mediate TGF-beta signaling.

亚细胞定位

Nucleus

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产品实验方案

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靶点信息

In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling (PubMed : 25514493). Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
See full target information SMAD4
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

eLife 11: PubMed35666955

2022

Species-specific sensitivity to TGFβ signaling and changes to the Mmp13 promoter underlie avian jaw development and evolution.

Applications

Unspecified application

Species

Unspecified reactive species

Spenser S Smith,Daniel Chu,Tiange Qu,Jessye A Aggleton,Richard A Schneider

Nature microbiology 4:339-351 PubMed30510168

2018

Antiviral activity of bone morphogenetic proteins and activins.

Applications

Unspecified application

Species

Unspecified reactive species

Lucy A Eddowes,Kinda Al-Hourani,Narayan Ramamurthy,Jamie Frankish,Hannah T Baddock,Cynthia Sandor,John D Ryan,Dahlene N Fusco,João Arezes,Eleni Giannoulatou,Sara Boninsegna,Stephane Chevaliez,Benjamin M J Owens,Chia Chi Sun,Paolo Fabris,Maria Teresa Giordani,Diego Martines,Slobodan Vukicevic,John Crowe,Herbert Y Lin,Jan Rehwinkel,Peter J McHugh,Marco Binder,Jodie L Babitt,Raymond T Chung,Matthew W Lawless,Andrew E Armitage,Caleb Webber,Paul Klenerman,Hal Drakesmith

Cancer research 71:234-44 PubMed21084277

2010

Phosphatase PRL-3 is a direct regulatory target of TGFbeta in colon cancer metastasis.

Applications

EMSA

Species

Unspecified reactive species

Yanjun Jiang,Xiao-Qiong Liu,Ashwani Rajput,Liying Geng,Melanie Ongchin,Qi Zeng,Gregory S Taylor,Jing Wang
View all publications
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