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AB177610

重组人 SKA1 蛋白 (His tag N-Terminus)

Recombinant Human SKA1 protein (His tag N-Terminus)

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Recombinant Human SKA1 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 255 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, Mass Spec.

查看别名

C18orf24, SKA1, SKA complex subunit 1, Spindle and kinetochore-associated protein 1

1 Images
SDS-PAGE - Recombinant Human SKA1 protein (His tag N-Terminus) (AB177610)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human SKA1 protein (His tag N-Terminus) (AB177610)

ab177610 (3ug) on a 15% SDS-PAGE.

关键信息

纯度

>80% SDS-PAGE

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

SDS-PAGE, Mass Spec

applications

生物活性

No

访问

Q96BD8

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMASSDLEQLCSHVNEKIGNIKKTLSLRNCGQEPTLKTVLNKIGDEIIVINELLNKLELEIQYQEQTNNSLKELCESLEEDYKDIEHLKENVPSHLPQVTVTQSCVKGSDLDPEEPIKVEEPEPVKKPPKEQRSIKEMPFITCDEFNGVPSYMKSRLTYNQINDVIKEINKAVISKYKILHQPKKSMNSVTRNLYHRFIDEETKDTKGRYFIVEADIKEFTTLKADKKFHVLLNILRHCRRLSEVRGGGLTRYVIT","proteinLength":"Full Length","predictedMolecularWeight":"31.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":255,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96BD8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Spindle and kinetochore associated protein 1 (SKA1) is part of a complex known for its essential role in the mitotic spindle checkpoint. SKA1 sometimes referred to by its alternate name Protein CASC5 has a molecular weight of approximately 9.5 kDa. SKA1 mainly expresses in tissues where cell division is active which include bone marrow and various cancerous tissues. Its function connects it to the assemblies of microtubules and kinetochores ensuring accurate chromosome segregation during mitosis.
Biological function summary

SKA1 interacts with other proteins to maintain the stability of the spindle microtubules attached to kinetochores. It forms the SKA complex with SKA2 and SKA3 which helps anchor microtubule fibers to kinetochores. This interaction is critical for tracking kinetochores along the microtubules during mitosis promoting proper chromosome alignment and segregation. The SKA complex ensures that daughter cells receive the correct number of chromosomes after cell division facilitating genomic integrity.

Pathways

SKA1 participates in the mechanisms governing mitosis and is involved in the progression through the cell cycle. It operates closely with the spindle assembly checkpoint pathway relying on interactions with NDC80 and SPC24 within the kinetochore complex. These interactions are necessary for monitoring chromosome alignment and preventing premature entry into anaphase. SKA1 supports the processes that control cell division fidelity making it vital to understanding normal cell cycle and division.

SKA1 associates strongly with various cancers such as breast and colorectal cancers. Abnormal expression or malfunction of SKA1 can lead to incorrect chromosome segregation contributing to aneuploidy a condition frequently seen in cancer cells. SKA1 interactions with proteins like MAD2 a mitotic checkpoint protein are implicated in tumorigenesis due to their roles in cell cycle control. Investigating SKA1 offers insights into potential therapeutic interventions especially targeting cell division in cancer treatment.
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特殊说明

形式

Liquid

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常规信息

功能

Component of the SKA complex, a microtubule plus end-binding complex of the outer kinetochore that stabilizes spindle microtubule-kinetochore attachments, promotes alignment of chromosomes at the mitotic spindle equator (chromosome congression) and assists suppression of the spindle assembly checkpoint (PubMed : 17093495, PubMed : 19289083, PubMed : 22371557, PubMed : 22483620, PubMed : 23085020, PubMed : 26981768, PubMed : 27697923, PubMed : 29487209, PubMed : 31804178). Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules (PubMed : 19289083, PubMed : 22483620, PubMed : 23085020, PubMed : 28479321, PubMed : 29487209). The outer kinetochore is made up of the ten-subunit KMN network complex, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components such as the SKA complex; together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle (PubMed : 17093495, PubMed : 19289083, PubMed : 23085020, PubMed : 28479321, PubMed : 29487209). The SKA complex is loaded onto bioriented kinetochores and it facilitates chromosome congression by stabilizing microtubules together with MAPRE1, and end-on attachment of the NDC80 complex to depolymerizing spindle microtubules, thereby assisting the poleward-moving kinetochore in withstanding microtubule pulling forces (PubMed : 19289083, PubMed : 22371557, PubMed : 22454517, PubMed : 23085020, PubMed : 24413531, PubMed : 27697923, PubMed : 28479321, PubMed : 28495837, PubMed : 29487209). The complex associates with dynamic microtubule plus-ends and can track both depolymerizing and elongating microtubules (PubMed : 23085020, PubMed : 29153323). The complex recruits protein phosphatase 1 (PP1) to the kinetochore in prometaphase and metaphase, to oppose spindle assembly checkpoint signaling and promote the onset of anaphase (PubMed : 26981768). In the complex, it mediates interactions with microtubules (PubMed : 19289083, PubMed : 22483620, PubMed : 23085020, PubMed : 24413531, PubMed : 27667719, PubMed : 29153323, PubMed : 36592928). It also stimulates AURKB/Aurora B catalytic activity (PubMed : 27697923). During meiosis the SKA complex stabilizes the meiotic spindle and is required for its migration to the cortex (By similarity).

序列相似性

Belongs to the SKA1 family.

翻译后修饰

Phosphorylated by AURKB at Thr-157 and Ser-242 which negatively regulates the association of the SKA complex with kinetochores to allow correction of aberrant kinetochore-microtubule interactions and promote mitotic sister chromatid biorientation.

亚细胞定位

Cytoskeleton

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产品实验方案

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靶点信息

Component of the SKA complex, a microtubule plus end-binding complex of the outer kinetochore that stabilizes spindle microtubule-kinetochore attachments, promotes alignment of chromosomes at the mitotic spindle equator (chromosome congression) and assists suppression of the spindle assembly checkpoint (PubMed : 17093495, PubMed : 19289083, PubMed : 22371557, PubMed : 22483620, PubMed : 23085020, PubMed : 26981768, PubMed : 27697923, PubMed : 29487209, PubMed : 31804178). Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules (PubMed : 19289083, PubMed : 22483620, PubMed : 23085020, PubMed : 28479321, PubMed : 29487209). The outer kinetochore is made up of the ten-subunit KMN network complex, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components such as the SKA complex; together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle (PubMed : 17093495, PubMed : 19289083, PubMed : 23085020, PubMed : 28479321, PubMed : 29487209). The SKA complex is loaded onto bioriented kinetochores and it facilitates chromosome congression by stabilizing microtubules together with MAPRE1, and end-on attachment of the NDC80 complex to depolymerizing spindle microtubules, thereby assisting the poleward-moving kinetochore in withstanding microtubule pulling forces (PubMed : 19289083, PubMed : 22371557, PubMed : 22454517, PubMed : 23085020, PubMed : 24413531, PubMed : 27697923, PubMed : 28479321, PubMed : 28495837, PubMed : 29487209). The complex associates with dynamic microtubule plus-ends and can track both depolymerizing and elongating microtubules (PubMed : 23085020, PubMed : 29153323). The complex recruits protein phosphatase 1 (PP1) to the kinetochore in prometaphase and metaphase, to oppose spindle assembly checkpoint signaling and promote the onset of anaphase (PubMed : 26981768). In the complex, it mediates interactions with microtubules (PubMed : 19289083, PubMed : 22483620, PubMed : 23085020, PubMed : 24413531, PubMed : 27667719, PubMed : 29153323, PubMed : 36592928). It also stimulates AURKB/Aurora B catalytic activity (PubMed : 27697923). During meiosis the SKA complex stabilizes the meiotic spindle and is required for its migration to the cortex (By similarity).
See full target information SKA1
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