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AB101133

重组 Human SIRT5 protein (GST tag N-Terminus)

Recombinant Human SIRT5 protein (GST tag N-Terminus)

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Recombinant Human SIRT5 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 310 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB.

查看别名

SIR2L5, SIRT5, Regulatory protein SIR2 homolog 5, SIR2-like protein 5

1 Images
SDS-PAGE - Recombinant Human SIRT5 protein (GST tag N-Terminus) (AB101133)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SIRT5 protein (GST tag N-Terminus) (AB101133)

SDS-PAGE showing ab101133 at approximately 60kDa.

关键信息

纯度

>95% SDS-PAGE

The purity was determined to be >95% by densitometry

表达系统

Escherichia coli

标签

GST tag N-Terminus

应用

SDS-PAGE, WB

applications

生物活性

No

访问

Q9NXA8

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.307% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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搭配使用产品

Primary Antibodies

AB259968

Anti-SIRT7 antibody [EPR23811-15]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunoprecipitation - Anti-SIRT7 antibody [EPR23811-15] (AB259968)

  • 0
  • 0
Primary Antibodies

AB115777

Anti-beta Actin antibody [SP124] - Cytoskeleton Marker

20ul selling size|RabMAb|Lab Essentials|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (AB115777)

  • 4
  • 1
Primary Antibodies

AB231137

Anti-SIRT4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT4 antibody (AB231137)

  • 1
  • 1
Primary Antibodies

AB259967

Anti-SIRT5 antibody [EPR23787-116]

20ul selling size|KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SIRT5 antibody [EPR23787-116] (AB259967)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"60 kDa","actualMolecularWeight":null,"aminoAcidEnd":310,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9NXA8","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

形式
Liquid
运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SIRT5 also known as Sirtuin 5 is a mitochondrial protein with enzymatic activity as a lysine desuccinylase demalonylase and deglutarylase. It has a molecular mass of approximately 34 kDa. This protein is expressed highly in tissues with high metabolic activity like the liver heart and brain where it participates in regulating energy metabolism. The activity of SIRT5 affects cellular processes by removing post-translational modifications from lysine residues modulating proteins' functions and interactions in these tissues.
Biological function summary

SIRT5 functions as an important regulator of mitochondrial metabolism and influences cellular energy homeostasis. It is not part of larger complexes but it interacts with various metabolic enzymes to control their function. Through its deacylase activity SIRT5 affects the urea cycle and fatty acid oxidation by modifying key enzymes involved in these processes. Its role in maintaining a balance in energy production and consumption highlights its importance in cellular metabolism.

Pathways

SIRT5 is involved in both the tricarboxylic acid (TCA) cycle and the regulation of reactive oxygen species (ROS). Within the TCA cycle SIRT5 desuccinylates and activates enzymes like citrate synthase enhancing the cycle's efficiency. In regulating ROS SIRT5 reduces oxidative stress by affecting enzymes such as superoxide dismutase 2 (SOD2). Its interaction with these pathways and proteins reflects its role in maintaining metabolic health and cellular integrity.

Alterations in SIRT5 levels have been connected to metabolic diseases such as obesity and neurodegenerative disorders like Alzheimer's disease. In obesity SIRT5 dysregulation can lead to inefficient metabolic processes and energy imbalance. In Alzheimer’s decreased SIRT5 activity may contribute to increased mitochondrial oxidative stress implicated in disease progression. These connections highlight SIRT5’s significance in both metabolic and neurodegenerative contexts linking with proteins like AMPK in obesity and tau proteins in Alzheimer's disease.
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常规信息

功能

NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed : 21908771, PubMed : 22076378, PubMed : 24703693, PubMed : 29180469). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting : acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed : 22076378, PubMed : 24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species (PubMed : 24140062). Activates SHMT2 by mediating its desuccinylation (PubMed : 29180469). Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (By similarity). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.

序列相似性

Belongs to the sirtuin family. Class III subfamily.

亚细胞定位

Mitochondrion matrix

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产品实验方案

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靶点信息

NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed : 21908771, PubMed : 22076378, PubMed : 24703693, PubMed : 29180469). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting : acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed : 22076378, PubMed : 24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species (PubMed : 24140062). Activates SHMT2 by mediating its desuccinylation (PubMed : 29180469). Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (By similarity). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.
See full target information SIRT5
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