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AB79949

重组人SIRT4蛋白

Recombinant Human SIRT4 protein

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Recombinant Human SIRT4 protein is a Human Fragment protein, in the 25 to 314 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

查看别名

SIR2L4, SIRT4, NAD-dependent ADP-ribosyltransferase sirtuin-4, NAD-dependent protein biotinylase sirtuin-4, NAD-dependent protein deacetylase sirtuin-4, Regulatory protein SIR2 homolog 4, SIR2-like protein 4

1 Images
SDS-PAGE - Recombinant Human SIRT4 protein (AB79949)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SIRT4 protein (AB79949)

10% SDS-PAGE, Coomassie staining.
Lane 1 : ab79949 8μg.
Lane 2 : Protein marker.

关键信息

纯度

>90% SDS-PAGE

表达系统

Escherichia coli

标签

GST tag N-Terminus

应用

SDS-PAGE

applications

生物活性

No

访问

Q9Y6E7

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.395% Tris HCl, 0.05% Sorbitan monolaurate, ethoxylated

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":314,"aminoAcidStart":25,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9Y6E7","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SIRT4 also known as Sirtuin 4 is a mitochondrial protein with a mass of approximately 36 kDa. It belongs to the sirtuin family of NAD+-dependent deacetylases. Unlike many other sirtuins SIRT4 does not function as a deacetylase; instead it exhibits ADP-ribosyltransferase activity. This activity regulates a range of mitochondrial functions. SIRT4 is expressed in various tissues including the liver pancreas and muscles where it plays different roles based on the tissue type.
Biological function summary

SIRT4 regulates energy metabolism and cellular stress responses. It plays key roles in the regulation of mitochondrial functions during fasting and impacts gluconeogenesis and fatty acid oxidation. SIRT4 inhibits glutamate dehydrogenase (GDH) affecting amino acid metabolism and insulin secretion. By acting in this capacity SIRT4 controls the flow of nutrients and energy homeostasis exhibiting its critical role in metabolic regulation.

Pathways

SIRT4 integrates into a broader metabolic network including the insulin signaling and AMPK pathways. It interacts with proteins like GDH and AMPK influencing mitochondrial energy output and stress response. This relationship places SIRT4 in an important position for maintaining energy balance and responding to cellular metabolic demands.

SIRT4 links to metabolic conditions such as diabetes and metabolic syndrome. Dysregulation of SIRT4 activity can lead to impaired insulin secretion and glucose intolerance. Furthermore SIRT4's interaction with other sirtuins particularly SIRT1 suggests a functional interplay in protecting against metabolic stress and possibly influencing the development of these metabolic disorders.
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特殊说明

形式

Liquid

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常规信息

功能

Acts as a NAD-dependent protein lipoamidase, biotinylase, deacetylase and ADP-ribosyl transferase (PubMed : 16959573, PubMed : 17715127, PubMed : 24052263, PubMed : 25525879). Catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications (PubMed : 24052263, PubMed : 25525879). Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner (PubMed : 25525879). Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity (PubMed : 16959573, PubMed : 17715127). Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1 : expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest (PubMed : 16959573, PubMed : 17715127). In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation (PubMed : 23663782). Acts as a tumor suppressor (PubMed : 23562301, PubMed : 23663782). Also acts as a NAD-dependent protein deacetylase : mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis (By similarity). Does not seem to deacetylate PC (PubMed : 23438705). Controls fatty acid oxidation by inhibiting PPARA transcriptional activation (PubMed : 24043310). Impairs SIRT1-PPARA interaction probably through the regulation of NAD(+) levels (PubMed : 24043310). Down-regulates insulin secretion (PubMed : 17715127).

序列相似性

Belongs to the sirtuin family. Class II subfamily.

亚细胞定位

Mitochondrion matrix

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Acts as a NAD-dependent protein lipoamidase, biotinylase, deacetylase and ADP-ribosyl transferase (PubMed : 16959573, PubMed : 17715127, PubMed : 24052263, PubMed : 25525879). Catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications (PubMed : 24052263, PubMed : 25525879). Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner (PubMed : 25525879). Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity (PubMed : 16959573, PubMed : 17715127). Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1 : expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest (PubMed : 16959573, PubMed : 17715127). In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation (PubMed : 23663782). Acts as a tumor suppressor (PubMed : 23562301, PubMed : 23663782). Also acts as a NAD-dependent protein deacetylase : mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis (By similarity). Does not seem to deacetylate PC (PubMed : 23438705). Controls fatty acid oxidation by inhibiting PPARA transcriptional activation (PubMed : 24043310). Impairs SIRT1-PPARA interaction probably through the regulation of NAD(+) levels (PubMed : 24043310). Down-regulates insulin secretion (PubMed : 17715127).
See full target information SIRT4
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关联产品

Select an associated product type
Alternative Product
Proteins & Peptides

AB101713

Recombinant Human SIRT4 protein

proteins-peptides

recombinant-human-sirt4-protein-ab101713

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