重组人SIRT3蛋白(Tagged) (ab125586)
Key features and details
- Expression system: Escherichia coli
- Purity: > 75% Densitometry
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组人SIRT3蛋白(Tagged)
参阅全部 SIRT3 蛋白酶 -
纯度
> 75 % Densitometry.
Purity was determined to be >75% by densitometry. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
分子量信息
Approx 66 kDa by SDS-PAGE -
氨基酸
1 to 399 -
标签
GST tag N-Terminus -
额外的序列信息
GenBank: NM_012239
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技术指标
Our Abpromise guarantee covers the use of ab125586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
常规信息
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别名
- hSIRT 3
- hSIRT3
- Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase
see all -
功能
NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the sirtuin family. Class I subfamily.
Contains 1 deacetylase sirtuin-type domain. -
翻译后修饰
Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity. -
细胞定位
Mitochondrion matrix. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human SIRT3 protein (ab125586)SDS Page analysis of ab125586 running at approximately 66 kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab125586 尚未被引用在任何文献中。