重组人SIRP alpha蛋白(His tag) (ab271743)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
描述
-
产品名称
重组人SIRP alpha蛋白(His tag)
参阅全部 SIRP alpha 蛋白酶 -
纯度
>= 90 % SDS-PAGE. -
表达系统
HEK 293 cells -
Accession
-
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
-
种属
Human -
序列
GVAGEEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGR ELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGNITPADAGTYYCVKFRK GSPDDVEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFS PRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQV ICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVR KFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDD VKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNER -
预测分子量
38 kDa including tags -
氨基酸
27 to 370 -
标签
His tag C-Terminus
-
相关产品
-
Related Products
技术指标
Our Abpromise guarantee covers the use of ab271743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
SDS-PAGE
-
形式
Liquid -
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)
常规信息
-
别名
- Signal regulatory protein alpha type 1
- Bit
- Brain Ig like molecule with tyrosine based activation motifs
see all -
功能
Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. -
组织特异性
Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. -
序列相似性
Contains 2 Ig-like C1-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻译后修饰
N-glycosylated.
Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11. -
细胞定位
Membrane. - Information by UniProt
图片
-
SDS-PAGE - Recombinant Human SIRP alpha protein (His tag) (ab271743)
SDS-PAGE analysis of 2.7 µg ab271743.
SIRP alpha is heavily glycosylated, resulting in apparent higher molecular weight.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab271743 尚未被引用在任何文献中。