重组人SIRP alpha蛋白(ab174006)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组人SIRP alpha蛋白
参阅全部 SIRP alpha 蛋白酶 -
生物活性
Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human SIRP alpha, His Tag (HPLC-verified) ab174006, Biotinylated Human CD47, Fc,Avitag ab246018, binding reactions. The half maximal inhibitory concentration (IC50) is 0.1245 μg/mL
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纯度
> 95 % SDS-PAGE. -
内毒素水平
< 1.000 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
EEELQVIQPD KSVLVAAGET ATLRCTATSL IPVGPIQWFR GAGPGRELIY NQKEGHFPRV TTVSDLTKRN NMDFSIRIGN ITPADAGTYY CVKFRKGSPD DVEFKSGAGT ELSVRAKPSA PVVSGPAARA TPQHTVSFTC ESHGFSPRDI TLKWFKNGNE LSDFQTNVDP VGESVSYSIH STAKVVLTRE DVHSQVICEV AHVTLQGDPL RGTANLSETI RVPPTLEVTQ QPVRAENQVN VTCQVRKFYP QRLQLTWLEN GNVSRTETAS TVTENKDGTY NWMSWLLVNV SAHRDDVKLT CQVEHDGQPA VSKSHDLKVS AHPKEQGSNT AAENTGSNER -
预测分子量
38 kDa including tags -
氨基酸
31 to 370 -
标签
His tag C-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab174006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C.
pH: 7.40
Constituents: 95% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
常规信息
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别名
- Signal regulatory protein alpha type 1
- Bit
- Brain Ig like molecule with tyrosine based activation motifs
see all -
功能
Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. -
组织特异性
Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. -
序列相似性
Contains 2 Ig-like C1-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻译后修饰
N-glycosylated.
Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11. -
细胞定位
Membrane. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human SIRP alpha protein (ab174006)
Reduced SDS-PAGE analysis of ab174006 staining overnight with Coomassie Blue.
DTT-reduced Protein migrates as 45-55 kDa due to glycosylation.
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Biological activity - Recombinant Human SIRP alpha protein (ab174006)FACS analysis shows that the binding of Human SIRP alpha, His Tag (HPLC-verified) (ab174006) to Jurkat expressing CD47 was inhibited by increasing concentration of neutralizing Anti-CD47 antibody. The concentration of SIRP alpha used is 1 μg/mL. IC50=0.2257 μg/mL
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Biological activity - Recombinant Human SIRP alpha protein (ab174006)FACS assay shows that Human SIRP alpha, His Tag (HPLC-verified) (ab174006) can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 1 μg/mL
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Biological activity - Recombinant Human SIRP alpha protein (ab174006)Immobilized Human CD47, Fc Tag (HPLC-verified) (ab220587) on AHC Biosensor, can bind Human SIRP alpha, His Tag (HPLC-verified) (ab174006) with an affinity constant of 0.72 μM as determined in BLI assay
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Biological activity - Recombinant Human SIRP alpha protein (ab174006)Loaded Human CD47, Mouse IgG2a Fc Tag, low endotoxin (ab220559) on Protein A Biosensor, can bind Human SIRP alpha, His Tag (HPLC-verified) (ab174006) with an affinity constant of 1.1 μM as determined in BLI assay
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Biological activity - Recombinant Human SIRP alpha protein (ab174006)Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human SIRP alpha, His Tag (HPLC-verified) ab174006, Biotinylated Human CD47, Fc,Avitag ab246018, binding reactions. The half maximal inhibitory concentration (IC50) is 0.1245 μg/mL
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HPLC - Recombinant Human SIRP alpha protein (ab174006)The purity of Human SIRP alpha, His Tag (HPLC-verified) ab174006 was greater than 90% as determined by SEC-HPLC.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab174006 尚未被引用在任何文献中。
