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AB288790

重组人SFRP4蛋白(Active)

Recombinant Human SFRP4 protein (Active)

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Recombinant Human SFRP4 protein (Active) is a Human Full Length protein, in the 19 to 346 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for FuncS.

查看别名

FRPHE, SFRP4, Secreted frizzled-related protein 4, sFRP-4, FrpHE

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Functional Studies - Recombinant Human SFRP4 protein (Active) (AB288790)
  • FuncS

Supplier Data

Functional Studies - Recombinant Human SFRP4 protein (Active) (AB288790)

Fully biologically active determined by the dose dependent inhibition of WNT-3a induced Wnt/ß-catenin activation in HEK293 TCF/LEF luciferase reporter cell line. ED50 is ≤ 52.4 ng/ml, corresponding to a specific activity of 1.9 x 10^4 units/mg.

Mass Spectrometry - Recombinant Human SFRP4 protein (Active) (AB288790)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human SFRP4 protein (Active) (AB288790)

Mass determination by ESI-TOF.

Predicted MW is 37888.18 Da. (+/- 10 Da by ESI-TOF). Observed MW is 37893.90 Da.

HPLC - Recombinant Human SFRP4 protein (Active) (AB288790)
  • HPLC

Supplier Data

HPLC - Recombinant Human SFRP4 protein (Active) (AB288790)

HPLC analysis of ab288790

SDS-PAGE - Recombinant Human SFRP4 protein (Active) (AB288790)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human SFRP4 protein (Active) (AB288790)

SDS-PAGE analysis of ab288790

关键信息

纯度

>95% HPLC

内毒素水平

<0.005 EU/µg

表达系统

HEK 293 cells

标签

Tag free

应用

FuncS

applications

生物活性

Yes

生物学活性

Fully biologically active determined by the dose dependent inhibition of WNT-3a induced Wnt/ß-catenin activation in HEK293 TCF/LEF luciferase reporter cell line. ED50 is ≤ 52.4 ng/ml, corresponding to a specific activity of 1.9 x 104 units/mg.

访问

Q6FHJ7

不含动物源

No

不含载体蛋白

No

种属

Human

复溶

Reconstitute in PBS

存储溶液

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"VRGAPCEAVRIPMCRHMPWNITRMPNHLHHSTQENAILAIEQYEELVDVNCSAVLRFFLCAMYAPICTLEFLHDPIKPCKSVCQRARDDCEPLMKMYNHSWPESLACDELPVYDRGVCISPEAIVTDLPEDVKWIDITPDMMVQERPLDVDCKRLSPDRCKCKKVKPTLATYLSKNYSYVIHAKIKAVQRSGCNEVTTVVDVKEIFKSSSPIPRTQVPLITNSSCQCPHILPHQDVLIMCYEWRSRMMLLENCLVEKWRDQLSKRSIQWEERLQEQRRTVQDKKKTAGRTSRSNPPKPKGKPPAPKPASPKKNIKTRSAQKRTNPKRV","proteinLength":"Full Length","predictedMolecularWeight":"40 kDa","actualMolecularWeight":null,"aminoAcidEnd":346,"aminoAcidStart":19,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q6FHJ7","tags":[]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SFRP4 also known as Secreted Frizzled Related Protein 4 is a 40 kDa protein involved in modulating Wnt signaling pathways. It is a member of the secreted frizzled-related proteins (sFRPs) that act as Wnt antagonists. SFRP4 has a cysteine-rich domain homologous to the Wnt-binding site on frizzled proteins. Expressed in many tissues including the heart lung and kidney SFRP4 plays a role in various physiological processes by influencing cell signaling.
Biological function summary

SFRP4 regulates cell growth and differentiation. It interacts with Wnt ligands preventing them from binding to their receptors. SFRP4 does not typically function as part of a protein complex acting instead as an antagonist on its own. By inhibiting Wnt activity SFRP4 influences embryonic development tissue homeostasis and even cancer progression. The impact of SFRP4 varies depending on the tissue-specific context reflecting its complex biological roles.

Pathways

SFRP4 is significant in the Wnt signaling pathway and the β-catenin independent pathway. By interacting with Wnt ligands SFRP4 can prevent the activation of canonical Wnt/β-catenin signaling a pathway involved in cell fate determination and stem cell renewal. It also influences the non-canonical pathways where the Wnt signaling does not involve β-catenin impacting processes like cell movement and polarity. In these pathways SFRP4 interacts with proteins such as Disheveled and Axin modulating their activities.

The disruption of SFRP4 expression links to osteoarthritis and breast cancer. Abnormal SFRP4 levels can result in improper Wnt signaling contributing to these conditions. In osteoarthritis SFRP4 imbalance affects cartilage integrity while in breast cancer altered SFRP4 expression may influence tumor growth and progression. Related proteins such as β-catenin in cancer or MMPs in osteoarthritis highlight the importance of SFRP4 in disease susceptibility and progression.
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特殊说明

形式

Lyophilized

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常规信息

功能

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types (By similarity). SFRP4 plays a role in bone morphogenesis. May also act as a regulator of adult uterine morphology and function. May also increase apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake (PubMed : 12952927).

序列相似性

Belongs to the secreted frizzled-related protein (sFRP) family.

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产品实验方案

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靶点信息

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types (By similarity). SFRP4 plays a role in bone morphogenesis. May also act as a regulator of adult uterine morphology and function. May also increase apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake (PubMed : 12952927).
See full target information SFRP4
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