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AB167960

重组人SET7蛋白

Recombinant human SET7 protein

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Recombinant human SET7 protein is a Human Full Length protein, in the 1 to 366 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, WB, FuncS.

查看别名

KIAA1717, KMT7, SET7, SET9, SETD7, Histone-lysine N-methyltransferase SETD7, Histone H3-K4 methyltransferase SETD7, Lysine N-methyltransferase 7, SET domain-containing protein 7, SET7/9, H3-K4-HMTase SETD7

2 Images
Functional Studies - Recombinant human SET7 protein (AB167960)
  • FuncS

Unknown

Functional Studies - Recombinant human SET7 protein (AB167960)

The specific activity of ab167960 was determined to be 40 nmol/min/mg as per activity assay protocol.

SDS-PAGE - Recombinant human SET7 protein (AB167960)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human SET7 protein (AB167960)

SDS Page analysis of ab167960

关键信息

纯度

>95% SDS-PAGE

表达系统

Baculovirus infected Sf9 cells

标签

GST tag N-Terminus

应用

SDS-PAGE, WB, FuncS

applications

生物活性

Yes

生物学活性

The specific activity of ab167960 was determined to be 40 nmol/min/mg as per activity assay protocol.

访问

Q8WTS6

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MDSDDEMVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQYKDNIRHGVCWIYYPDGGSLVGEVNEDGEMTGEKIAYVYPDERTALYGKFIDGEMIEGKLATLMSTEEGRPHFELMPGNSVYHFDKSTSSCISTNALLPDPYESERVYVAESLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVHPRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAFQATQQK","proteinLength":"Full Length","predictedMolecularWeight":"75 kDa","actualMolecularWeight":null,"aminoAcidEnd":366,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q8WTS6","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SET7 also known as SETD7 or KMT7 is a lysine methyltransferase that adds methyl groups to lysine residues on target proteins influencing their function and stability. This protein has a molecular mass of approximately 55 kDa. SET7 is expressed in a variety of tissues including the heart liver and pancreas suggesting its versatile role across different biological systems.
Biological function summary

SET7 contributes to gene regulation by methylating histone H3 at lysine 4 (H3K4) a mark generally associated with transcriptional activation. It also methylates non-histone proteins such as p53 TAF10 and STAT3 affecting their activity and interactions. SET7 operates distinctively and does not need to be part of any complex to fulfill its function effectively in diverse cellular processes.

Pathways

SET7 modulates transcriptional pathways such as the p53 signaling pathway and the NF-kB pathway. It interacts with the p53 protein affecting cell cycle regulation and the response to DNA damage. The modification of NF-kB by SET7 can influence inflammation and immune responses. Through these pathways SET7 plays a role in maintaining cellular homeostasis and response to stress.

SET7 is involved in conditions like cancer and cardiovascular diseases. Its methylation of p53 links it to tumor suppression activities while its impact on NF-kB relates to inflammatory responses. Dysregulation of SET7 activity can lead to abnormal cell proliferation and inflammation pointing to its potential as a target for therapeutic intervention.
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特殊说明

形式

Liquid

附加说明

Assessed by densitometry. Affinity purified.

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常规信息

功能

Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3 (PubMed : 11779497, PubMed : 11850410, PubMed : 12540855, PubMed : 12588998, PubMed : 16141209). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed : 12540855, PubMed : 12588998, PubMed : 16141209). Plays a central role in the transcriptional activation of genes such as collagenase or insulin (PubMed : 12588998, PubMed : 16141209). Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription (PubMed : 16141209). Has also methyltransferase activity toward non-histone proteins such as CGAS, p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins (PubMed : 15099517, PubMed : 15525938, PubMed : 16415881, PubMed : 35210392). Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II (PubMed : 15099517, PubMed : 16415881). Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation (PubMed : 15525938, PubMed : 16415881, PubMed : 17108971). Monomethylates 'Lys-491' of CGAS, promoting interaction between SGF29 and CGAS (By similarity).

序列相似性

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET7 subfamily.

亚细胞定位

Nucleus

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产品实验方案

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靶点信息

Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3 (PubMed : 11779497, PubMed : 11850410, PubMed : 12540855, PubMed : 12588998, PubMed : 16141209). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed : 12540855, PubMed : 12588998, PubMed : 16141209). Plays a central role in the transcriptional activation of genes such as collagenase or insulin (PubMed : 12588998, PubMed : 16141209). Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription (PubMed : 16141209). Has also methyltransferase activity toward non-histone proteins such as CGAS, p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins (PubMed : 15099517, PubMed : 15525938, PubMed : 16415881, PubMed : 35210392). Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II (PubMed : 15099517, PubMed : 16415881). Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation (PubMed : 15525938, PubMed : 16415881, PubMed : 17108971). Monomethylates 'Lys-491' of CGAS, promoting interaction between SGF29 and CGAS (By similarity).
See full target information SETD7
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