Key features and details
- Expression system: Escherichia coli
- Purity: >= 98% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies, WB
产品名称重组人Serum Amyloid A (mutated D60N + H71R)蛋白
参阅全部 Serum Amyloid A 蛋白酶
Tested by its ability to down- regulate lipid biosynthesis in aortic smooth muscle cells. The effective concentration was found to be 4 μM. Schreiber, BM. et al. Biochem J. 1999 Nov 15; 344 Pt 1:7-13
纯度>= 98 % SDS-PAGE.
>=98% by SDS-PAGE and HPLC analyses.
内毒素水平< 0.100 Eu/µg
蛋白长度Full length protein
序列MRSFFSFLGE AFDGARDMWR AYSDMREANY IGSDKYFHAR GNYDAAKRGP GGVWAAEAIS NARENIQRFF GRGAEDSLAD QAANEWGRSG KDPNHFRPAG LPEKY
修饰mutated D60N + H71R
Our Abpromise guarantee covers the use of ab50232 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
For most in vitro applications, apo-SAA exerts its biological activity in the concentration range of 10-50 µg/ml. ab50232 can be used as a WB positive control in conjunction with ab687.
Concentration information loading...
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
复溶Please reconstitute this product in water to a concentration of 1mg/ml.
- Amyloid fibrils
- SAA 2
- Serum amyloid A1 isoform 2
相关性Function: Major acute phase reactant. Apolipoprotein of the HDL complex. Tissue specificity: Expressed by the liver; secreted in plasma. Disease: Note=Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function. Note=Elevated serum SAA protein levels may be associated with lung cancer. Similarity: Belongs to the SAA family. PTM: This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end.
Anti-Serum Amyloid A antibody  (ab687) at 0.5 µg/ml +
Recombinant human Serum Amyloid A (mutated D60N + H71R) protein (ab50232) at 0.01 µg
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 minute
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.