重组人Serum Amyloid A (mutated D60N + H71R)蛋白(ab50232)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 98% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies, WB
描述
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产品名称
重组人Serum Amyloid A (mutated D60N + H71R)蛋白
参阅全部 Serum Amyloid A 蛋白酶 -
生物活性
Tested by its ability to down- regulate lipid biosynthesis in aortic smooth muscle cells. The effective concentration was found to be 4 μM. Schreiber, BM. et al. Biochem J. 1999 Nov 15; 344 Pt 1:7-13
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纯度
>= 98 % SDS-PAGE.
>=98% by SDS-PAGE and HPLC analyses. -
内毒素水平
< 0.100 Eu/µg -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MRSFFSFLGE AFDGARDMWR AYSDMREANY IGSDKYFHAR GNYDAAKRGP GGVWAAEAIS NARENIQRFF GRGAEDSLAD QAANEWGRSG KDPNHFRPAG LPEKY -
预测分子量
12 kDa -
修饰
mutated D60N + H71R
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技术指标
Our Abpromise guarantee covers the use of ab50232 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
Western blot
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形式
Lyophilized -
补充说明
For most in vitro applications, apo-SAA exerts its biological activity in the concentration range of 10-50 µg/ml. ab50232 can be used as a WB positive control in conjunction with ab687.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Please reconstitute this product in water to a concentration of 1mg/ml.
常规信息
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别名
- Amyloid fibrils
- SAA 2
- Serum amyloid A1 isoform 2
see all -
相关性
Function: Major acute phase reactant. Apolipoprotein of the HDL complex. Tissue specificity: Expressed by the liver; secreted in plasma. Disease: Note=Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function. Note=Elevated serum SAA protein levels may be associated with lung cancer. Similarity: Belongs to the SAA family. PTM: This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end.
图片
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Western blot - Recombinant human Serum Amyloid A protein (ab50232)Anti-Serum Amyloid A antibody [115] (ab687) at 0.5 µg/ml +
Recombinant human Serum Amyloid A (mutated D60N + H71R) protein (ab50232) at 0.01 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 minute
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab50232 尚未被引用在任何文献中。