重组人SENP8蛋白(ab109837)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS
描述
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产品名称
重组人SENP8蛋白
参阅全部 SENP8 蛋白酶 -
纯度
> 95 % SDS-PAGE.
No detergents such as urea, Triton or Tween were used to purify the proteins. Purified by using anion exchange chromatography (DEAE sepharose resin) and gel filtration chromatography (Sephacryl S 200) with 20mM Tris pH 7.5, 2mM EDTA. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MGSSHHHHHH SSGLVPRGSH MDPVVLSYMD SLLRQSDVSL LDPPSWLNDH IIGFAFEYFA NSQFHDCSDH VSFISPEVTQ FIKCTSNPAE IAMFLEPLDL PNKRVVFLAI NDNSNQAAGG THWSLLVYLQ DKNSFFHYDS HSRSNSVHAK QVAEKLEAFL GRKGDKLAFV EEKAPAQQNS YDCGMYVICN TEALCQNFFR QQTESLLQLL TPAYITKKRG EWKDLITTLA KK -
预测分子量
26 kDa -
氨基酸
1 to 212 -
标签
His tag N-Terminus
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相关产品
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Corresponding Antibody
技术指标
Our Abpromise guarantee covers the use of ab109837 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Mass Spectrometry
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质谱法
MALDI-TOF -
形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.02% DTT, 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
常规信息
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别名
- cysteine 2
- DEN 1
- DEN1
see all -
功能
Protease that catalyzes two essential functions in the NEDD8 pathway: processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as cullins or p53. -
组织特异性
Broadly expressed, with highest levels in kidney and pancreas. -
序列相似性
Belongs to the peptidase C48 family. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human SENP8 protein (ab109837)
3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab109837 被引用在 1 文献中.
- Shen LN et al. Structural basis of NEDD8 ubiquitin discrimination by the deNEDDylating enzyme NEDP1. EMBO J 24:1341-51 (2005). PubMed: 15775960