JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB87626

重组人 SAR1 蛋白 (His tag N-Terminus)

Recombinant Human SAR1 protein (His tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human SAR1 protein (His tag N-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

查看别名

SAR1, SARA, SARA1, SAR1A, Small COPII coat GTPase SAR1A, COPII-associated small GTPase, Secretion-associated Ras-related GTPase 1A

1 Images
SDS-PAGE - Recombinant Human SAR1 protein (His tag N-Terminus) (AB87626)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human SAR1 protein (His tag N-Terminus) (AB87626)

关键信息

纯度

>90% SDS-PAGE

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

SDS-PAGE

applications

生物活性

No

访问

Q9NR31

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

序列信息

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMSFIFEWIYNGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHSRLVESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIFGLYGQTTGKGNVTLKELNARPMEVFMCSVLKRQGYGEGFRWLSQYID","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9NR31","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SAR1 also known as SAR1A or SAR1B is a small GTPase belonging to the Ras superfamily. It plays an integral role in protein transport by regulating the formation of transport vesicles from the endoplasmic reticulum (ER). SAR1 has a molecular mass of about 21 kDa. It is ubiquitously expressed with significant levels observed in tissues involved in high protein synthesis activities such as liver and pancreas. Mechanically it switches between GDP-bound inactive and GTP-bound active states which is essential for its function in vesicle formation.
Biological function summary

SAR1 is an important component in the coat protein complex II (COPII) which is vital for ER-to-Golgi transport. Within this complex SAR1 assists in the initiation of vesicle budding by recruiting other COPII components such as Sec23/Sec24 and Sec13/Sec31. This assembly is important for maintaining efficient secretory pathways and cellular homeostasis. The COPII complex with SAR1 ensures the proper sorting and packaging of proteins destined for the Golgi apparatus.

Pathways

SAR1 plays an essential role in the secretory pathway facilitating intracellular protein trafficking. This pathway is vital for transporting proteins from the ER to the Golgi impacting processes such as cell signaling and membrane composition. SAR1 interacts with other proteins including Sec23 and Sec24 to ensure proper vesicle formation and function. These interactions highlight SAR1's role within the broader exocytic pathway signifying its importance in maintaining cellular function.

SAR1 mutations have been linked to lipid absorption disorders such as chylomicron retention disease (CMRD) and Anderson's disease. These conditions result from defective protein trafficking leading to impaired chylomicron secretion. In CMRD SAR1 dysfunction affects the related APOB protein contributing to the accumulation of fat in enterocytes and subsequent clinical symptoms. Understanding SAR1's function in these diseases may offer insights into therapeutic strategies targeting protein transport mechanisms.
style
left-column

特殊说明

形式

Liquid

附加说明

ab87626 is purified using conventional chromatography techniques.

style
left-column

常规信息

功能

Small GTPase that cycles between an active GTP-bound and an inactive GDP-bound state and mainly functions in vesicle-mediated endoplasmic reticulum (ER) to Golgi transport. The active GTP-bound form inserts into the endoplasmic reticulum membrane where it recruits the remainder of the coat protein complex II/COPII. The coat protein complex II assembling and polymerizing on endoplasmic reticulum membrane is responsible for both the sorting of cargos and the deformation and budding of membranes into vesicles destined to the Golgi (PubMed : 23433038, PubMed : 32358066, PubMed : 36369712). The GTPase activity of SAR1 by controlling the timing of COPII budding regulates the size of the formed vesicles and is important for cargo selection depending on their size (PubMed : 32358066). Together with SEC16A, forms the organized scaffold defining endoplasmic reticulum exit sites (ERES), some specific domains of the endoplasmic reticulum where COPII vesicles form (PubMed : 17005010). In addition to its role in vesicle trafficking, can also function as a leucine sensor regulating TORC1 signaling and more indirectly cellular metabolism, growth and survival. In absence of leucine, interacts with the GATOR2 complex via MIOS and inhibits TORC1 signaling. The binding of leucine abrogates the interaction with GATOR2 and the inhibition of the TORC1 signaling. This function is completely independent of the GTPase activity of SAR1B (PubMed : 34290409).

序列相似性

Belongs to the small GTPase superfamily. SAR1 family.

style
left-column

产品实验方案

style
left-column

靶点信息

Small GTPase that cycles between an active GTP-bound and an inactive GDP-bound state and mainly functions in vesicle-mediated endoplasmic reticulum (ER) to Golgi transport. The active GTP-bound form inserts into the endoplasmic reticulum membrane where it recruits the remainder of the coat protein complex II/COPII. The coat protein complex II assembling and polymerizing on endoplasmic reticulum membrane is responsible for both the sorting of cargos and the deformation and budding of membranes into vesicles destined to the Golgi (PubMed : 23433038, PubMed : 32358066, PubMed : 36369712). The GTPase activity of SAR1 by controlling the timing of COPII budding regulates the size of the formed vesicles and is important for cargo selection depending on their size (PubMed : 32358066). Together with SEC16A, forms the organized scaffold defining endoplasmic reticulum exit sites (ERES), some specific domains of the endoplasmic reticulum where COPII vesicles form (PubMed : 17005010). In addition to its role in vesicle trafficking, can also function as a leucine sensor regulating TORC1 signaling and more indirectly cellular metabolism, growth and survival. In absence of leucine, interacts with the GATOR2 complex via MIOS and inhibits TORC1 signaling. The binding of leucine abrogates the interaction with GATOR2 and the inhibition of the TORC1 signaling. This function is completely independent of the GTPase activity of SAR1B (PubMed : 34290409).
See full target information SAR1A
style
left-column
style
left-column
style
right-column

搭配使用产品

Primary Antibodies

AB125871

Anti-SAR1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SAR1 antibody (AB125871)

  • 0
  • 0
Proteins & Peptides

AB101214

Recombinant Human VAPB protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human VAPB protein (His tag N-Terminus) (AB101214)

  • 0
  • 0
Proteins & Peptides

AB99263

Recombinant Human Tryptophanyl tRNA synthetase/WRS protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Tryptophanyl tRNA synthetase/WRS protein (His tag N-Terminus) (AB99263)

  • 0
  • 0
Proteins & Peptides

AB101108

Recombinant Human TNIP1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human TNIP1 protein (His tag N-Terminus) (AB101108)

  • 0
  • 0

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com