JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB96772

重组人SAE1蛋白

Recombinant Human SAE1 protein

Be the first to review this product! Submit a review

|

(1 Publication)

Recombinant Human SAE1 protein is a Human protein, in the 1 to 346 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

查看别名

AOS1, SUA1, UBLE1A, SAE1, SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A

1 Images
SDS-PAGE - Recombinant Human SAE1 protein (AB96772)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SAE1 protein (AB96772)

15% SDS-PAGE showing ab96772 at approximately 42.2kDa (3μg).

关键信息

纯度

>90% SDS-PAGE

表达系统

Escherichia coli

标签

His tag N-Terminus T7 tag N-Terminus

应用

SDS-PAGE, Mass Spec

applications

生物活性

No

访问

Q9UBE0

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

序列信息

[{"sequence":"MHHHHHHMASMTGGQQMGRDLYDDDDKDRWGSMVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPESFFTQFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDYFLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRNDVLDSLGISPDLLPEDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGNGIVECLGPK","proteinLength":null,"predictedMolecularWeight":"42.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":346,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9UBE0","tags":[{"tag":"His","terminus":"N-Terminus"},{"tag":"T7","terminus":"N-Terminus"}]}]
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The protein SAE1 also known as SUMO-activating enzyme subunit 1 functions mechanically as a part of the SUMOylation process. It has a mass of approximately 39 kDa and is expressed in multiple cell types but is mainly found in the nucleus. SAE1 forms a heterodimeric structure with the protein SAE2 and together they are responsible for the initial step of SUMO activation which is important for SUMOylation a post-translational modification process.
Biological function summary

SAE1 is integral to numerous cellular processes that require precise regulation such as transcription DNA repair and signal transduction. It functions as part of the SUMO E1 enzyme complex which is essential for conjugating SUMO proteins to target substrates. This modification influences protein stability localization and interaction adding a layer of control in these cellular activities. The modulation of protein function by SAE1 impacts cell cycle control and stress responses indicating its breadth of influence across cellular mechanisms.

Pathways

The actions of SAE1 significantly impact the PI3K-Akt and DNA damage repair pathways. Its involvement in these pathways highlights its role in regulating cell survival and maintaining genomic integrity. SAE1's interaction within the SUMOylation machinery connects it to other proteins such as Ubc9 the E2 conjugating enzyme which highlights its importance in facilitating modifications necessary for a wide array of cellular functions and signaling cascades.

Defects or dysregulation of SAE1 have been linked to cancer and neurodegenerative conditions. Altered SUMOylation levels controlled by SAE1 can influence tumorigenesis through abnormal cell proliferation or impaired DNA damage responses connecting it with proteins like p53 and PARP1 which are critical in those pathways. Additionally SAE1's involvement in neurological disorders is associated with disrupted protein homeostasis linking it to pathologies where proper cellular regulatory mechanisms are compromised.
style
left-column

特殊说明

形式

Liquid

附加说明

ab96772 is purified using conventional chromatography techniques.

style
left-column

常规信息

功能

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

序列相似性

Belongs to the ubiquitin-activating E1 family.

亚细胞定位

Nucleus

style
left-column

产品实验方案

style
left-column

靶点信息

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
See full target information SAE1
style
left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Plant physiology 179:88-106 PubMed30333149

2018

The SUMO E3 Ligase MdSIZ1 Targets MdbHLH104 to Regulate Plasma Membrane H-ATPase Activity and Iron Homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Jie Zhou,Chun-Ling Zhang,Rui-Fen Zhang,Gui-Luan Wang,Yuan-Yuan Li,Yu-Jin Hao
View all publications
style
left-column
style
left-column
style
right-column

搭配使用产品

Primary Antibodies

AB185552

Anti-SAE1 antibody [EPR15398]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15398] (AB185552)

  • 5
  • 1
Proteins & Peptides

AB99263

Recombinant Human Tryptophanyl tRNA synthetase/WRS protein

SDS-PAGE - Recombinant Human Tryptophanyl tRNA synthetase/WRS protein (AB99263)

  • 0
  • 0
Proteins & Peptides

AB98233

Recombinant Human THTPA protein

SDS-PAGE - Recombinant Human THTPA protein (AB98233)

  • 0
  • 0
Proteins & Peptides

AB211951

Recombinant Human SUGT1 protein

  • 0
  • 0

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com