重组人RSK3蛋白(ab60882)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人RSK3蛋白 -
纯度
> 90 % SDS-PAGE.
Purity: >90% as determined by densitometry. Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human
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技术指标
Our Abpromise guarantee covers the use of ab60882 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
补充说明
ab204879 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- 90 kDa ribosomal protein S6 kinase 2
- HU 2
- KS6A2_HUMAN
see all -
功能
Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. May function as tumor suppressor in epithelial ovarian cancer cells. -
组织特异性
Widely expressed with higher expression in lung, skeletal muscle, brain, uterus, ovary, thyroid and prostate. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
翻译后修饰
Activated by phosphorylation at Ser-218 by PDPK1. Autophosphorylated on Ser-377, as part of the activation process. May be phosphorylated at Thr-356 and Ser-360 by MAPK1/ERK2 and MAPK3/ERK1.
N-terminal myristoylation results in an activated kinase in the absence of added growth factors. -
细胞定位
Nucleus. Cytoplasm. - Information by UniProt
图片
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Functional Studies - Recombinant human RSK3 protein (ab60882)The specific activity of RSK3 (ab60882) was determined to be 150 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human RSK3 protein (ab60882)SDS PAGE analysis of ab60882 -
SDS-PAGE - Recombinant human RSK3 protein (ab60882)SDS PAGE analysis of ab60882 -
Functional Studies - Recombinant human RSK3 protein (ab60882)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human RSK3 protein (ab60882)ab60882 on SDS-PAGE, MW ~112 kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab60882 尚未被引用在任何文献中。