重组人RSK1 p90蛋白(ab60880)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人RSK1 p90蛋白
参阅全部 RSK1 p90 蛋白酶 -
纯度
> 90 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPEFM PLAQLKEPWP LMELVPLDPE NGQTSGEEAG LQPSKDEGVL KEISITHHVK AGSEKADPSH FELLKVLGQG SFGKVFLVRK VTRPDSGHLY AMKVLKKATL KVRDRVRTKM ERDILADVNH PFVVKLHYAF QTEGKLYLIL DFLRGGDLFT RLSKEVMFTE EDVKFYLAEL ALGLDHLHSL GIIYRDLKPE NILLDEEGHI KLTDFGLSKE AIDHEKKAYS FCGTVEYMAP EVVNRQGHSH SADWWSYGVL MFEMLTGSLP FQGKDRKETM TLILKAKLGM PQFLSTEAQS LLRALFKRNP ANRLGSGPDG AEEIKRHVFY STIDWNKLYR REIKPPFKPA VAQPDDTFYF DTEFTSRTPK DSPGIPPSAG AHQLFRGFSF VATGLMEDDG KPRAPQAPLH SVVQQLHGKN LVFSDGYVVK ETIGVGSYSE CKRCVHKATN MEYAVKVIDK SKRDPSEEIE ILLRYGQHPN IITLKDVYDD GKHVYLVTEL MRGGELLDKI LRQKFFSERE ASFVLHTIGK TVEYLHSQGV VHRDLKPSNI LYVDESGNPE CLRICDFGFA KQLRAENGLL MTPCYTANFV APEVLKRQGY DEGCDIWSLG ILLYTMLAGY TPFANGPSDT PEEILTRIGS GKFTLSGGNW NTVSETAKDL VSKMLHVDPH QRLTAKQVLQ HPWVTQKDKL PQSQLSHQDL QLVKGAMAAT YSALNSSKPT PQLKPIESSI LAQRRVRKLP STTL -
标签
GST tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab60880 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
补充说明
ab204880 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- 90 kDa ribosomal protein S6 kinase 1
- dJ590P13.1
- dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1
see all -
功能
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
翻译后修饰
Autophosphorylated on Ser-380, as part of the activation process. - Information by UniProt
图片
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Functional Studies - Recombinant human RSK1 p90 protein (ab60880)The specific activity of RSK1 p90 (ab60880) was determined to be 210 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human RSK1 p90 protein (ab60880)SDS PAGE analysis of ab60880 -
SDS-PAGE - Recombinant human RSK1 p90 protein (ab60880)SDS PAGE analysis of ab60880 -
Functional Studies - Recombinant human RSK1 p90 protein (ab60880)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human RSK1 p90 protein (ab60880)ab60880 on SDS-PAGE, MW ~108 kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab60880 被引用在 1 文献中.
- Kroczynska B et al. Regulatory effects of ribosomal S6 kinase 1 (RSK1) in IFN{lambda} signaling. J Biol Chem 286:1147-56 (2011). PubMed: 21075852