重组人Rsk 2 / MAPKAP Kinase 1b蛋白(ab60881)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
-
产品名称
重组人Rsk 2 / MAPKAP Kinase 1b蛋白
参阅全部 Rsk 2 / MAPKAP Kinase 1b 蛋白酶 -
纯度
> 90 % SDS-PAGE.
Purity: >90% as determined by densitometry. Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
-
种属
Human
-
相关产品
-
Related Products
技术指标
Our Abpromise guarantee covers the use of ab60881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
SDS-PAGE
Functional Studies
-
形式
Liquid -
补充说明
ab204879 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity
-
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
-
别名
- 90 kDa ribosomal protein S6 kinase 3
- CLS
- HU 3
see all -
功能
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. -
组织特异性
Expressed in many tissues, highest levels in skeletal muscle. -
疾病相关
Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
翻译后修饰
Autophosphorylated on Ser-386, as part of the activation process.
Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369. - Information by UniProt
图片
-
Functional Studies - Recombinant human Rsk 2 / MAPKAP Kinase 1b protein (ab60881)The specific activity of Rsk 2 / MAPKAP Kinase 1b (ab60881) was determined to be 110 nmol/min/mg as per activity assay protocol and was equivalent to 157 nmol/min/mg as per radiometric assay
-
SDS-PAGE - Recombinant human Rsk 2 / MAPKAP Kinase 1b protein (ab60881)SDS PAGE analysis of ab60881 -
SDS-PAGE - Recombinant human Rsk 2 / MAPKAP Kinase 1b protein (ab60881)SDS PAGE analysis of ab60881 -
Functional Studies - Recombinant human Rsk 2 / MAPKAP Kinase 1b protein (ab60881)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human Rsk 2 / MAPKAP Kinase 1b protein (ab60881)ab60881 on SDS-PAGE, MW ~112 kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab60881 被引用在 1 文献中.
- Yang S & Counter CM Cell cycle regulated phosphorylation of the telomere-associated protein TIN2. PLoS One 8:e71697 (2013). PubMed: 23977114