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AB125828

重组人ROCK2蛋白

Recombinant human ROCK2 protein

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Recombinant human ROCK2 protein is a Human Fragment protein, in the 5 to 554 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, WB, FuncS.

查看别名

KIAA0619, ROCK2, Rho-associated protein kinase 2, Rho kinase 2, p164 ROCK-2, ROCK-II

2 Images
SDS-PAGE - Recombinant human ROCK2 protein (AB125828)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human ROCK2 protein (AB125828)

SDS-PAGE analysis of ab125828.

Functional Studies - Recombinant human ROCK2 protein (AB125828)
  • FuncS

Unknown

Functional Studies - Recombinant human ROCK2 protein (AB125828)

The specific activity of ab125828 was determined to be 605 nmol/min/mg.

关键信息

纯度

>95% Densitometry

表达系统

Baculovirus infected Sf9 cells

标签

His tag N-Terminus

应用

SDS-PAGE, FuncS, WB

applications

生物活性

Yes

生物学活性

The specific activity of ab125828 was determined to be 605 nmol/min/mg.

访问

O75116

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 0.71% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

ab204880 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity
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序列信息

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"66 kDa","actualMolecularWeight":null,"aminoAcidEnd":554,"aminoAcidStart":5,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"O75116","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
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特殊说明

形式

Liquid

附加说明

Purity was determined to be >95% by densitometry. Affinity purified.

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常规信息

功能

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.

序列相似性

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

翻译后修饰

Phosphorylation at Tyr-722 reduces its binding to RHOA and is crucial for focal adhesion dynamics. Dephosphorylation by PTPN11 stimulates its RHOA binding activity.. Cleaved by granzyme B during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.

亚细胞定位

Nucleus

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.
See full target information ROCK2
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