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AB153613

重组人 RNF168 蛋白 (GST tag N-Terminus)

Recombinant Human RNF168 protein (GST tag N-Terminus)

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Recombinant Human RNF168 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 571 aa range, expressed in Wheat germ, suitable for ELISA, WB.

查看别名

E3 ubiquitin-protein ligase RNF168, hRNF168, RING finger protein 168, RING-type E3 ubiquitin transferase RNF168, RNF168

1 Images
SDS-PAGE - Recombinant Human RNF168 protein (GST tag N-Terminus) (AB153613)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human RNF168 protein (GST tag N-Terminus) (AB153613)

ab153613 on a 12.5% SDS-PAGE stained with Coomassie Blue.

关键信息

表达系统

Wheat germ

标签

GST tag N-Terminus

应用

WB, ELISA

applications

生物活性

No

访问

Q8IYW5

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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搭配使用产品

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

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Proteins & Peptides

AB131927

Recombinant Human RNF8 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human RNF8 protein (GST tag N-Terminus) (AB131927)

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Primary Antibodies

AB283507

Anti-RNF168 antibody [EPR24239-2] - BSA and Azide free

KD Validated|RabMAb|Recombinant

Western blot - Anti-RNF168 antibody [EPR24239-2] - BSA and Azide free (AB283507)

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Assay Kits

AB282297

Mouse Prealbumin ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse Prealbumin ELISA Kit (AB282297)

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Assay Kits

AB230932

Mouse Apo B ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse Apo B ELISA Kit (AB230932)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"MALPKDAIPSLSECQCGICMEILVEPVTLPCNHTLCKPCFQSTVEKASLCCPFCRRRVSSWTRYHTRRNSLVNVELWTIIQKHYPRECKLRASGQESEEVADDYQPVRLLSKPGELRREYEEEISKVAAERRASEEEENKASEEYIQRLLAEEEEEEKRQAEKRRRAMEEQLKSDEELARKLSIDINNFCEGSISASPLNSRKSDPVTPKSEKKSKNKQRNTGDIQKYLTPKSQFGSASHSEAVQEVRKDSVSKDIDSSDRKSPTGQDTEIEDMPTLSPQISLGVGEQGADSSIESPMPWLCACGAEWYHEGNVKTRPSNHGKELCVLSHERPKTRVPYSKETAVMPCGRTESGCAPTSGVTQTNGNNTGETENEESCLLISKEISKRKNQESSFEAVKDPCFSAKRRKVSPESSPDQEETEINFTQKLIDLEHLLFERHKQEEQDRLLALQLQKEVDKEQMVPNRQKGSPDEYHLRATSSPPDKVLNGQRKNPKDGNFKRQTHTKHPTPERGSRDKNRQVSLKMQLKQSVNRRKMPNSTRDHCKVSKSAHSLQPSISQKSVFQMFQRCTK","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":571,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q8IYW5","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

形式
Liquid
纯化工艺
Proprietary technique
运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

RNF168 also known as Ring Finger Protein 168 is an E3 ubiquitin ligase with a molecular mass of approximately 63 kDa. It functions primarily in the DNA damage response by adding ubiquitin to histones at sites of double-strand breaks. RNF168 is expressed in various tissues but is notably active in cells that experience DNA damage. This protein structure contains a RING finger domain which facilitates its role in ubiquitination.
Biological function summary

The actions of RNF168 are critical in maintaining genome stability and it serves as a part of the DNA damage signaling complex. It collaborates with other proteins in recognizing DNA damage and signaling repair processes. RNF168 amplifies the ubiquitin signal which then attracts repair proteins to the site of damage. This function places it as an important component in the cellular response to DNA breaks.

Pathways

RNF168 is an important player in the DNA damage response pathway and is particularly associated with the homologous recombination repair mechanism. It works alongside proteins such as 53BP1 and BRCA1 to initiate repair processes. RNF168’s ubiquitination activity facilitates the recruitment of these repair proteins coordinating the cellular response to DNA lesions and ensuring effective repair.

RNF168 mutations or malfunctions have been linked to conditions like RIDDLE syndrome and certain cancers. In RIDDLE syndrome deficiencies in RNF168 disrupt the normal DNA repair processes leading to immunodeficiency radiosensitivity and learning difficulties. Cancer arises when the impaired DNA damage response fails to prevent mutations. Understanding RNF168 interactions particularly with proteins like 53BP1 and BRCA1 is essential for developing therapeutic strategies for these conditions.
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常规信息

功能

E3 ubiquitin-protein ligase required for accumulation of repair proteins to sites of DNA damage. Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination. Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and H2AX and amplifies the RNF8-dependent H2A ubiquitination, promoting the formation of 'Lys-63'-linked ubiquitin conjugates. This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of TP53BP1 and BRCA1. Also recruited at DNA interstrand cross-links (ICLs) sites and promotes accumulation of 'Lys-63'-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20/C1orf86 and Fanconi anemia (FA) complex, followed by interstrand cross-link repair. H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also involved in class switch recombination in immune system, via its role in regulation of DSBs repair. Following DNA damage, promotes the ubiquitination and degradation of JMJD2A/KDM4A in collaboration with RNF8, leading to unmask H4K20me2 mark and promote the recruitment of TP53BP1 at DNA damage sites. Not able to initiate 'Lys-63'-linked ubiquitination in vitro; possibly due to partial occlusion of the UBE2N/UBC13-binding region. Catalyzes monoubiquitination of 'Lys-13' and 'Lys-15' of nucleosomal histone H2A (H2AK13Ub and H2AK15Ub, respectively).

序列相似性

Belongs to the RNF168 family.

翻译后修饰

Sumoylated with SUMO1 by PIAS4 in response to double-strand breaks (DSBs).. Ubiquitinated.

亚细胞定位

Nucleus

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产品实验方案

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靶点信息

E3 ubiquitin-protein ligase required for accumulation of repair proteins to sites of DNA damage. Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination. Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and H2AX and amplifies the RNF8-dependent H2A ubiquitination, promoting the formation of 'Lys-63'-linked ubiquitin conjugates. This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of TP53BP1 and BRCA1. Also recruited at DNA interstrand cross-links (ICLs) sites and promotes accumulation of 'Lys-63'-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20/C1orf86 and Fanconi anemia (FA) complex, followed by interstrand cross-link repair. H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also involved in class switch recombination in immune system, via its role in regulation of DSBs repair. Following DNA damage, promotes the ubiquitination and degradation of JMJD2A/KDM4A in collaboration with RNF8, leading to unmask H4K20me2 mark and promote the recruitment of TP53BP1 at DNA damage sites. Not able to initiate 'Lys-63'-linked ubiquitination in vitro; possibly due to partial occlusion of the UBE2N/UBC13-binding region. Catalyzes monoubiquitination of 'Lys-13' and 'Lys-15' of nucleosomal histone H2A (H2AK13Ub and H2AK15Ub, respectively).
See full target information RNF168
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