重组人RNF146蛋白(ab153534)
Key features and details
- Expression system: Wheat germ
- Tags: GST tag N-Terminus
- Suitable for: ELISA, WB
描述
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产品名称
重组人RNF146蛋白 -
表达系统
Wheat germ -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MAGCGEIDHSINMLPTNRKANESCSNTAPSLTVPECAICLQTCVHPVSLP CKHVFCYLCVKGASWLGKRCALCRQEIPEDFLDKPTLLSPEELKAASRGN GEYAWYYEGRNGWWQYDERTSRELEDAFSKGKKNTEMLIAGFLYVADLEN MVQYRRNEHGRRRKIKRDIIDIPKKGVAGLRLDCDANTVNLARESSADGA DSVSAQSGASVQPLVSSVRPLTSVDGQLTSPATPSPDASTSLEDSFAHLQ LSGDNTAERSHRGEGEEDHESPSSGRVPAPDTSIEETESDASSDSEDVSA VVAQHSLTQQRLLVSNANQTVPDRSDRSGTDRSVAGGGTVSVSVRSRRPD GQCTVTEV -
氨基酸
1 to 358 -
标签
GST tag N-Terminus
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技术指标
Our Abpromise guarantee covers the use of ab153534 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
ELISA
Western blot
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
常规信息
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别名
- Dactylidin
- dJ351K20.1
- DKFZP434O1427
see all -
功能
E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated proteins and mediates their ubiquitination and subsequent degradation. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of poly-ADP-ribosylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate poly-ADP-ribosylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent poly-ADP-ribosylated proteins via its WWE domain and mediates their ubiquitination. -
组织特异性
Ubiquitously expressed. Up-regulated in brains from patients with Alzheimer disease. -
通路
Protein modification; protein ubiquitination. -
疾病相关
Note=Defects in RNF146 are a cause of susceptibility to breast cancer. -
序列相似性
Contains 1 RING-type zinc finger.
Contains 1 WWE domain. -
结构域
The WWE domain mediates non-covalent poly(ADP-ribose)-binding. -
翻译后修饰
Ubiquitinated; autoubiquitinated. Autoubiquitination is enhanced upon poly(ADP-ribose)-binding. -
细胞定位
Cytoplasm > cytosol. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human RNF146 protein (ab153534)ab153534 on a 12.5% SDS-PAGE stained with Coomassie Blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab153534 尚未被引用在任何文献中。
