重组人RIP3蛋白(Tagged) (ab125566)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
描述
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产品名称
重组人RIP3蛋白(Tagged)
参阅全部 RIP3 蛋白酶 -
生物活性
The specific activity of ab125566 was determined to be 38nmol/min/mg.
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纯度
> 70 % Densitometry.
Purity was determined to be >70% by densitometry. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
96 kDa including tags -
氨基酸
1 to 518 -
标签
GST tag N-Terminus
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相关产品
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab125566 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- Receptor interacting protein 3
- Receptor interacting serine threonine kinase 3
- Receptor interacting serine/threonine protein kinase 3
see all -
功能
Promotes apoptosis. -
组织特异性
Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers. -
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. -
细胞定位
Cytoplasm. - Information by UniProt
图片
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Functional Studies - Recombinant human RIP3 protein (Tagged) (ab125566)The specific activity of RIP3 (ab125566) was determined to be 10.6 nmol/min/mg as per activity assay protocol and was equivalent to 32 nmol/min/mg as per radiometric assay
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SDS-PAGE - Recombinant human RIP3 protein (Tagged) (ab125566)SDS PAGE analysis of ab125566 -
SDS-PAGE - Recombinant human RIP3 protein (Tagged) (ab125566)SDS PAGE analysis of ab125566 -
SDS-PAGE - Recombinant human RIP3 protein (Tagged) (ab125566)SDS-PAGE analysis of ab125566. -
Functional Studies - Recombinant human RIP3 protein (Tagged) (ab125566)The specific activity of ab125566 was determined to be 38 nmol/min/mg.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab125566 被引用在 2 文献中.
- Lamade AM et al. Inactivation of RIP3 kinase sensitizes to 15LOX/PEBP1-mediated ferroptotic death. Redox Biol 50:102232 (2022). PubMed: 35101798
- Choi SW et al. PELI1 Selectively Targets Kinase-Active RIP3 for Ubiquitylation-Dependent Proteasomal Degradation. Mol Cell 70:920-935.e7 (2018). PubMed: 29883609