重组人RIG-I/DDX58蛋白(Tagged) (ab271486)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Tags: DDDDK tag N-Terminus
- Suitable for: WB, SDS-PAGE
描述
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产品名称
重组人RIG-I/DDX58蛋白(Tagged)
参阅全部 RIG-I/DDX58 蛋白酶 -
纯度
>= 95 % SDS-PAGE. -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
108 kDa -
氨基酸
2 to 925 -
标签
DDDDK tag N-Terminus
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技术指标
Our Abpromise guarantee covers the use of ab271486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
100 µg/ml DDDDK peptide
常规信息
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别名
- Ddx58
- DDX58_HUMAN
- DEAD (Asp Glu Ala Asp) box polypeptide 58
see all -
功能
Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). Essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. -
组织特异性
Present in vascular smooth cells (at protein level). -
序列相似性
Belongs to the helicase family.
Contains 2 CARD domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
结构域
The repressor domain controls homomultimerization and interaction with MAVS.
The helicase domain is responsible for dsRNA recognition.
The 2 CARD domains are responsible for interaction with and signaling through MAVS.
The second CARD domain is the primary site for 'Lys-63'-linked ubiquitination. -
翻译后修饰
Isgylated. Conjugated to ubiquitin-like protein ISG15 upon IFN-beta stimulation.
Ubiquitinated. Undergoes 'Lys-63'-linked ubiquitination. Lys-172 is the critical site for TRIM25-mediated ubiquitination, for MAVS binding and to induce anti-viral signal transduction. Lys-154, Lys-164 and Lys-172 are critical sites for RNF135-mediated ubiquitination. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains. -
细胞定位
Cytoplasm. Colocalized with TRIM25 at cytoplasmic perinuclear bodies. - Information by UniProt
图片
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Western blot - Recombinant Human RIG-I/DDX58 protein (Tagged) (ab271486)
Western blot - Recombinant Human RIG-I/DDX58 protein (Tagged) (ab271486) used as a positive control.
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SDS-PAGE - Recombinant Human RIG-I/DDX58 protein (Tagged) (ab271486)SDS-PAGE analysis of ab271486.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab271486 被引用在 2 文献中.
- Si L et al. Self-assembling short immunostimulatory duplex RNAs with broad-spectrum antiviral activity. Mol Ther Nucleic Acids 29:923-940 (2022). PubMed: 36032397
- Si L et al. Self-assembling short immunostimulatory duplex RNAs with broad spectrum antiviral activity. bioRxiv N/A:N/A (2021). PubMed: 34845453