重组人RIG-I/DDX58蛋白(ab132502)
Key features and details
- Expression system: Wheat germ
- Suitable for: SDS-PAGE, WB, ELISA
描述
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产品名称
重组人RIG-I/DDX58蛋白
参阅全部 RIG-I/DDX58 蛋白酶 -
表达系统
Wheat germ -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MTTEQRRSLQAFQDYIRKTLDPTYILSYMAPWFREEEVQYIQAEKNNKGP MEAATLFLKFLLELQEEGWFRGFLDALDHAGYSGLYEAIESWDFKKIEKL EEYRLLLKRLQPEFKTRIIPTDIISDLSECLINQECEEILQICSTKGMMA GAEKLVECLLRSDKENWPKTLKLALEKERNKFSELWIVEKGIKDVETEDL EDKMETSDIQIFYQEDPECQNLSENSCPPSEVSDTNLYSPFKPRNYQLEL ALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKGKVVFFANQI PVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENNDIIILTPQI LVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLDQKLGGSSG PLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIATVKHNLEELE QVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRICKDLENLSQIQ NREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYND ALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELESV SRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGN PKLSFLKPGILTGRGKTNQNTGMTLPAQKCILDAFKASGDHNILIATSVA DEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIEK EQINMYKEKMMNDSILRLQTWDEAVFREKILHIQTHEKFIRDSQEKPKPV PDKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKECFVSRPHPKP KQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATG VQTLYSKWKDFHFEKIPFDPAEMSK -
预测分子量
133 kDa including tags -
氨基酸
1 to 925
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab132502 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Western blot
ELISA
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形式
Liquid -
补充说明
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
常规信息
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别名
- Ddx58
- DDX58_HUMAN
- DEAD (Asp Glu Ala Asp) box polypeptide 58
see all -
功能
Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). Essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. -
组织特异性
Present in vascular smooth cells (at protein level). -
序列相似性
Belongs to the helicase family.
Contains 2 CARD domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
结构域
The repressor domain controls homomultimerization and interaction with MAVS.
The helicase domain is responsible for dsRNA recognition.
The 2 CARD domains are responsible for interaction with and signaling through MAVS.
The second CARD domain is the primary site for 'Lys-63'-linked ubiquitination. -
翻译后修饰
Isgylated. Conjugated to ubiquitin-like protein ISG15 upon IFN-beta stimulation.
Ubiquitinated. Undergoes 'Lys-63'-linked ubiquitination. Lys-172 is the critical site for TRIM25-mediated ubiquitination, for MAVS binding and to induce anti-viral signal transduction. Lys-154, Lys-164 and Lys-172 are critical sites for RNF135-mediated ubiquitination. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains. -
细胞定位
Cytoplasm. Colocalized with TRIM25 at cytoplasmic perinuclear bodies. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human RIG-I/DDX58 protein (ab132502)12.5% SDS-PAGE analysis of ab132502 stained with Coomassie Blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab132502 尚未被引用在任何文献中。
