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AB167884

重组人RFXANK蛋白

Recombinant Human RFXANK protein

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Recombinant Human RFXANK protein is a Human Full Length protein, in the 1 to 237 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

查看别名

ANKRA1, RFXB, RFXANK, DNA-binding protein RFXANK, Ankyrin repeat family A protein 1, Regulatory factor X subunit B, Regulatory factor X-associated ankyrin-containing protein, RFX-B

1 Images
SDS-PAGE - Recombinant Human RFXANK protein (AB167884)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human RFXANK protein (AB167884)

SDS-PAGE of ab167884 (3?g) under reducing condition and visualized by coomassie blue stain.

关键信息

纯度

>85% SDS-PAGE

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

SDS-PAGE, Mass Spec

applications

生物活性

No

访问

O14593

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMELTQPAEDLIQTQQTPASELGDPEDPGEEAADGSDTVVLSLFPCTPEPVNPEPDASVSSPQGSSLKHSTTLTNRQRGNEVSALPATLDCDNLVNKPDERGFTPLIWASAFGEIETVRFLLEWGADPHILAKERESALSLASTGGYTDIVGLLLERDVDINIYDWNGGTPLLYAVRGNHVKCVEALLARGADLTTEADSGYTPMDLAVALGYRKVQQVIENHILKLFQSNLVPADPE","proteinLength":"Full Length","predictedMolecularWeight":"28 kDa","actualMolecularWeight":null,"aminoAcidEnd":237,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O14593","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The RFXANK protein also known as regulatory factor X-associated ankyrin-containing protein is a component of the RFX complex. RFXANK has a molecular mass of approximately 27 kDa. This protein plays a mechanical role in the regulation of transcription by interacting with DNA sequences and recruiting various co-factors necessary for gene expression. RFXANK is largely expressed in immune cells where it modulates processes important for immune function.
Biological function summary

RFXANK acts as part of the RFX complex which is known for its role in the regulation of class II major histocompatibility complex (MHC) genes. These genes are significant for proper immune response allowing immune cells to present antigens and initiate defense mechanisms. RFXANK through its interaction with other proteins like RFX5 and RFXAP stabilizes the DNA binding ability of the RFX complex enabling the transcription of genes essential for antigen presentation.

Pathways

The regulatory influence of RFXANK integrates into important immune pathways such as the MHC class II antigen presentation pathway. RFXANK's partnership with RFX5 and RFXAP facilitates binding to the X-box motif of MHC class II promoters ensuring the transcription initiation required for effective immune surveillance. These relations evidence RFXANK's vital place in immune system operations particularly in adaptive immunity.

RFXANK mutations or dysregulation relate to bare lymphocyte syndrome type II a severe immunodeficiency disorder. This condition arises when RFXANK fails to properly regulate MHC class II expression compromising antigen presentation and adaptive immune response. Connections exist between RFXANK and other disease-related proteins like RFX5 which highlight disruptions at the molecular level impacting immune functionality.
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特殊说明

形式

Liquid

附加说明

ab167884 is purified using conventional chromatography techniques.

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常规信息

功能

Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator/CIITA. May regulate other genes in the cell. RFX binds the X1 box of MHC-II promoters (PubMed : 10072068, PubMed : 10725724, PubMed : 9806546). May also potentiate the activation of RAF1 (By similarity).. Isoform 2 is not involved in the positive regulation of MHC class II genes.

翻译后修饰

Phosphorylated by RAF1.

亚细胞定位

Nucleus

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator/CIITA. May regulate other genes in the cell. RFX binds the X1 box of MHC-II promoters (PubMed : 10072068, PubMed : 10725724, PubMed : 9806546). May also potentiate the activation of RAF1 (By similarity).. Isoform 2 is not involved in the positive regulation of MHC class II genes.
See full target information RFXANK
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