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AB196086

重组人Repulsive Guidance Molecule A蛋白(His tag)

Recombinant Human Repulsive Guidance Molecule A protein (His tag)

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Recombinant Human Repulsive Guidance Molecule A protein (His tag) is a Human Fragment protein, in the 169 to 422 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC.

查看别名

RGM, RGMA, Repulsive guidance molecule A, RGM domain family member A

关键信息

纯度

>95% SDS-PAGE

内毒素水平

< 1 EU/µg

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

SDS-PAGE, HPLC

applications

生物活性

No

访问

Q96B86

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: PBS, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MNHKVHHHHHHMPHLRTFTDRFQTCKVQGAWPLIDNNYLNVQVTNTPVLPGSAATATSKLTIIFKNFQECVDQKVYQAEMDELPAAFVDGSKNGGDKHGANSLKITEKVSGQHVEIQAKYIGTTIVVRQVGRYLTFAVRMPEEVVNAVEDWDSQGLYLCLRGCPLNQQIDFQAFHTNAEGTGARRLAAASPAPTAPETFPYETAVAKCKEKLPVEDLYYQACVFDLLTTGDVNFTLAAYYALEDVKMLHSNKDKLHLYERTRDLPG","proteinLength":"Fragment","predictedMolecularWeight":"29.82 kDa","actualMolecularWeight":null,"aminoAcidEnd":422,"aminoAcidStart":169,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96B86","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Repulsive Guidance Molecule A (RGMa) also known as RGM domain family member A is a glycoprotein involved in axon guidance and neuronal differentiation. It has a molecular weight of approximately 37 kDa. RGMa is expressed broadly in the central nervous system particularly in developing nervous tissues. Beyond the CNS RGMa is also found in various non-neuronal tissues. Its role in axonal repulsion is facilitated by its interaction with the Neogenin receptor influencing the directionality and pathfinding of growing nerve fibers.
Biological function summary

RGMa plays a role in maintaining the structural organization of the nervous system by preventing aberrant nerve growth. RGMa forms part of a larger signaling complex that influences cell differentiation and migration. It acts by binding to Neogenin and recruiting co-receptors such as bone morphogenetic proteins (BMP) which regulate the differentiation and proliferation of neural progenitor cells. This interaction highlights its involvement in maintaining proper neural architecture and functionality.

Pathways

RGMa engages in the BMP signaling pathway and the axon guidance pathway. Its interaction with BMP proteins affects the Smad signaling cascade ultimately influencing gene expression essential for neuronal differentiation. Furthermore RGMa's influence on axon guidance involves coordination with other guidance cues that help establish neural circuitry during development. Its functional relationship with Neogenin and BMP signaling pathways highlights its regulatory role in neural patterning.

RGMa is linked to conditions such as spinal cord injury and multiple sclerosis. In the context of spinal cord injury increased expression of RGMa contributes to the inhibition of neural regeneration due to its repulsive signaling effects. It interacts with other proteins including Neogenin to exacerbate the inhibitory environment post-injury. In multiple sclerosis RGMa regulates immune cell infiltration into the CNS linking it to disease progression and pathology. Understanding RGMa's role opens potential therapeutic avenues to modulate its activity in these disorders.
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特殊说明

形式

Liquid

附加说明

The purity of ab196086 is greater than 95%, as determined by SEC-HPLC and reducing SDS-PAGE.

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常规信息

功能

Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.

序列相似性

Belongs to the repulsive guidance molecule (RGM) family.

翻译后修饰

Autocatalytically cleaved at low pH; the two chains remain linked via two disulfide bonds.

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产品实验方案

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靶点信息

Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.
See full target information RGMA
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搭配使用产品

Primary Antibodies

AB307652

Anti-Repulsive guidance molecule A antibody [EPR26279-90]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Repulsive guidance molecule A antibody [EPR26279-90] (AB307652)

  • 0
  • 0
Assay Kits

AB243687

Mouse RGMA ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse RGMA ELISA Kit (AB243687)

  • 0
  • 0
Proteins & Peptides

AB177643

Recombinant Human CD1E protein (denatured)

SDS-PAGE - Recombinant Human CD1E protein (denatured) (AB177643)

  • 0
  • 0
Proteins & Peptides

AB123188

Recombinant Human RPL8 protein

SDS-PAGE - Recombinant Human RPL8 protein (AB123188)

  • 0
  • 0

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