重组人Reptin/TIP49B/RUVB2蛋白(His tag) (ab225966)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组人Reptin/TIP49B/RUVB2蛋白(His tag) -
纯度
> 90 % SDS-PAGE. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
ATVTATTKVPEIRDVTRIERIGAHSHIRGLGLDDALEPRQASQGMVGQLA ARRAAGVVLEMIREGKIAGRAVLIAGQPGTGKTAIAMGMAQALGPDTPFT AIAGSEIFSLEMSKTEALTQAFRRSIGVRIKEETEIIEGEVVEIQIDRPA TGTGSKVGKLTLKTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGK ISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVIN SRTQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEV HMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSPHGIPIDLL DRLLIVSTTPYSEKDTKQILRIRCEEEDVEMSEDAYTVLTRIGLETSLRY AIQLITAASLVCRKRKGTEVQVDDIKRVYSLFLDESRSTQYMKEYQDAFL FNELKGETMDTS -
预测分子量
67 kDa including tags -
氨基酸
2 to 463 -
标签
His tag N-Terminus -
额外的序列信息
6xHis-SUMO tag at the N-terminus.
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab225966 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Constituents: 50% Glycerol (glycerin, glycerine), Tris buffer
常规信息
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别名
- 48 kDa TATA box-binding protein-interacting protein
- 48 kDa TBP-interacting protein
- 48-kDa TATA box-binding protein-interacting protein
see all -
功能
Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2. -
组织特异性
Ubiquitously expressed. Highly expressed in testis and thymus. -
序列相似性
Belongs to the ruvB family. -
结构域
The C-terminal domain is required for association with ATF2. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol. Although it is also present in the cytoplasm and associated with the cell membranes. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human Reptin/TIP49B/RUVB2 protein (His tag) (ab225966)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab225966 with 5% enrichment gel and 15% separation gel.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab225966 尚未被引用在任何文献中。