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AB225966

重组人Reptin/TIP49B/RUVB2蛋白(His tag)

Recombinant Human Reptin/TIP49B/RUVB2 protein (His tag)

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Recombinant Human Reptin/TIP49B/RUVB2 protein (His tag) is a Human Full Length protein, in the 2 to 463 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

查看别名

INO80J, TIP48, TIP49B, CGI-46, RUVBL2, RuvB-like 2, 48 kDa TATA box-binding protein-interacting protein, 51 kDa erythrocyte cytosolic protein, INO80 complex subunit J, Repressing pontin 52, TIP49b, TIP60-associated protein 54-beta, 48 kDa TBP-interacting protein, ECP-51, Reptin 52, TAP54-beta

1 Images
SDS-PAGE - Recombinant Human Reptin/TIP49B/RUVB2 protein (His tag) (AB225966)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Reptin/TIP49B/RUVB2 protein (His tag) (AB225966)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab225966 with 5% enrichment gel and 15% separation gel.

关键信息

纯度

>90% SDS-PAGE

表达系统

Escherichia coli

标签

6x His tag N-Terminus SUMO tag N-Terminus

应用

SDS-PAGE

applications

生物活性

No

访问

Q9Y230

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"ATVTATTKVPEIRDVTRIERIGAHSHIRGLGLDDALEPRQASQGMVGQLAARRAAGVVLEMIREGKIAGRAVLIAGQPGTGKTAIAMGMAQALGPDTPFTAIAGSEIFSLEMSKTEALTQAFRRSIGVRIKEETEIIEGEVVEIQIDRPATGTGSKVGKLTLKTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSRTQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSPHGIPIDLLDRLLIVSTTPYSEKDTKQILRIRCEEEDVEMSEDAYTVLTRIGLETSLRYAIQLITAASLVCRKRKGTEVQVDDIKRVYSLFLDESRSTQYMKEYQDAFLFNELKGETMDTS","proteinLength":"Full Length","predictedMolecularWeight":"67 kDa","actualMolecularWeight":null,"aminoAcidEnd":463,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9Y230","tags":[{"tag":"6x His","terminus":"N-Terminus"},{"tag":"SUMO","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Reptin also known as TIP49B or RUVB2 is an ATPase with a mass of approximately 52 kDa. It is expressed ubiquitously across various tissues playing significant roles in cellular processes. Mechanically Reptin functions as part of a complex that facilitates chromatin remodeling and transcription regulation. It often works in conjunction with its paralog Pontin/TIP49A/RUVB1 forming a dodecameric ring structure essential for its activity.
Biological function summary

Reptin plays essential roles in modulating gene expression and contributing to the assembly of ribonucleoprotein complexes. It forms part of the chromatin remodeling complexes such as INO80 and TIP60 participating in DNA repair processes and transcriptional regulation. Its function in these complexes makes it indispensable for maintaining genomic integrity and controlling cell cycle progression.

Pathways

Various critical cellular pathways involve Reptin's biological function. It is an important player in the Wnt/beta-catenin signaling pathway where it regulates the transcription of target genes. Additionally Reptin associates with the Myc pathway contributing to oncogenic transformation. In these pathways Reptin interacts with proteins like beta-catenin and c-Myc integrating signals necessary for cell proliferation and differentiation.

Aberrant Reptin activity links to cancer and neurological disorders. Overexpression of Reptin has strong connections to tumor progression and metastasis in cancers including colorectal and lung cancer. In these diseases Reptin partners with proteins such as c-Myc augmenting its transcriptional activity to promote cell growth. Additionally Reptin's dysregulation may influence neurodegenerative conditions by disrupting protein complexes involved in maintaining neural functions.
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特殊说明

形式

Liquid

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常规信息

功能

Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity (PubMed : 10428817, PubMed : 17157868, PubMed : 33205750). Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A (PubMed : 14966270). This modification may both alter nucleosome -DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription (PubMed : 14966270). This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair (PubMed : 14966270). The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400 (PubMed : 14966270). NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage (PubMed : 14966270). Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome (PubMed : 24463511). Proposed core component of the chromatin remodeling INO80 complex which exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding (PubMed : 16230350, PubMed : 21303910). Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex (PubMed : 10882073, PubMed : 16014379). May also inhibit the transcriptional activity of ATF2 (PubMed : 11713276). Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway where it negatively regulates expression of ER stress response genes (PubMed : 25652260). May play a role in regulating the composition of the U5 snRNP complex (PubMed : 28561026).

序列相似性

Belongs to the RuvB family.

亚细胞定位

Nucleus matrix

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产品实验方案

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靶点信息

Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity (PubMed : 10428817, PubMed : 17157868, PubMed : 33205750). Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A (PubMed : 14966270). This modification may both alter nucleosome -DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription (PubMed : 14966270). This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair (PubMed : 14966270). The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400 (PubMed : 14966270). NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage (PubMed : 14966270). Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome (PubMed : 24463511). Proposed core component of the chromatin remodeling INO80 complex which exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding (PubMed : 16230350, PubMed : 21303910). Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex (PubMed : 10882073, PubMed : 16014379). May also inhibit the transcriptional activity of ATF2 (PubMed : 11713276). Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway where it negatively regulates expression of ER stress response genes (PubMed : 25652260). May play a role in regulating the composition of the U5 snRNP complex (PubMed : 28561026).
See full target information RUVBL2
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搭配使用产品

Primary Antibodies

AB36569

Anti-Reptin/TIP49B/RUVB2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Reptin/TIP49B/RUVB2 antibody (AB36569)

  • 4
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Primary Antibodies

AB17968

Anti-BRD8/p120 antibody

Western blot - Anti-BRD8/p120 antibody (AB17968)

  • 0
  • 0
Proteins & Peptides

AB316468

Recombinant Mouse TGF beta Receptor II Protein Standard (His tag)

Sandwich ELISA - Recombinant Mouse TGF beta Receptor II Protein Standard (His tag) (AB316468)

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  • 0
Primary Antibodies

AB311880

Alexa Fluor® 555 Anti-ATM (phospho S1981) antibody [EP1890Y]

RabMAb|Recombinant

  • 0
  • 0

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