AB198157

重组人 RBBP4 + EED + SUZ12 + EZH2 蛋白 (His tag N-Terminus, DDDDK tag N-Terminus)

Name: 重组人RBBP4 + EED + SUZ12 + EZH2蛋白 (ab198157)| Abcam中文官网
Brand: Abcam Limited
SKU: AB198157
Price: 13350 CNY
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Recombinant human RBBP4 + EED + SUZ12 + EZH2 protein (His tag N-Terminus, DDDDK tag N-Terminus)

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Recombinant human RBBP4 + EED + SUZ12 + EZH2 protein (His tag N-Terminus, DDDDK tag N-Terminus) is a Human Full Length protein, in the 2 to 425 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS.

查看别名

CHET9, JJAZ1, KIAA0160, SUZ12, Polycomb protein SUZ12, Chromatin precipitated E2F target 9 protein, Joined to JAZF1 protein, Suppressor of zeste 12 protein homolog, ChET 9 protein

2 Images

Functional Studies - Recombinant human RBBP4 + EED + SUZ12 + EZH2 protein (His tag N-Terminus, DDDDK tag N-Terminus) (AB198157)

FuncS

Supplier Data

Functional Studies - Recombinant human RBBP4 + EED + SUZ12 + EZH2 protein (His tag N-Terminus, DDDDK tag N-Terminus) (AB198157)

Specific activity of ab198157 was 0.046 pmol/min/μg.

SDS-PAGE - Recombinant human RBBP4 + EED + SUZ12 + EZH2 protein (His tag N-Terminus, DDDDK tag N-Terminus) (AB198157)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human RBBP4 + EED + SUZ12 + EZH2 protein (His tag N-Terminus, DDDDK tag N-Terminus) (AB198157)

SDS PAGE analysis of ab198157.

关键信息

纯度

>95% SDS-PAGE

表达系统

Baculovirus infected Sf9 cells

应用

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学活性

50 μl reaction mix (50 mM Tris pH 8.8, 5 mM MgCl₂, 4 mM DTT, 20 μM S-adenosylmethionine, and 50-200 ng enzyme complex) add to the wells coated with the substrate. Incubate for 1 hour. Add antibody against methylated K27 residue of histone H3, incubate 1 hour. Then incubate 1 hour with the HRP-labeled secondary antibody, followed by addition of the HRP substrate to produce chemiluminescence.

访问

Q15022

不含动物源

不含载体蛋白

种属

Human

存储溶液

pH: 8 Preservative: 1.53% Imidazole Constituents: 10% Glycerol (glycerin, glycerine), 0.71% Tris HCl, 0.64% Sodium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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序列信息

[{"sequence":"ADKEAAFDDAVEERVINEEYKIWKKNTPFLYDLVMTHALEWPSLTAQWLPDVTRPEGKDFSIHRLVLGTHTSDEQNHLVIASVQLPNDDAQFDASHYDSEKGEFGGFGSVSGKIEIEIKINHEGEVNRARYMPQNPCIIATKTPSSDVLVFDYTKHPSKPDPSGECNPDLRLRGHQKEGYGLSWNPNLSGHLLSASDDHTICLWDISAVPKEGKVVDAKTIFTGHTAVVEDVSWHLLHESLFGSVADDQKLMIWDTRSNNTSKPSHSVDAHTAEVNCLSFNPYSEFILATGSADKTVALWDLRNLKLKLHSFESHKDEIFQVQWSPHNETILASSGTDRRLNVWDLSKIGEEQSPEDAEDGPPELLFIHGGHTAKISDFSWNPNEPWVICSVSEDNIMQVWQMAENIYNDEDPEGSVDPEGQGS","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":425,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q09028","tags":[{"tag":"His","terminus":"N-Terminus"}]},{"sequence":"GQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFSSNRQKILERTEILNQEWKQRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEKYKELTEQQLPGALPPECTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHRKCNYSFHATPNTYKRKNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTPPKRPGGRRRGRLPNNSSRPSTPTINVLESKDTDSDREAGTETGGENNDKEEEEKKDETSSSSEANSRCQTPIKMKPNIEPPENVEWSGAEASMFRVLIGTYYDNFCAIARLIGTKTCRQVYEFRVKESSIIAPAPAEDVDTPPRKKKRKHRLWAAHCRKIQLKKDGSSNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCGAADHWDSKNVSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREMEIP","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":751,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q15910","tags":[{"tag":"His","terminus":"N-Terminus"}]},{"sequence":"APQKHGGGGGGGSGPSAGSGGGGFGGSAAVAAATASGGKSGGGSCGGGGSYSASSSSSAAAAAGAAVLPVKKPKMEHVQADHELFLQAFEKPTQIYRFLRTRNLIAPIFLHRTLTYMSHRNSRTNIKRKTFKVDDMLSKVEKMKGEQESHSLSAHLQLTFTGFFHKNDKPSPNSENEQNSVTLEVLLVKVCHKKRKDVSCPIRQVPTGKKQVPLNPDLNQTKPGNFPSLAVSSNEFEPSNSHMVKSYSLLFRVTRPGRREFNGMINGETNENIDVNEELPARRKRNREDGEKTFVAQMTVFDKNRRLQLLDGEYEVAMQEMEECPISKKRATWETILDGKRLPPFETFSQGPTLQFTLRWTGETNDKSTAPIAKPLATRNSESLHQENKPGSVKPTQTIAVKESLTTDLQTRKEKDTPNENRQKLRIFYQFLYNNNTRQQTEARDDLHCPWCTLNCRKLYSLLKHLKLCHSRFIFNYVYHPKGARIDVSINECYDGSYAGNPQDIHRQPGFAFSRNGPVKRTPITHILVCRPKRTKASMSEFLESEDGEVEQQRTYSSGHNRLYFHSDTCLPLRPQEMEVDSEDEKDPEWLREKTITQIEEFSDVNEGEKEVMKLWNLHVMKHGFIADNQMNHACMLFVENYGQKIIKKNLCRNFMLHLVSMHDFNLISIMSIDKAVTKLREMQQKLEKGESASPANEEITEEQNGTANGFSEINSKEKALETDSVSGVSKQSKKQKL","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":739,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q15022","tags":[{"tag":"His","terminus":"N-Terminus"}]},{"sequence":"SEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTERPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSCENAIVCWKPGKMEDDIDKIKPSESNVTILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAKCTTLTHHKCGAAIRQTSFSRDSSILIAVCDDASIWRWDRLR","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":441,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"O75530","tags":[{"tag":"DDDDK","terminus":"N-Terminus"}]}]

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性能和储存信息

运输条件

Dry Ice

储存信息

Avoid freeze / thaw cycle

True

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特殊说明

形式

Liquid

附加说明

Affinity purified.

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常规信息

功能

Polycomb group (PcG) protein. Component of the PRC2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene (PubMed : 15225548, PubMed : 15231737, PubMed : 15385962, PubMed : 16618801, PubMed : 17344414, PubMed : 18285464, PubMed : 28229514, PubMed : 29499137, PubMed : 31959557). The PRC2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (PubMed : 12351676, PubMed : 12435631, PubMed : 15099518, PubMed : 15225548, PubMed : 15385962, PubMed : 15684044, PubMed : 16431907, PubMed : 18086877, PubMed : 18285464). Genes repressed by the PRC2 complex include HOXC8, HOXA9, MYT1 and CDKN2A (PubMed : 15231737, PubMed : 16618801, PubMed : 17200670, PubMed : 31959557).

序列相似性

Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.

翻译后修饰

Sumoylated, probably by PIAS2.

亚细胞定位

Nucleus

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information SUZ12

其他靶点

EED,RBBP4,EZH2

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重组人 RBBP4 + EED + SUZ12 + EZH2 蛋白 (His tag N-Terminus, DDDDK tag N-Terminus)

Recombinant human RBBP4 + EED + SUZ12 + EZH2 protein (His tag N-Terminus, DDDDK tag N-Terminus)

Functional Studies - Recombinant human RBBP4 + EED + SUZ12 + EZH2 protein (His tag N-Terminus, DDDDK tag N-Terminus) (AB198157)

SDS-PAGE - Recombinant human RBBP4 + EED + SUZ12 + EZH2 protein (His tag N-Terminus, DDDDK tag N-Terminus) (AB198157)

关键信息

纯度

表达系统

标签

应用

生物活性

生物学活性

访问

不含动物源

不含载体蛋白

种属

存储溶液