AB268916

重组人RanGAP1蛋白(His tag)

Name: 重组人RanGAP1蛋白(His tag) (ab268916)| Abcam中文官网
Brand: Abcam Limited
SKU: AB268916
Price: 2893 CNY
Availability: InStock

Recombinant Human RanGAP1 protein (His tag)

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Recombinant Human RanGAP1 protein (His tag) is a Human Fragment protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

查看别名

KIAA1835, SD, RANGAP1, Ran GTPase-activating protein 1, RanGAP1

1 Images

SDS-PAGE - Recombinant Human RanGAP1 protein (His tag) (AB268916)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human RanGAP1 protein (His tag) (AB268916)

SDS-PAGE analysis of ab268916

关键信息

纯度

>90% SDS-PAGE

表达系统

Escherichia coli

应用

SDS-PAGE

applications

生物活性

访问

P46060

不含动物源

不含载体蛋白

种属

Human

存储溶液

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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序列信息

[{"sequence":"418-end","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P46060","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

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性能和储存信息

运输条件

Dry Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

False

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

RanGAP1 also known as Ran GTPase-activating protein 1 plays an important role in regulating nucleocytoplasmic transport. Mechanically it converts RanGTP to RanGDP in the cytoplasm which is essential for the recycling of Ran and maintenance of the Ran gradient across the nuclear envelope. The molecular mass of RanGAP1 is approximately 70 kDa. RanGAP1 is expressed in various cell types with significant presence in the cytoplasm due to its function.

Biological function summary

Many cellular processes rely on the action of RanGAP1 as it forms part of the Ran cycle. This protein is an integral component of the nuclear transport complex which regulates the bidirectional movement of RNA and proteins through the nuclear pores. Through its action RanGAP1 indirectly influences nuclear import and export playing an essential part in cell cycle regulation and mitosis.

Pathways

Several key cellular pathways are dependent on RanGAP1 activity. For instance it is a component of the mitotic spindle assembly pathway and also plays a role in the RNA processing pathway. In these pathways RanGAP1 works closely with other proteins such as RanBP1 and RCC1 facilitating important cellular events that require strict regulation of nuclear-cytoplasmic transport.

Disruptions in RanGAP1 function have been associated with certain types of cancer and neurodegenerative diseases. Aberrant regulation of RanGAP1 activity can lead to improper cell cycle progression contributing to oncogenesis. Furthermore changes in the expression of RanGAP1 and its interacting proteins like RanBP2 may relate to disorders like Huntington's disease where cellular transport mechanisms are compromised.

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特殊说明

形式

Liquid

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常规信息

功能

GTPase activator for RAN (PubMed : 16428860, PubMed : 8146159, PubMed : 8896452). Converts cytoplasmic GTP-bound RAN to GDP-bound RAN, which is essential for RAN-mediated nuclear import and export (PubMed : 27160050, PubMed : 8896452). Mediates dissociation of cargo from nuclear export complexes containing XPO1, RAN and RANBP2 after nuclear export (PubMed : 27160050).

序列相似性

Belongs to the RNA1 family.

翻译后修饰

Phosphorylation occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBE2I, on nuclear pore complex (NPC) disassembly and during mitosis.. Sumoylated (PubMed:11854305, PubMed:15037602, PubMed:26304119, PubMed:27160050). Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis (PubMed:11854305). Also required for interaction with RANBP2 and is mediated by UBE2I (PubMed:27160050). Desumoylated by HINT1 (By similarity).

亚细胞定位

Nucleus

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information RANGAP1

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重组人RanGAP1蛋白(His tag)

Recombinant Human RanGAP1 protein (His tag)

SDS-PAGE - Recombinant Human RanGAP1 protein (His tag) (AB268916)

关键信息

纯度

表达系统

标签

应用

生物活性

访问

不含动物源

不含载体蛋白

种属

存储溶液