重组人Rad51蛋白(Active) (ab63808)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, WB
描述
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产品名称
重组人Rad51蛋白(Active)
参阅全部 Rad51 蛋白酶 -
生物活性
Retain nuclear filament forming and strand-exchange activity as well as interaction with Rad52.
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纯度
> 95 % SDS-PAGE.
Highly purified by several steps of chromatography. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
GSHMAMQMQL EANADTSVEE ESFGPQPISR LEQCGINAND VKKLEEAGFH TVEAVAYAPK KELINIKGIS EAKADKILAE AAKLVPMGFT TATEFHQRRS EIIQITTGSK ELDKLLQGGI ETGSITEMFG EFRTGKTQIC HTLAVTCQLP IDRGGGEGKA MYIDTEGTFR PERLLAVAER YGLSGSDVLD NVAYARAFNT DHQTQLLYQA SAMMVESRYA LLIVDSATAL YRTDYSGRGE LSARQMHLAR FLRMLLRLAD EFGVAVVITN QVVAQVDGAA MFAADPKKPI GGNIIAHAST TRLYLRKGRG ETRICKIYDS PCLPEAEAMF AINADGVGDA KD -
预测分子量
37 kDa -
氨基酸
1 to 339 -
额外的序列信息
Gly Ser His at the N-terminal
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技术指标
Our Abpromise guarantee covers the use of ab63808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
Western blot
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形式
Liquid -
补充说明
This protein can be used for:
1) Studies on homologous recombination mechanism and SOS response.
2) Useful in the screening using probe from library by promotion of DNA hybridization.
3) Facilitate DNA observation by electron microscope due to nucleofilament formation with DNA.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 6
Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.0146% EDTA, 10% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- BRCA1/BRCA2 containing complex, subunit 5
- BRCC 5
- BRCC5
see all -
功能
Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. -
组织特异性
Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast. -
疾病相关
Breast cancer
Mirror movements 2
Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers. -
序列相似性
Belongs to the RecA family. RAD51 subfamily.
Contains 1 HhH domain. -
结构域
The nuclear localization may reside in the C-terminus (between 259 and 339 AA). -
翻译后修饰
Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function. -
细胞定位
Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner. - Information by UniProt
图片
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SDS-PAGE - Recombinant human Rad51 protein (ab63808)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab63808 被引用在 7 文献中.
- Garza S & Paik R CRISPR/Cas9 Endonuclease-Mediated Mouse Genome Editing of One-Cell and/or Two-Cell Embryos by Electroporation, and the Use of Rad51 to Enhance Knock-In Allele Homozygosity via Interhomolog Repair Mechanism. Methods Mol Biol 2631:253-266 (2023). PubMed: 36995671
- Blay V et al. Comparison of ATP-binding pockets and discovery of homologous recombination inhibitors. Bioorg Med Chem 70:116923 (2022). PubMed: 35841829
- Yadav T et al. TERRA and RAD51AP1 promote alternative lengthening of telomeres through an R- to D-loop switch. Mol Cell 82:3985-4000.e4 (2022). PubMed: 36265486
- Ouyang J et al. RNA transcripts stimulate homologous recombination by forming DR-loops. Nature 594:283-288 (2021). PubMed: 33981036
- Wilde JJ et al. Efficient embryonic homozygous gene conversion via RAD51-enhanced interhomolog repair. Cell 184:3267-3280.e18 (2021). PubMed: 34043941
- Roy DS et al. Anterior thalamic dysfunction underlies cognitive deficits in a subset of neuropsychiatric disease models. Neuron 109:2590-2603.e13 (2021). PubMed: 34197733
- Daza-Martin M et al. Isomerization of BRCA1-BARD1 promotes replication fork protection. Nature 571:521-527 (2019). PubMed: 31270457