AB97946

重组人Rad1蛋白

Name: 重组人Rad1蛋白 (ab97946)| Abcam中文官网
Brand: Abcam Limited
SKU: AB97946
Price: 4822 CNY
Availability: InStock

Recombinant Human Rad1 protein

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Recombinant Human Rad1 protein is a Human Full Length protein, in the 1 to 282 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

查看别名

REC1, RAD1, Cell cycle checkpoint protein RAD1, hRAD1, Rad1-like DNA damage checkpoint protein

1 Images

SDS-PAGE - Recombinant Human Rad1 protein (AB97946)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Rad1 protein (AB97946)

15% SDS-PAGE showing ab97946 at approximately 33.9kDa (3μg).

关键信息

纯度

>85% SDS-PAGE

表达系统

Escherichia coli

应用

Mass Spec, SDS-PAGE

applications

生物活性

访问

O60671

不含动物源

不含载体蛋白

种属

Human

存储溶液

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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序列信息

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMPLLTQQIQDEDDQYSLVASLDNVRNLSTILKAIHFREHATCFATKNGIKVTVENAKCVQANAFIQAGIFQEFKVQEESVTFRINLTVLLDCLSIFGSSPMPGTLTALRMCYQGYGYPLMLFLEEGGVVTVCKINTQEPEETLDFDFCSTNVINKIILQSEGLREAFSELDMTSEVLQITMSPDKPYFRLSTFGNAGSSHLDYPKDSDLMEAFHCNQTQVNRYKISLLKPSTKALVLSCKVSIRTDNRGFLSLQYMIRNEDGQICFVEYYCCPDEEVPESES","proteinLength":"Full Length","predictedMolecularWeight":"33.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":282,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O60671","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

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性能和储存信息

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

False

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Rad1 also known as RAD1 homolog 1 from Saccharomyces cerevisiae functions mechanically as an exonuclease involved in DNA repair processes. It forms a part of the 9-1-1 checkpoint complex which acts as a sensor for DNA damage. Rad1 has a molecular mass of approximately 34 kDa and is expressed in various tissues where cell division occurs. Its primary role is in maintaining genomic stability by participating in the recognition and repair of abnormal DNA structures.

Biological function summary

Rad1 plays an essential role in the DNA damage response by being part of the 9-1-1 complex which includes Rad9 and Hus1 proteins. This complex is an important player in activating the ATR signaling pathway which helps cells respond to DNA replication stress and damage. Rad1 helps ensure proper checkpoint activation preventing cells with damaged DNA from progressing through the cell cycle which further safeguards genomic integrity.

Pathways

The protein Rad1 functions critically within the ATR-Chk1 signaling pathway a significant route for controlling cell cycle checkpoints. In this context Rad1 associates with proteins such as ATR and Chk1 facilitating the signaling necessary for cell cycle arrest under conditions of DNA stress. Rad1 also interacts with the base excision repair pathway where it works with other repair proteins to rectify oxidative DNA damage and maintain cellular viability.

Rad1 has a connection with cancer due to its involvement in DNA repair pathways. Impaired Rad1 function can lead to accumulation of DNA damage contributing to carcinogenesis. Additionally Rad1’s participation in maintaining genomic stability links it to disorders like Fanconi anemia where DNA repair defects are evident. In these contexts Rad1's interactions with proteins like BRCA1 further highlight its importance in protective cellular mechanisms.

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特殊说明

形式

Liquid

附加说明

ab97946 is purified using conventional chromatography techniques.

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常规信息

功能

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair (PubMed : 10846170, PubMed : 10884395). The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex (PubMed : 12578958). Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER) (PubMed : 15871698). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates (PubMed : 15314187, PubMed : 15556996, PubMed : 15871698). The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase (PubMed : 21659603).

序列相似性

Belongs to the rad1 family.

亚细胞定位

Nucleus

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information RAD1

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重组人Rad1蛋白

Recombinant Human Rad1 protein

SDS-PAGE - Recombinant Human Rad1 protein (AB97946)

关键信息

纯度

表达系统

标签

应用

生物活性

访问

不含动物源

不含载体蛋白

种属

存储溶液