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重组人Rac1蛋白(ab51014)

Reviews (1)Q&A (1)References (1)
SDS-PAGE - Recombinant Human Rac1 protein (ab51014)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE

    描述

    • 产品名称

      重组人Rac1蛋白
      参阅全部 Rac1 蛋白酶

    • 纯度

      > 95 % SDS-PAGE.

    • 表达系统

      Escherichia coli

    • 蛋白长度

      Full length protein

    • 无动物成分

      No

    • 性质

      Recombinant

      • 种属

        Human

      • 序列

        MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKP VNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPE VRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIG AVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL

      • 氨基酸

        1 to 192

    相关产品

    技术指标

    Our Abpromise guarantee covers the use of ab51014 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 形式

      Liquid
    • Concentration information loading...

    制备和贮存

    • 稳定性和存储

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

      pH: 7.50
      Constituents: 0.0154% DTT, 0.316% Tris HCl, 0.0584% EDTA, 10% Glycerol (glycerin, glycerine)

    常规信息

    • 别名

      • Cell migration inducing gene 5 protein
      • Cell migration-inducing gene 5 protein
      • MGC111543
      • MIG5
      • Migration inducing gene 5
      • Migration inducing protein 5
      • p21 Rac1
      • p21-Rac1
      • Rac 1
      • RAC1
      • RAC1_HUMAN
      • Ras like protein TC25
      • Ras related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
      • Ras-like protein TC25
      • Ras-related C3 botulinum toxin substrate 1
      • Rho family small GTP binding protein Rac1
      • TC 25
      • TC25
      see all

    • 功能

      Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
      Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.

    • 组织特异性

      Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.

    • 序列相似性

      Belongs to the small GTPase superfamily. Rho family.

    • 结构域

      The effector region mediates interaction with DEF6.

    • 翻译后修饰

      AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.

    • 细胞定位

      Cell membrane. Melanosome. Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt

    图片

    • SDS-PAGE - Recombinant Human Rac1 protein (ab51014)
      SDS-PAGE - Recombinant Human Rac1 protein (ab51014)

      15% SDS-PAGE analysis of 3 µg ab51014.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (1)

    发表研究结果有使用 ab51014？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab51014 被引用在 1 文献中.

    • Tzou FY  et al. Dihydroceramide desaturase regulates the compartmentalization of Rac1 for neuronal oxidative stress. Cell Rep 35:108972 (2021). PubMed: 33852856

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    RAC1 activity assay

     Excellent
    Abreviews
    abreview image
    Application
    Functional Studies
    This product is good for functional assays.

    I step up an assay using RAC1 and Bodipy-GDP, a fluorescent analog of GDP. The fluorescence of Bodipy-GDP is quenched in solution and increases upon binding to RAC1. The nucleotide exchange capacity of RAC1 was then tested by adding TIAM1, a guanine exchange factor with selectivity for RAC1. Fluorescence intensity (FI) was recorded for 25 min.
    Addition of buffer (vehicle) to the mixture of RAC1 and Bodipy-GDP resulted in the expected slow increase in FI due to RAC1 own's ability to exchange GDP. Whereas addition of TIAM resulted in a far quicker increase in FI.
    Both results indicate the functionality of RAC1 in this assay.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 03 2016

    Question

    Sehr geehrte Damen und Herren, wir möchten in unserer Arbeitsgruppe beginnen, mit der NADPH-oxidase in Makrophagen (Rattenmakrophagen NR8383, ev. auch humane Monozyten/Makrophagen U937) zu arbeiten und sind auf der Suche nach Antikörpern gegen ihre Bestandteile (p47PHOX, p40PHOX, p67PHOX, P22PHOX, gp91PHOX, Rac1/2, Rho-GDI, …), sowie (falls möglich) die Einzelkomponenten selber (für die Arbeit im zellfreien System). Können Sie uns weiterhelfen? Über ein Angebot würden wir uns freuen.

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    Answer

    Vielen Dank für Ihre Anfrage. Gegen die genannten NADPH-Oxidase-Komponenten haben wir folgende Antikörper bzw. Proteine in unserem Katalog: Click here (or use the following: https://www.abcam.com/NOXA2p67phox-antibody-EPR5064-ab109366.html). Click here (or use the following: https://www.abcam.com/NOX2gp91phox-antibody-ab80508.html). Click here (or use the following: https://www.abcam.com/Rac1-antibody-23A8-ab33186.html). Click here (or use the following: https://www.abcam.com/RAC2-antibody-ab2244.html). Click here (or use the following: https://www.abcam.com/RhoGDI-antibody-OTI1F2-ab118159.html). Click here (or use the following: https://www.abcam.com/Cytochrome-b245-Light-Chainp22-phox-antibody-ab75941.html). Click here (or use the following: https://www.abcam.com/p40-phox-antibody-ab115855.html). Click here (or use the following: https://www.abcam.com/p40-phox-phospho-T154-antibody-ab59355.html). Click here (or use the following: https://www.abcam.com/NCF1p47-phox-phospho-S328-antibody-ab111855.html). Click here (or use the following: https://www.abcam.com/NCF1p47-phox-antibody-ab795.html). Click here (or use the following: https://www.abcam.com/Human-NCF4-full-length-protein-ab88031.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-NCF1p47-phox-protein-ab98054.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-NOXA2p67phox-protein-ab117065.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-NOX2gp91phox-protein-ab114183.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-Rac1-protein-ab84657.html). Click here (or use the following: https://www.abcam.com/Human-Rac1-full-length-protein-ab40471.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-Rac1-protein-ab89246.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-Rac1-protein-ab82670.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-Rac1-protein-ab51014.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-RAC2-protein-ab84655.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-RAC2-protein-ab78684.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-RAC2-protein-ab87665.html). Vielleicht interessiert Sie in diesem Zusammenhang auch einer unserer Assaykits (NADP/NADPH): Click here (or use the following: https://www.abcam.com/NADPNADPH-Assay-Kit-ab65349.html). Wir geben alle getesteten Spezies und Anwendungen auf unseren Produktdatenblättern an und aktualisieren diese sobald wir neue Informationen erhalten. Falls sich herausstellt, dass der Antikörper nicht so funktioniert, wie auf dem Datenblatt beschrieben und Sie sich innerhalb von sechs Monaten mit Ihrem Problem an uns wenden, werden wir Ihnen gerne Vorschläge zur Optimierung Ihres Protokolls oder gegebenenfalls einen Ersatz oder eine Gutschrift schicken. Falls der Antikörper in einer bisher nicht getesteten Anwendung oder Spezies verwendet wird (z.B. für ab111855 oder ab115855), qualifizieren Sie sich für unser Testprogramm: Bei diesem Angebot bekommen Sie einen 100%igen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen: https://www.abcam.com/collaborationdiscount. Falls Sie daran interessiert sind oder weitere Fragen haben, kontaktieren Sie bitte unseren wissenschaftlichen Dienst. Die oben genannten Antikörper sind nur eine Auswahl und wir haben noch weitere gegen die entsprechenden Zielproteine erhältlich, die für unterschiedliche Anwendungen getestet und garantiert sind. Bei einer detaillierteren Suche bzw. Beratung bin ich Ihnen gerne weiter behilflich. Gerne senden wir Ihnen auch gerne ein Angebot zu, sobald Sie eine konkrete Auswahl getroffen haben. Ich hoffe, diese Informationen helfen Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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