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AB123186

重组人 Pyrophosphatase 1 蛋白 (His tag N-Terminus)

Recombinant Human Pyrophosphatase 1 protein (His tag N-Terminus)

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Recombinant Human Pyrophosphatase 1 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 289 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

查看别名

IOPPP, PP, PPA1, Inorganic pyrophosphatase, Pyrophosphate phospho-hydrolase, PPase

1 Images
SDS-PAGE - Recombinant Human Pyrophosphatase 1 protein (His tag N-Terminus) (AB123186)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Pyrophosphatase 1 protein (His tag N-Terminus) (AB123186)

15% SDS-PAGE showing ab123186 (3μg).

关键信息

纯度

>95% SDS-PAGE

ab123186 is purified using conventional chromatography techniques.

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

Mass Spec, SDS-PAGE

applications

生物活性

No

访问

Q15181

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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搭配使用产品

Reagents, Controls & Accessories

AB325994

Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color) (AB325994)

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Proteins & Peptides

AB128451

Recombinant Human DHODH protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human DHODH protein (His tag N-Terminus) (AB128451)

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Proteins & Peptides

AB132352

Recombinant Human Siah2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Siah2 protein (GST tag N-Terminus) (AB132352)

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Assay Kits

AB313929

Human Pyrophosphatase 1 ELISA Kit

Sandwich ELISA - Human Pyrophosphatase 1 ELISA Kit (AB313929)

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Primary Antibodies

AB250366

Anti-Pyrophosphatase 1 antibody [EPR11282] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Pyrophosphatase 1 antibody [EPR11282] - BSA and Azide free (AB250366)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMSGFSTEERAAPFSLEYRVFLKNEKGQYISPFHDIPIYADKDVFHMVVEVPRWSNAKMEIATKDPLNPIKQDVKKGKLRYVANLFPYKGYIWNYGAIPQTWEDPGHNDKHTGCCGDNDPIDVCEIGSKVCARGEIIGVKVLGILAMIDEGETDWKVIAINVDDPDAANYNDINDVKRLKPGYLEATVDWFRRYKVPDGKPENEFAFNAEFKDKDFAIDIIKSTHDHWKALVTKKTNGKGISCMNTTLSESPFKCDPDAARAIVDALPPPCESACTVPTDVDKWFHHQKN","proteinLength":"Full Length","predictedMolecularWeight":"35.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":289,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q15181","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

形式
Liquid
运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Pyrophosphatase 1 also known as iPPase or inorganic pyrophosphatase 1 is an enzyme responsible for the hydrolysis of inorganic pyrophosphate (PPi) into two phosphate ions. This reaction is energetically favorable and provides the driving force for many biosynthetic processes. Pyrophosphatase 1 is a soluble cytosolic enzyme with a molecular mass of about 33 kDa. It is expressed in various tissues especially high in metabolically active cells like those found in the liver and kidney.
Biological function summary

Pyrophosphate hydrolysis by pyrophosphatase 1 plays a critical role in energy metabolism and phosphate recycling within cells. This enzyme functions independently and is not part of a larger complex. Its activity maintains the balance of phosphate compounds in the cytosol. This balance is important for cellular functions like DNA and RNA synthesis where the removal of PPi is necessary to drive these reactions forward.

Pathways

Pyrophosphatase 1 influences several key metabolic routes such as nucleotide and protein biosynthesis. The enzyme ensures the continuation of these pathways by keeping PPi concentrations low which is important for reactions like aminoacyl-tRNA synthetase activity. Pyrophosphatase 1 connects with enzymes like polymerases and ligases through these pathways facilitating a range of anabolic processes by removing inhibitory PPi byproducts.

The dysregulation of pyrophosphatase 1 can impact conditions like hypophosphatasia and some forms of arthritis. In hypophosphatasia defective function of alkaline phosphatase leads to inadequate pyrophosphate breakdown affecting bone mineralization. Pyrophosphatase 1 by regulating PPi indirectly influences the symptoms when phosphate balance is disrupted. Furthermore its role in phosphate metabolism associates pyrophosphatase 1 with pathways involving matrix proteins that are implicated in mineralization disorders.
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常规信息

序列相似性

Belongs to the PPase family.

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产品实验方案

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靶点信息

See full target information PPA1
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