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AB180276

重组人Pur B蛋白

Recombinant Human Pur B protein

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Recombinant Human Pur B protein is a Human Full Length protein, in the 1 to 312 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

查看别名

Transcriptional regulator protein Pur-beta, Purine-rich element-binding protein B, PURB

1 Images
SDS-PAGE - Recombinant Human Pur B protein (AB180276)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Pur B protein (AB180276)

15% SDS-PAGE analysis of ab180276 (3μg).

关键信息

纯度

>95% SDS-PAGE

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

Mass Spec, SDS-PAGE

applications

生物活性

No

访问

Q96QR8

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMADGDSGSERGGGGGPCGFQPASRGGGEQETQELASKRLDIQNKRFYLDVKQNAKGRFLKIAEVGAGGSKSRLTLSMAVAAEFRDSLGDFIEHYAQLGPSSPEQLAAGAEEGGGPRRALKSEFLVRENRKYYLDLKENQRGRFLRIRQTVNRGGGGFGAGPGPGGLQSGQTIALPAQGLIEFRDALAKLIDDYGGEDDELAGGPGGGAGGPGGGLYGELPEGTSITVDSKRFFFDVGCNKYGVFLRVSEVKPSYRNAITVPFKAWGKFGGAFCRYADEMKEIQERQRDKLYERRGGGSGGGEESEGEEVDED","proteinLength":"Full Length","predictedMolecularWeight":"35.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":312,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96QR8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Pur B also known as purine-rich element-binding protein B is involved in DNA binding and transcriptional regulation. It is a 40 kDa protein localized in the nucleus and highly expressed in tissues with active cellular proliferation like the brain and the heart. Pur B associates with the purine-rich single-stranded DNA influencing the function and stability of genetic elements within these tissues.
Biological function summary

Pur B plays a significant role in cell growth and differentiation. It forms part of a multi-protein complex essential for regulating the expression of genes vital for cellular processes. The complex influences transcriptional activity altering how certain genes are turned on or off which ultimately impacts cellular function and development.

Pathways

Pur B is integral to the regulation of transcription and nucleic acid processes. It is involved in pathways like the response to stress and regulation of apoptosis. Within these pathways Pur B interacts with other transcription factors such as Pur A contributing to the precise control of gene expression essential for maintaining cellular integrity and responding to physiological changes.

Pur B displays an association with neurodegenerative diseases and cancer. Dysregulation of Pur B levels or function links to Alzheimer’s disease where it affects neural development and function often alongside Pur A. Additionally abnormal Pur B activity is observed in certain cancers potentially due to its role in gene transcription involved in cell proliferation where it might interact with oncogenic factors exacerbating tumor progression.
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特殊说明

形式

Liquid

附加说明

ab180276 is purified using conventional chromatography techniques.

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常规信息

功能

Transcriptional regulator which can act as an activator or a repressor. Represses the transcription of ACTA2 in fibroblasts and smooth muscle cells via its ability to interact with the purine-rich strand of a MCAT- containing element in the 5' flanking region of the gene. Represses the transcription of MYOCD, capable of repressing all isoforms of MYOCD but the magnitude of the repressive effects is most notable for the SMC- specific isoforms. Promotes hepatic glucose production by activating the transcription of ADCY6, leading to cAMP accumulation, increased PKA activity, CREB activation, and increased transcription of PCK1 and G6PC genes (By similarity). Has capacity to bind repeated elements in single-stranded DNA such as the purine-rich single strand of the PUR element located upstream of the MYC gene (PubMed : 1448097). Participates in transcriptional and translational regulation of alpha-MHC expression in cardiac myocytes by binding to the purine-rich negative regulatory (PNR) element Modulates constitutive liver galectin-3 gene transcription by binding to its promoter. May play a role in the dendritic transport of a subset of mRNAs (By similarity).

序列相似性

Belongs to the PUR DNA-binding protein family.

亚细胞定位

Nucleus

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Transcriptional regulator which can act as an activator or a repressor. Represses the transcription of ACTA2 in fibroblasts and smooth muscle cells via its ability to interact with the purine-rich strand of a MCAT- containing element in the 5' flanking region of the gene. Represses the transcription of MYOCD, capable of repressing all isoforms of MYOCD but the magnitude of the repressive effects is most notable for the SMC- specific isoforms. Promotes hepatic glucose production by activating the transcription of ADCY6, leading to cAMP accumulation, increased PKA activity, CREB activation, and increased transcription of PCK1 and G6PC genes (By similarity). Has capacity to bind repeated elements in single-stranded DNA such as the purine-rich single strand of the PUR element located upstream of the MYC gene (PubMed : 1448097). Participates in transcriptional and translational regulation of alpha-MHC expression in cardiac myocytes by binding to the purine-rich negative regulatory (PNR) element Modulates constitutive liver galectin-3 gene transcription by binding to its promoter. May play a role in the dendritic transport of a subset of mRNAs (By similarity).
See full target information PURB
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