Key features and details
- Expression system: Escherichia coli
- Purity: > 80% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Inhibition Assay
参阅全部 PTPN13/FAP-1 蛋白酶
Specific Activity: 5 U/µg. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3mM DTT, 100 mM NaCl, 50 mM pNPP. The specific activity of PTPN13/FAP-1 was determined using pNPP. Enzyme reaction condition: 20 mM pNPP, 5 min incubation at 30°C, 1µg enzyme.
纯度> 80 % SDS-PAGE.
Purified using glutathione-agarose beads.
预测分子量72 kDa including tags
氨基酸2091 to 2490
标签GST tag N-Terminus
Our Abpromise guarantee covers the use of ab42581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product was previously labelled as PTPN13
Concentration information loading...
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.307% Glutathione, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 50% Glycerol (glycerin, glycerine), 0.435% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- APO1/CD95 (Fas) associated phosphatase
- FAP 1
相关性PTPN13 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP is a large protein that possesses a PTP domain at C-terminus, and multiple noncatalytic domains, which include a domain with similarity to band 4.1 superfamily of cytoskeletal associated proteins, a region consisting of five PDZ domains, and a leucine zipper motif. This PTP was found to interact with, and dephosphorylate Fas receptor, as well as I-kappa-B-alpha through the PDZ domains, which suggested its role in Fas mediated programmed cell death. This PTP was also shown to interact with GTPase-activating protein, and thus may function as a regulator of Rho signaling pathway.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.