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AB51277

重组人 PTP1B 蛋白 (Active) (Tag Free)

Recombinant human PTP1B protein (Active) (Tag Free)

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(11 Publications)

Recombinant human PTP1B protein (Active) (Tag Free) is a Human Fragment protein, in the 1 to 321 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.

查看别名

PTP1B, PTPN1, Tyrosine-protein phosphatase non-receptor type 1, Protein-tyrosine phosphatase 1B, PTP-1B

1 Images
SDS-PAGE - Recombinant human PTP1B protein (Active) (Tag Free) (AB51277)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PTP1B protein (Active) (Tag Free) (AB51277)

3ug of reduced ab51277 on SDS-PAGE, stained with Coomassie Blue.

关键信息

纯度

>95% SDS-PAGE

This protein was purified by conventional column chromatographic techniques.

表达系统

Escherichia coli

标签

Tag free

应用

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学活性

Specific activity is > 10,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Activity Assay:

  1. Prepare a 180ul assay buffer into a suitable container: The concentrations are 11mM Tris-HCl pH7.5, 55mM NaCl, 2.2mM DTT, 1.1mM MnCl2, 1.1mM EDTA, 33mM pNPP.
  2. Equilibrate to 37℃ and monitor the A405nm until value is constant using a spectrophotometer.
  3. Add 20ul of recombinant PTP-1B protein 12.5ug/ml in 0.1% BSA/DW.
  4. Record the absorbance A405nm for 5 minutes.
访问

P18031

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.5 Constituents: 20% Glycerol (glycerin, glycerine), 0.395% Tris HCl, 0.0292% EDTA, 0.0156% 2-Mercaptoethanol, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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搭配使用产品

Reagents, Controls & Accessories

AB325997

Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color) (AB325997)

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Proteins & Peptides

AB60884

Recombinant Src protein (GST tag N-Terminus)

Functional Studies - Recombinant Src protein (AB60884)

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Proteins & Peptides

AB177261

Recombinant human LRRK2 protein (GST tag N-Terminus)

Functional Studies - Recombinant human LRRK2 protein (AB177261)

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Assay Kits

AB65622

Phosphate Assay Kit (Colorimetric)

Functional Studies - Phosphate Assay Kit (Colorimetric) (AB65622)

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Primary Antibodies

AB244207

Anti-PTP1B antibody [EPR22474]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [EPR22474] (AB244207)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Activity assay information can be found in the activity section of this datasheet.</p>" } } }
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产品详情

Concentration determined by Bradford assay.
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序列信息

[{"linker":null,"sequence":"MEMEKEFEQIDKSGSWAAIYQDIRHEASDFPCRVAKLPKNKNRNRYRDVSPFDHSRIKLHQEDNDYINASLIKMEEAQRSYILTQGPLPNTCGHFWEMVWEQKSRGVVMLNRVMEKGSLKCAQYWPQKEEKEMIFEDTNLKLTLISEDIKSYYTVRQLELENLTTQETREILHFHYTTWPDFGVPESPASFLNFLFKVRESGSLSPEHGPVVVHCSAGIGRSGTFCLADTCLLLMDKRKDPSSVDIKKVLLEMRKFRMGLIQTADQLRFSYLAVIEGAKFIMGDSSVQDQWKELSHEDLEPPPEHIPPPPRPPKRILEPHN","proteinLength":"Fragment","predictedMolecularWeight":"37.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":321,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P18031","tags":[]}]
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性能和储存信息

形式
Liquid
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Protein tyrosine phosphatase 1B (PTP1B) is an enzyme involved in the dephosphorylation of tyrosine residues in proteins. Known also as PTPN1 this protein has a molecular weight of approximately 50 kDa. PTP1B is widely expressed in tissues but is highly present in the liver muscle and adipose tissue. It plays a critical role in regulating signal transduction pathways by removing phosphate groups from phosphotyrosine residues on target proteins. This action generally serves as a 'turn-off' switch for various signaling pathways.
Biological function summary

PTP1B plays important roles in glucose and lipid metabolism. It is not generally considered a part of a complex functioning instead as a singular enzyme. Its main action involves the negative regulation of the insulin signaling pathway by dephosphorylating the insulin receptor as well as its associated substrates. This regulation helps maintain normal levels of insulin sensitivity influencing cellular glucose uptake and metabolism.

Pathways

PTP1B has important functions in the insulin and leptin signaling pathways. It has a regulatory influence in these pathways affecting key metabolic processes. In the insulin signaling pathway PTP1B closely interacts with the insulin receptor as well as with insulin receptor substrates (IRS). Its dephosphorylating activity can lead to decreased insulin sensitivity. In the leptin pathway PTP1B modulates leptin signaling by dephosphorylating the Janus kinase 2 (JAK2) another protein involved in the control of energy balance and metabolism.

Excessive activity of PTP1B links with disorders such as type 2 diabetes and obesity. Overactivity of PTP1B can lead to insulin resistance contributing to the pathogenesis of type 2 diabetes. In obesity PTP1B may interfere with the normal activity of leptin a hormone critical for regulating energy balance. Its interaction with the insulin receptor and JAK2 makes PTP1B a target for therapeutic interventions aimed at improving insulin sensitivity and energy homeostasis.
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常规信息

功能

Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.

序列相似性

Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.

翻译后修饰

Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.. Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50.. S-nitrosylation of Cys-215 inactivates the enzyme activity.. Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity.

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产品实验方案

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靶点信息

Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.
See full target information PTPN1
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文献 (11)

Recent publications for all applications. Explore the full list and refine your search

Journal of enzyme inhibition and medicinal chemistry 39:2360063 PubMed38873930

2024

New natural protein tyrosine phosphatase 1B inhibitors from .

Applications

Unspecified application

Species

Unspecified reactive species

Xianting Wang,Yidan Deng,Jianmei Wang,Lin Qin,Yimei Du,Qianru Zhang,Di Wu,Xingdong Wu,Jian Xie,Yuqi He,Daopeng Tan

Journal of enzyme inhibition and medicinal chemistry 38:2281263 PubMed37965892

2023

New dammarane-type triterpenoids from hydrolyzate of total saponins with protein tyrosine phosphatase 1B inhibitory activity.

Applications

Unspecified application

Species

Unspecified reactive species

Daopeng Tan,Jianmei Wang,Xianting Wang,Lin Qin,Yimei Du,Changkuo Zhao,Peijun Liu,Qianru Zhang,Feifei Ma,Jian Xie,Di Wu,Yuqi He

Journal of ginseng research 47:274-282 PubMed36926615

2023

Ginsenoside compound K reduces ischemia/reperfusion-induced neuronal apoptosis by inhibiting PTP1B-mediated IRS1 tyrosine dephosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Fu,Liang Yu,Qian Yu,Nengwei Yu,Fei Xu,Suping Li

International journal of molecular sciences 24: PubMed36901928

2023

Dual PTP1B/TC-PTP Inhibitors: Biological Evaluation of 3-(Hydroxymethyl)cinnoline-4()-Ones.

Applications

Unspecified application

Species

Unspecified reactive species

Kira V Derkach,Maxim A Gureev,Anastasia A Babushkina,Vladimir N Mikhaylov,Irina O Zakharova,Andrey A Bakhtyukov,Viktor N Sorokoumov,Alexander S Novikov,Mikhail Krasavin,Alexander O Shpakov,Irina A Balova

International journal of molecular sciences 24: PubMed36769221

2023

HS- and Redox-State-Mediated PTP1B S-Sulfhydration in Insulin Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Chin Lin,Wan-Ting Zeng,Der-Yen Lee

Biomeditsinskaia khimiia 68:427-436 PubMed36573409

2022

[Characterization and biological activity of new 4-oxo-1,4-dihydrocinnoline-based inhibitors of the tyrosine phosphatase PTP1B and TCPTP].

Applications

Unspecified application

Species

Unspecified reactive species

K V Derkach,I O Zakharova,A A Bakhtyukov,V N Sorokoumov,V S Kuznetsova,A O Shpakov

Oxidative medicine and cellular longevity 2021:7807046 PubMed34707780

2021

Antioxidant, Anti-Inflammatory, and Antidiabetic Activities of Bioactive Compounds from the Fruits of Based on Network Pharmacology Prediction.

Applications

Unspecified application

Species

Unspecified reactive species

Yuwei Wang,Jianxiu Zhai,Dan Yang,Na Han,Zhe Liu,Zhihui Liu,Sikai Li,Jun Yin

Communications biology 4:248 PubMed33627831

2021

βA1-crystallin regulates glucose metabolism and mitochondrial function in mouse retinal astrocytes by modulating PTP1B activity.

Applications

Unspecified application

Species

Unspecified reactive species

Sayan Ghosh,Haitao Liu,Meysam Yazdankhah,Nadezda Stepicheva,Peng Shang,Tanuja Vaidya,Stacey Hose,Urvi Gupta,Michael Joseph Calderon,Ming-Wen Hu,Archana Padmanabhan Nair,Joseph Weiss,Christopher S Fitting,Imran A Bhutto,Santosh Gopi Krishna Gadde,Naveen Kumar Naik,Chaitra Jaydev,Gerard A Lutty,James T Handa,Ashwath Jayagopal,Jiang Qian,José-Alain Sahel,Dhivyaa Rajasundaram,Yuri Sergeev,J Samuel Zigler,Swaminathan Sethu,Simon Watkins,Arkasubhra Ghosh,Debasish Sinha

Molecules (Basel, Switzerland) 25: PubMed32867374

2020

Benzyl Furanones and Pyrones from the Marine-Derived Fungus Induced by Chemical Epigenetic Modification.

Applications

Unspecified application

Species

Unspecified reactive species

Jing-Shuai Wu,Xiao-Hui Shi,Ya-Hui Zhang,Chang-Lun Shao,Xiu-Mei Fu,Xin Li,Guang-Shan Yao,Chang-Yun Wang

Marine drugs 18: PubMed32110865

2020

New Thiodiketopiperazine and 3,4-Dihydroisocoumarin Derivatives from the Marine-Derived Fungus .

Applications

Unspecified application

Species

Unspecified reactive species

Jing-Shuai Wu,Xiao-Hui Shi,Guang-Shan Yao,Chang-Lun Shao,Xiu-Mei Fu,Xiu-Li Zhang,Hua-Shi Guan,Chang-Yun Wang
View all publications
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