重组人PRL-1蛋白(ab42567)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag C-Terminus
- Suitable for: Inhibition Assay
描述
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产品名称
重组人PRL-1蛋白
参阅全部 PRL-1 蛋白酶 -
生物活性
0.45 U/ug. One unit will hydrolyze 1 pmol 6, 8-difluoro-4-methyl umbelliferyl phosphate (DiFMUP) per minute at pH 6.3 and 30?C. Assay buffer: 50 mM Bis-Tris, pH 6.3, 2 mM EDTA, 2 mM DTT, 100 mM DiFMUP. -
纯度
> 95 % SDS-PAGE. -
表达系统
Escherichia coli -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
20 kDa including tags -
氨基酸
2 to 173 -
标签
His tag C-Terminus
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技术指标
Our Abpromise guarantee covers the use of ab42567 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Inhibition Assay
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形式
Liquid -
补充说明
0.45 U/ug. One unit will hydrolyze 1 pmol 6, 8-difluoro-4-methyl umbelliferyl phosphate (DiFMUP) per minute at pH 6.3 and 30?C. Assay buffer: 50 mM Bis-Tris, pH 6.3, 2 mM EDTA, 2 mM DTT, 100 mM DiFMUP. -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.307% Glutathione, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 0.0584% EDTA, 50% Glycerol (glycerin, glycerine), 0.435% Sodium chloride
常规信息
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别名
- HH 72
- HH72
- Hypothetical protein DKFZp779M0721
see all -
功能
Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. May play a role in the development and maintenance of differentiating epithelial tissues. Enhances cell proliferation, cell motility and invasive activity, and promotes cancer metastasis. -
组织特异性
Expressed in bone marrow, lymph nodes, T lymphocytes, spleen, thymus and tonsil. Overexpressed in tumor cell lines. -
序列相似性
Belongs to the protein-tyrosine phosphatase family.
Contains 1 tyrosine-protein phosphatase domain. -
发展阶段
Expressed in fetal liver. -
翻译后修饰
Farnesylated. Farnesylation is required for membrane targeting. Unfarnesylated forms are shifted into the nucleus. -
细胞定位
Cell membrane. Early endosome. Endoplasmic reticulum. Cytoplasm. Cytoplasm > cytoskeleton > spindle. And mitotic spindle. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab42567 尚未被引用在任何文献中。